Comparison of short- and long-feed transmission of the cauliflower mosaic virus Cabb-S strain and SΔII hybrid by two species of aphid: Myzus persicae (Sulzer) and Brevicoryne brassicae (L.)

1990 ◽  
Vol 141 (6) ◽  
pp. 677-683 ◽  
Author(s):  
Y Bouchery ◽  
L Givord ◽  
P Monestiez
Keyword(s):  
Mosaic Virus ◽  
Myzus Persicae ◽  
Brevicoryne Brassicae ◽  
Cauliflower Mosaic Virus

The effect of DDT on Myzus persicae (SULZ.) and Brevicoryne brassicae (L.) (Aphididae) in relation to the spread of cauliflower mosaic and cabbage black ring spot viruses

1963 ◽  
Vol 53 (4) ◽  
pp. 779-784 ◽  
Author(s):  
G. D. Heathcote ◽  
J. Ward
Keyword(s):  
Myzus Persicae ◽  
Brevicoryne Brassicae ◽  
Black Ring ◽  
Cauliflower Mosaic Virus ◽  
Virus Diseases ◽  
Spot Virus ◽  
Treated Plant ◽  
Previously Treated ◽  
Ring Spot ◽  
Do So

Previous work in Britain has shown that insecticides, even systemic ones, fail to protect brassica plants in the seed-bed from virus diseases transmitted by aphids, and an explanation was sought through experiments carried out at Rothamsted. Observations on the effect on apterae and alates of Myzus persicae (Sulz.) of contact with leaves detached from cauliflower plants previously treated with a 0·2 per cent. DDT emulsion spray showed that exposure for up to 30 min. was insufficient to kill the majority of those tested, although many were temporarily incapacitated; alatae of Brevicoryne brassicae (L.) were even less affected.In cage experiments using infected and healthy cauliflower seedlings, apterae of M. persicae were able to transmit cauliflower mosaic virus (ClMV) and cabbage black ring spot virus (CBRSV) from infected to healthy seedlings when no spray was used, but failed to do so when the infected plants were sprayed with DDT; when alates were used in similar tests using only CBRSV, only low rates of transmission were obtained. In similar experiments using turnip seedlings and ClMV, transmission by apterae of M. persicae was high on untreated plants when the infector plant was itself untreated, but distinctly lower when it was sprayed with DDT; alates of M. persicae were found to transmit ClMV from a DDT-treated infector to untreated plants but not to a treated plant. Virus spread was thus reduced but not prevented by the use of DDT sprays. It is considered that these results lend support to the hypothesis that increase of virus within a seed-bed is preponderantly the result of the arrival, in succession, of many individual viruliferous alates from outside.

Epidemiology of Cauliflower Mosaic virus in South Australia

1967 ◽  
Vol 18 (2) ◽  
pp. 289 ◽  
Author(s):  
JW Randles ◽  
NC Crowley
Keyword(s):  
Mosaic Virus ◽  
South Australia ◽  
Brevicoryne Brassicae ◽  
Cauliflower Mosaic Virus ◽  
Vector Species ◽  
Aphid Vector ◽  
Brassica Crops ◽  
Significant Spread

Results are presented of 3 years of aphid trapping and 3 years of surveys of the incidence of infection with cauliflower mosaic virus in Brassica crops at two sites in South Australia. Mosaic infection has been found to be of importance only in cauliflower plantings. The incidence of the disease varied greatly at the two sites, which were located in climatically different regions. In the Adelaide Hills significant spread of the disease occurred only through the autumn months March, April, and May, and in only one of the three years did the incidence of the disease reach epidemic proportions. On the Adelaide Plains spread of the disease occurred throughout the cooler months of the year, from March till September. At both locations increasing incidence of the disease followed increases in the number of trapped aphid vector species. Results indicate that the most important vector in the field is likely to be Brevicoryne brassicae. Trials suggest that seed-bed infection with the virus can be reduced by the use of barrier crops.

scholarly journals Identification of Plant Virus Receptor Candidates in the Stylets of Their Aphid Vectors

2018 ◽  
Vol 92 (14) ◽  
Author(s):  
Craig G. Webster ◽  
Elodie Pichon ◽  
Manuella van Munster ◽  
Baptiste Monsion ◽  
Maëlle Deshoux ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Myzus Persicae ◽  
Plant Virus ◽  
Plant Viruses ◽  
Cauliflower Mosaic Virus ◽  
Insect Vectors ◽  
Virus Receptor ◽  
Content Type ◽  
Cuticular Proteins

