Apparatus and method for increasing the sensitivity of a nuclear magnetic resonance probe

1987 ◽  
Vol 42 (6) ◽  
pp. ii
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nuclear Magnetic Resonance Probe ◽  
Nuclear Magnetic

scholarly journals The metabolism of the phosphonium analogue of choline in cultured cells. A useful nuclear-magnetic-resonance probe for membrane phosphatidylcholine

1976 ◽  
Vol 154 (1) ◽  
pp. 105-111 ◽  
Author(s):  
E Sim ◽  
C A Pasternak
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Cultured Cells ◽  
Head Group ◽  
Polar Head Group ◽  
A Minor ◽  
Nuclear Magnetic Resonance Probe ◽  
Synthesis And Degradation ◽  
Minor Extent ◽  
Nuclear Magnetic

1. Replacement of choline by the phosphonium analogue does not affect the growth rate of P815Y, NIL, 3T3, and SV40/3T3 cells in culture. 2. The fatty acid composition of the resulting phosphonium phosphatidylcholine is similar to that of phosphatidylcholine. 3. The rate of synthesis and degradation of phosphatidylcholine and of the phosphonium analogue are similar. 4. Phospholipid-exchange protein does not distinguish between phosphatidylcholine and the phosphonium analogue. 5. By contrast, incorporation of phosphonium choline into sphingomyelin occurs to only a minor extent. 6. It is concluded that, since the enzymes involved in the turnover of phosphatidylcholine do not discriminate between quaternary N and quaternary P in the polar head-group region, phosphonium choline should prove to be a useful probe for 31P nuclear-magnetic-resonance (n.m.r.) studies of natural membranes.

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