Characterisation of the outer membrane protein antigens of British field isolates of Moraxella bovis

1987 ◽  
Vol 15 (3) ◽  
pp. 181-189
Author(s):  
Jane M. Horsnell ◽  
Catherine M. Teale
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Protein Antigens ◽  
Field Isolates ◽  
Moraxella Bovis

scholarly journals Type-specific antigens of group A Neisseria meningitidis: lipopolysaccharide and heat-modifiable outer membrane proteins

1980 ◽  
Vol 28 (2) ◽  
pp. 451-458 ◽  
Author(s):  
W D Zollinger ◽  
R E Mandrell
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Neisseria Meningitidis ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Solid Phase ◽  
Outer Membrane Proteins ◽  
Surface Antigens ◽  
Protein Antigens ◽  
Group A

The solid-phase radioimmunoassay inhibition method was used to analyze the noncapsular surface antigens of group A Neisseria meningitidis for type specificity. By use of antisera prepared against group A strains, three serologically distinct lipopolysaccharide antigens and five outer membrane protein antigens were identified among group A strains from a variety of geographical origins. Two of the lipopolysaccharide antigens were unique to group A strains while the third was similar to those on strains of other meningococcal serogroups. Fractionation of outer membrane proteins in the presence of 2% sodium deoxycholate followed by quantitative inhibition of the typing reactions with the subfractions revealed that the protein responsible for type specificity was not the principal outer membrane protein, but, most likely, the 31,000-dalton, heat-modifiable outer membrane protein. Thus, although group A strains may share a common principal outer membrane protein, typing is feasible using other surface antigens. In a survey of 82 group A strains, 93% were typable with respect to outer membrane proteins.

scholarly journals Outer membrane protein antigens in an enzyme-linked immunosorbent assay for Salmonella enteric fever and meningococcal meningitis

1978 ◽  
Vol 7 (4) ◽  
pp. 372-378
Author(s):  
J E Sippel ◽  
H K Mamay ◽  
E Weiss ◽  
S W Joseph ◽  
W J Beasley
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Immunosorbent Assay ◽  
Outer Membrane Protein ◽  
Cell Envelope ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Antigens ◽  
Electrophoretic Mobilities ◽  
Highly Sensitive ◽  
Common Antigens

Outer membrane protein preparations were obtained from strains of Salmonella and Neisseria meningitidis. Solubilized cell envelope (CE) fractions from S. typhi and Salmonella groups A, B, C, and E had very similar electrophoretic mobilities on polyacrylamide gel, and common antigens were demonstrated by immunodiffusion. CE appeared to be a more satisfactory antigen than the more purified preparation (T/TEI) in the enzyme-linked immunosorbent assay (ELISA) with sera from typhoid and paratyphoid patients. With either antigen, however, the presence of antibodies was demonstrated in acute- and vonvalescent-phase sera. In the case of N. meningitidis infections, the crude (STA) and the more purified antigens (T/TEI) were equally satisfactory, and a rise in antibody titer could easily be demonstrated with paired acute- and convalescent-phase sera. The ELISA appears to be a simple but highly sensitive test for the detection of antibodies by using outer membrane protein antigens.

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