The presence of breaks in the deoxyribonucleic acid of Bacillus subtilis treated in vivo with the alkylating agent, methylmethanesulfonate

Deoxycytidylate deaminase activity and net synthesis of deoxyribonucleic acid (DNA) in vivo were found to increase at approximately the same time during the early stages of liver regeneration. However, deaminase activity in the regenerating liver remained at a high level for 1 day after DNA synthesis had slowed down again during the later stages of regeneration. The increase in deaminase activity was restricted as a result of exposure to 600 r X radiation during early regeneration, but this effect only became evident 11–16 hours after the irradiation. Irradiation on the second day after partial hepatectomy, when deaminase levels in control regenerating livers were relatively constant, failed to affect the deaminase activity immediately but did produce a 40–50% decrease in activity 11–16 hours later. Other antimitotic agents, e.g., colchicine, had little effect on deaminase activity.

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Effect of 5-bromouracil on the pattern of deoxyribonucleic acid replication in germinating Bacillus subtilis spores

Journal of Molecular Biology ◽

10.1016/0022-2836(69)90363-5 ◽

1969 ◽

Vol 43 (3) ◽

pp. 619-630 ◽

Cited By ~ 10

Author(s):

P. Nagley ◽

R.G. Wake

Keyword(s):

Bacillus Subtilis ◽

Deoxyribonucleic Acid

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In Bacillus subtilis, the Sirtuin Protein Deacetylase, Encoded by the srtN Gene (Formerly yhdZ), and Functions Encoded by the acuABC Genes Control the Activity of Acetyl Coenzyme A Synthetase

Journal of Bacteriology ◽

10.1128/jb.01674-08 ◽

2009 ◽

Vol 191 (6) ◽

pp. 1749-1755 ◽

Cited By ~ 49

Author(s):

Jeffrey G. Gardner ◽

Jorge C. Escalante-Semerena

Keyword(s):

Bacillus Subtilis ◽

Coenzyme A ◽

Acetyl Coenzyme A ◽

Acetyl Coenzyme ◽

New Information ◽

Low Concentrations ◽

Dependent Protein ◽

Protein Deacetylase

ABSTRACT This report provides in vivo evidence for the posttranslational control of the acetyl coenzyme A (Ac-CoA) synthetase (AcsA) enzyme of Bacillus subtilis by the acuA and acuC gene products. In addition, both in vivo and in vitro data presented support the conclusion that the yhdZ gene of B. subtilis encodes a NAD+-dependent protein deacetylase homologous to the yeast Sir2 protein (also known as sirtuin). On the basis of this new information, a change in gene nomenclature, from yhdZ to srtN (for sirtuin), is proposed to reflect the activity associated with the YdhZ protein. In vivo control of B. subtilis AcsA function required the combined activities of AcuC and SrtN. Inactivation of acuC or srtN resulted in slower growth and cell yield under low-acetate conditions than those of the wild-type strain, and the acuC srtN strain grew under low-acetate conditions as poorly as the acsA strain. Our interpretation of the latter result was that both deacetylases (AcuC and SrtN) are needed to maintain AcsA as active (i.e., deacetylated) so the cell can grow with low concentrations of acetate. Growth of an acuA acuC srtN strain on acetate was improved over that of the acuA + acuC srtN strain, indicating that the AcuA acetyltransferase enzyme modifies (i.e., inactivates) AcsA in vivo, a result consistent with previously reported in vitro evidence that AcsA is a substrate of AcuA.

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IN VIVO SYNTHESIS AND BREAKDOWN OF DEOXYRIBONUCLEIC ACID IN TRIBOLIUM CONFUSUM DUVAL

Canadian Journal of Biochemistry ◽

10.1139/o66-179 ◽

1966 ◽

Vol 44 (12) ◽

pp. 1571-1575 ◽

Cited By ~ 1

Author(s):

K. D. Chaudhary ◽

A. Lemonde

Keyword(s):

Dna Synthesis ◽

Growth Phase ◽

Deoxyribonucleic Acid ◽

Total Concentration ◽

Larval Growth ◽

Pupal Stage ◽

Larval Period ◽

Tribolium Confusum ◽

In Vivo Synthesis

The in vivo synthesis of deoxyribonucleic acid (DNA), as shown by the rate of incorporation of14C-thymidine, has been investigated at different stages in the life cycle of Tribolium confusum. During the larval period, a close similarity is observed between the rate of DNA synthesis and the pattern of growth. The pupal stage, which is a non-growth phase, is characterized by a cessation of DNA synthesis. During the larval growth phase, although the 3-day-old larvae have the lowest and the 13-day-old have the highest rate of DNA synthesis, the rate of DNA degradation in the older larvae is almost twice as great as that of the younger larvae. These findings are consistent with the observed total concentration of DNA of the insect at these stages.

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