HOW MANY FORMS OF THE NEWCASTLE DISEASE VIRUS P PROTEIN ARE THERE?11This work was supported by Public Health Service grant HL23588 and National Science Foundation grants PCM78–08088 and PCM81–18285. We benefited from the use of a cell culture facility supported by Public Health grant CA14733.

1984 ◽  
pp. 301-308
Author(s):  
L.E. Hightower ◽  
G.W. Smith ◽  
P.L. Collins
Keyword(s):  
Public Health ◽  
Cell Culture ◽  
Health Service ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
P Protein ◽  
National Science ◽  
A Cell ◽  
Cell Culture Facility

scholarly journals Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Azmir Arifin ◽  
Maizirwan Mel ◽  
Mohamed Ismail Abdul Karim ◽  
Aini Ideris
Keyword(s):  
Cell Culture ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
High Speed ◽  
Virus Titer ◽  
Vero Cells ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Maximum Cell

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was1.93×106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about7.95×105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that the maximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.

scholarly journals Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov

2015 ◽  
Vol 46 (3) ◽  
pp. 861-865 ◽  
Author(s):  
Sivam Visnuvinayagam ◽  
Thangavel K ◽  
Lalitha N ◽  
Malmarugan S ◽  
Kuppannan Sukumar
Keyword(s):  
Cell Culture ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Virus Strain ◽  
Newcastle Disease ◽  
Newcastle Disease Virus Strain

scholarly journals Isolation &Identification of Newcastle Disease Virus From Wild Pigeons

2020 ◽  
Vol 31 (2) ◽  
Author(s):  
Anton Sabri AL-bana
Keyword(s):  
Cell Culture ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Virus Isolation ◽  
Post Inoculation ◽  
College Of Engineering ◽  
Cytopathogenic Effect

Isolation &identification of Newcastle disease virus from wild pigeonsinhabiting University of Baghdad (Al-Khwarizmi College of engineering)where velogenic and devastating infection of birds was encountered. Chickenembryos fibroblasts (CEF) were used for virus isolation (from infectedspleen and lungs) and propagation. Viral cytopathogenic effect was noticedon infected (CEF) cell culture within (24) hr post inoculation. The virus wasidentified by heamagglutination inhibition (HI) test using reference antiNDV serum.

scholarly journals Patterns of RNA Editing in Newcastle Disease Virus Infections

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1249
Author(s):  
Archana Jadhav ◽  
Lele Zhao ◽  
Alice Ledda ◽  
Weiwei Liu ◽  
Chan Ding ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Rna Editing ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Structural Proteins ◽  
Virus Infections ◽  
Mrna Editing ◽  
P Protein

The expression of accessory non-structural proteins V and W in Newcastle disease virus (NDV) infections depends on RNA editing. These proteins are derived from frameshifts of the sequence coding for the P protein via co-transcriptional insertion of one or two guanines in the mRNA. However, a larger number of guanines can be inserted with lower frequencies. We analysed data from deep RNA sequencing of samples from in vitro and in vivo NDV infections to uncover the patterns of mRNA editing in NDV. The distribution of insertions is well described by a simple Markov model of polymerase stuttering, providing strong quantitative confirmation of the molecular process hypothesised by Kolakofsky and collaborators three decades ago. Our results suggest that the probability that the NDV polymerase would stutter is about 0.45 initially, and 0.3 for further subsequent insertions. The latter probability is approximately independent of the number of previous insertions, the host cell, and viral strain. However, in LaSota infections, we also observe deviations from the predicted V/W ratio of about 3:1 according to this model, which could be attributed to deviations from this stuttering model or to further mechanisms downregulating the abundance of W protein.

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