Bovine leukaemia virus: Rapid detection of proviral DNA by nested PCR in blood and organs of experimentally infected calves

1994 ◽  
Vol 42 (2-3) ◽  
pp. 191-204 ◽  
Author(s):  
K. Klintevall ◽  
A. Ballagi-Pordány ◽  
K. Näslund ◽  
S. Belák
Keyword(s):  
Nested Pcr ◽  
Rapid Detection ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus ◽  
Proviral Dna

scholarly journals Application of in situ PCR for the Detection of Bovine Leukaemia Virus (BLV) Infection in Dendritic Cell cultures

2014 ◽  
Vol 58 (3) ◽  
pp. 347-352 ◽  
Author(s):  
Ewelina Iwan ◽  
Maria Szczotka ◽  
Jacek Kuźmak
Keyword(s):  
Cell Cultures ◽  
Nested Pcr ◽  
Leukemia Virus ◽  
Positive Control ◽  
Adherent Cell ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus ◽  
Pcr Method

Abstract The aim of the study was to develop an in situ PCR (IS-PCR) method for detection of bovine leukemia virus (BLV) in cell cultures. Samples from five BLV positive and five BLV negative cows were collected and dendritic cells (DCs) from blood, bone marrow, spleen, and lymph node were cultured. Cultures prepared from healthy animals were infected with BLV. After two weeks, the cells were tested by nested PCR and IS-PCR for the presence of proviral DNA. As a positive control adherent cell line permanently infected with BLV was used. BLV was successfully detected by IS-PCR in DCs from naturally infected cattle and DCs infected in vitro. In control, non-infected DCs, the results of the reaction were negative. The results of provirus detection by IS-PCR were similar with these performed with nested PCR. Additionally, IS-PCR provides many advantages, like specific localisation of infection and smaller number of cells needed as template for PCR.

Detection of Bovine Leukaemia Virus Antibodies and Proviral DNA in Colostrum Replacers

2013 ◽  
Vol 62 (5) ◽  
pp. e60-e61 ◽  
Author(s):  
B. Choudhury ◽  
C. Finnegan ◽  
A. Phillips ◽  
M. Horigan ◽  
T. Pollard ◽  
...  
Keyword(s):  
Bovine Leukaemia Virus ◽  
Leukaemia Virus ◽  
Proviral Dna

A validated semi-nested PCR for rapid detection of scale drop disease virus (SDDV) in Asian sea bass (Lates calcarifer)

2019 ◽  
Vol 268 ◽  
pp. 37-41 ◽  
Author(s):  
Onanong Charoenwai ◽  
Watcharachai Meemetta ◽  
Molrudee Sonthi ◽  
Ha Thanh Dong ◽  
Saengchan Senapin
Keyword(s):  
Disease Virus ◽  
Nested Pcr ◽  
Rapid Detection ◽  
Sea Bass ◽  
Lates Calcarifer ◽  
Asian Sea Bass

Infectivity of bovine leukaemia virus infected cattle: An ELISA for detecting antigens expressed in in vitro cultured lymphocytes

1992 ◽  
Vol 30 (2-3) ◽  
pp. 137-150 ◽  
Author(s):  
J.A. Cowley ◽  
J.B. Molloy ◽  
C.K. Dimmock ◽  
P.J. Walker ◽  
A.G. Bruyeres ◽  
...  
Keyword(s):  
Infected Cattle ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus
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