scholarly journals Antibodies and the Aberdeen typhoid outbreak of 1964: II. Coombs', complement fixation and fimbrial agglutination tests

1977 ◽  
Vol 79 (2) ◽  
pp. 181-192 ◽  
Author(s):  
J. Brodie
Keyword(s):  
Typhoid Fever ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Rapid Diagnosis ◽  
Agglutination Test ◽  
Fixation Test ◽  
Coombs Test ◽  
Healthy Individuals ◽  
Widal Test ◽  
Antibody Titres

SUMMARYIn a previous paper (Brodie, 1977) the value of the Widal test in the diagnosis of typhoid fever was shown to be limited. Evaluation of possible alternative tests showed that:(1) the sensitivity of the anti-human globulin (Coombs') test appeared greater than that of the agglutination test but the length of time (48 h) before results were available rendered it of little value in rapid diagnosis;(2) the complement fixation test offered no particular help towards diagnosis;(3) immunized and non-immunized typhoid fever patients developed fimbrial antibodies, as also did immunized healthy individuals. In this latter group, however, those immunized with alcoholized TAB vaccine had higher antibody titres to fimbrial antigen than those immunized with heat-killed phenolized vaccine.

scholarly journals Antibodies and the Aberdeen typhoid outbreak of 1964: I. The Widal reaction

1977 ◽  
Vol 79 (2) ◽  
pp. 161-180 ◽  
Author(s):  
B J. Brodie
Keyword(s):  
Typhoid Fever ◽  
Antibody Titre ◽  
Research Programme ◽  
Healthy Individuals ◽  
Widal Test ◽  
Antibody Titres ◽  
Discharge From Hospital ◽  
Somatic Antigens ◽  
High Antibody Titre ◽  
Do So

SUMMARYThe outbreak of typhoid fever in Aberdeen during 1964 (Walker, 1965) presented an opportunity to study the antibody titres of typhoid fever patients and of TAB immunized individuals to obtain further knowledge concerning the behaviour of these titres with the passage of time.This paper gives an abbreviated version of part of a research programme which followed the Aberdeen typhoid outbreak of 1964.The antibody titres of patients were followed up for a period of 2 years after discharge from hospital and the findings have been compared with those in TAB immunized healthy individuals. The following points emerged:(1) The value of the Widal test as an aid to diagnosis was limited;(2) the flagellar antibody titre in patients' sera provided a more reliable aid towards diagnosis than did the somatic antibody titre;(3) the response of immunized and non-immunized patients to the somatic antigens was poor and often delayed well into the period following discharge from hospital;(4) titres of 1/40 and over for Vi agglutinins were present in immunized and non-immunized patients for at least 12 months after discharge without their beingS. typhiexcretors;(5) Vi agglutinin titres as high as 1/40 were present in TAB immunized healthy individuals and also in members of the general public;(6) the presence ofS. typhisepticaemia need not result in a high antibody titre; (7) patients who relapse, may do so without enhancement of previous antibody titres and may relapse even in the presence of earlier appreciable titres.

scholarly journals Agglutination and complement fixation in human and rabbit typhoid and dysentery sera and the specificity of complement

1947 ◽  
Vol 45 (1) ◽  
pp. 50-55 ◽  
Author(s):  
R. Th. Scholtens
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Fixation Test ◽  
Human Sera

The titres of the complement-fixation test and the agglutination test run parallel in rabbit sera, but not in human sera. These facts are explained in connexion with the specificity of complement.

scholarly journals The relevance of the anti-human globulin (Coombs) test and the complement-fixation test in the diagnosis of brucellosis

1970 ◽  
Vol 68 (2) ◽  
pp. 173-187 ◽  
Author(s):  
D. G. McDevitt
Keyword(s):  
Private Practice ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Coombs Test ◽  
Diagnostic Significance ◽  
Practice Experience ◽  
Veterinary Surgeons ◽  
The Relationship ◽  
Diagnostic Importance

SummaryThe relationship between the serological findings for brucellosis and the epidemiological factors has been studied in veterinary surgeons in Northern Ireland. The anti-human globulin (Coombs) test and the complement-fixation test for brucella were used in two groups of veterinary surgeons, those self-employed and those employed by the Ministry of Agriculture.Significant serological differences were found to exist between the two groups. Those in private practice showed changes related to age, cattle skin rash, reactions to S. 19 vaccine accidents and symptoms suggestive of brucellosis in the past or the last year. But those working for the Ministry only showed titre changes related to the length of their private practice experience before joining the Ministry. In neither group was there a relationship between serological findings and the type of milk drunk or any particular group of symptoms suggestive of brucellosis.The findings indicate that high titres to brucella by the Coombs and complement-fixation test can occur in people repeatedly exposed to infection at work. Titres which would be of diagnostic importance in the rest of the population may be of little diagnostic significance even when they are as high as 160 Coombs and 128 complement fixation.

scholarly journals Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis

1976 ◽  
Vol 77 (3) ◽  
pp. 369-376 ◽  
Author(s):  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught ◽  
M. J. Dalling ◽  
G. S. Allan
Keyword(s):  
Specific Antibody ◽  
Brucella Abortus ◽  
Bovine Serum ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Serological Test ◽  
Agglutination Test ◽  
Fixation Test ◽  
Bovine Brucellosis ◽  
Serum Agglutination

SUMMARYA radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to 1gM, a characteristic which may make it more suitable than the complement fixation test (OFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.

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