Comparison of rapid card agglutination test with the complement fixation test for diagnosis of Anaplasma marginale infection in Cattle in Colombia

1977 ◽  
Vol 2 (2) ◽  
pp. 167-177 ◽  
Author(s):  
R.A. Todorovic ◽  
R.F. Long ◽  
B.R. McCallon
1994 ◽  
Vol 6 (2) ◽  
pp. 200-206 ◽  
Author(s):  
S. J. Rodgers ◽  
R. D. Welsh ◽  
M. E. Stebbins

The prevalence of anaplasmosis in Oklahoma cattle was determined on the basis of the standardized Anaplasma marginale complement fixation test on 20,155 sera submitted to the Oklahoma Animal Disease Diagnostic Laboratory during a 15-year period. Rates of seropositivity ranged from 4.7% to 17.6% on samples submitted for anaplasmosis testing of adult cows. The geographic distribution of recorded cases of anaplasmosis was 35 Oklahoma counties in 1977 and 48 Oklahoma counties in 1991.


1977 ◽  
Vol 79 (2) ◽  
pp. 181-192 ◽  
Author(s):  
J. Brodie

SUMMARYIn a previous paper (Brodie, 1977) the value of the Widal test in the diagnosis of typhoid fever was shown to be limited. Evaluation of possible alternative tests showed that:(1) the sensitivity of the anti-human globulin (Coombs') test appeared greater than that of the agglutination test but the length of time (48 h) before results were available rendered it of little value in rapid diagnosis;(2) the complement fixation test offered no particular help towards diagnosis;(3) immunized and non-immunized typhoid fever patients developed fimbrial antibodies, as also did immunized healthy individuals. In this latter group, however, those immunized with alcoholized TAB vaccine had higher antibody titres to fimbrial antigen than those immunized with heat-killed phenolized vaccine.


1947 ◽  
Vol 45 (1) ◽  
pp. 50-55 ◽  
Author(s):  
R. Th. Scholtens

The titres of the complement-fixation test and the agglutination test run parallel in rabbit sera, but not in human sera. These facts are explained in connexion with the specificity of complement.


1976 ◽  
Vol 77 (3) ◽  
pp. 369-376 ◽  
Author(s):  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught ◽  
M. J. Dalling ◽  
G. S. Allan

SUMMARYA radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to 1gM, a characteristic which may make it more suitable than the complement fixation test (OFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.


Author(s):  
A. Yahia ◽  
K. Hamrat ◽  
K. Saidani ◽  
R. Kaidi

In order to determine the prevalence of bovine brucellosis and the factors affecting its persistence in the province of Djelfa (Algeria), a sero-epidemiological study was carried out on a total of 10827 cattle (7346 females and 3481 males) during the decade from 2004 to 2013. The sera were screened by card agglutination test, and the positive samples were confirmed by the complement fixation test. Some factors (year, age, breed and sex) were considered. Results found an average prevalence of brucellosis during these ten years of 1.4% with a highly significant difference according to year and sex (P Lass Than 0.05). A prevalence of 1.58% for females against a prevalence of 1.03% for males was observed. No significant difference was observed for the different age groups (P>0.05) in the seroprevalence of brucellosis. A higher prevalence of infection (1.53%) was found in imported breeds compared to local and cross breeds with (1.39%) and (1.35%) respectively. The effect of the breed is highly significant (P Lass Than 0.05). In conclusion the bovine brucellosis persists in the province of Djelfa and it is necessary to implement a scheme to fight and prevent this infection.


1955 ◽  
Vol 53 (1) ◽  
pp. 63-75 ◽  
Author(s):  
H. Barber

Summary1. Direct conglutination has been demonstrated withRickettsia burneti.2. Sera from human volunteers inoculated with killed suspensions ofR. burnetiand from persons who had recovered from an attack of Q fever as well as sera from a small number of healthy blood donors were all examined by the direct conglutination reaction, the haemolytic-complement fixation test and the direct agglutination test. A good correlation was found between the titres of the direct conglutination reaction and the haemolytic complement-fixation test but the results with the direct agglutination test were unreliable.


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