ABSTRACTPlant viruses transmitted by insects cause tremendous losses in most important crops around the world. The identification of receptors of plant viruses within their insect vectors is a key challenge to understanding the mechanisms of transmission and offers an avenue for future alternative control strategies to limit viral spread. We here report the identification of two cuticular proteins within aphid mouthparts, and we provide experimental support for the role of one of them in the transmission of a noncirculative virus. These two proteins, named Stylin-01 and Stylin-02, belong to the RR-1 cuticular protein subfamily and are highly conserved among aphid species. Using an immunolabeling approach, they were localized in the maxillary stylets of the pea aphidAcyrthosiphon pisumand the green peach aphidMyzus persicae, in the acrostyle, an organ earlier shown to harbor receptors of a noncirculative virus. A peptide motif present at the C termini of both Stylin-01 and Stylin-02 is readily accessible all over the surface of the acrostyle. Competition forin vitrobinding to the acrostyle was observed between an antibody targeting this peptide and the helper component protein P2 ofCauliflower mosaic virus. Furthermore, silencing thestylin-01but notstylin-02gene through RNA interference decreased the efficiency ofCauliflower mosaic virustransmission byMyzus persicae. These results identify the first cuticular proteins ever reported within arthropod mouthparts and distinguish Stylin-01 as the best candidate receptor for the aphid transmission of noncirculative plant viruses.IMPORTANCEMost noncirculative plant viruses transmitted by insect vectors bind to their mouthparts. They are acquired and inoculated within seconds when insects hop from plant to plant. The receptors involved remain totally elusive due to a long-standing technical bottleneck in working with insect cuticle. Here we characterize the role of the two first cuticular proteins ever identified in arthropod mouthparts. A domain of these proteins is directly accessible at the surface of the cuticle of the acrostyle, an organ at the tip of aphid stylets. The acrostyle has been shown to bind a plant virus, and we consistently demonstrated that one of the identified proteins is involved in viral transmission. Our findings provide an approach to identify proteins in insect mouthparts and point at an unprecedented gene candidate for a plant virus receptor.

scholarly journals Lower temperature influences Cauliflower mosaic virus systemic infection

2021 ◽  
Author(s):  
Roberto Alers-Velazquez ◽  
Sushant Khandekar ◽  
Clare Muller ◽  
Jennifer Boldt ◽  
Scott Leisner
Keyword(s):  
Nucleic Acid ◽  
Actin Cytoskeleton ◽  
Mosaic Virus ◽  
Virus Particle ◽  
Systemic Infection ◽  
Cauliflower Mosaic Virus ◽  
Particle Release ◽  
Systemic Symptoms ◽  
Lower Temperature ◽  
Temperature Exposure

AbstractLower temperatures delayed development of systemic symptoms by Cauliflower mosaic virus (CaMV) in two different plant hosts. However, lower temperature exposure increased CaMV nucleic acid levels in leaves of systemically-infected turnips. Furthermore, lower temperature altered the formation of aggregates formed by the CaMV major inclusion body (IB) protein, P6. Finally, lower temperature altered the architecture of the actin cytoskeleton. These data may suggest that lower temperatures alter the actin cytoskeleton, facilitating the formation of larger IBs that hold on to their internal virions more strongly than small ones, impairing virus particle release and causing a delay in systemic infection.

scholarly journals Multiple Domains Within the Cauliflower mosaic virus Gene VI Product Interact with the Full-Length Protein

2002 ◽  
Vol 15 (10) ◽  
pp. 1050-1057 ◽  
Author(s):  
Yongzhong Li ◽  
Scott M. Leisner
Keyword(s):  
Mosaic Virus ◽  
Systemic Infection ◽  
Full Length ◽  
Cauliflower Mosaic Virus ◽  
The Other ◽  
Multifunctional Protein ◽  
Viral Propagation ◽  
Self Association ◽  
Multiple Domains ◽  
Two Hybrid

The Cauliflower mosaic virus (CaMV) gene VI product (P6) is a multifunctional protein essential for viral propagation. It is likely that at least some of these functions require P6 self-association. The work described here was performed to confirm that P6 self-associates and to identify domains involved in this interaction. Yeast two-hybrid analyses indicated that full-length P6 self-associates and that this interaction is specific. Additional analyses indicated that at least four independent domains bind to full-length P6. When a central domain (termed domain D3) was removed, these interactions were abolished. However, this deleted P6 was able to bind to the full-length wild-type protein and to isolated domain D3. Viruses lacking domain D3 were incapable of producing a systemic infection. Isolated domain D3 was capable of binding to at least two of the other domains but was unable to self-associate. This suggests that domain D3 facilitates P6 self-association by binding to the other domains but not itself. The presence of multiple domains involved in P6 self-association may help explain the ability of this protein to form the intracellular inclusions characteristic of caulimoviruses.

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