ALDOSTERONE—MEDIATED CHANGES IN LIPID METABOLISM IN RELATIONSHIP TO Na+ TRANSPORT IN THE AMPHIBIAN URINARY BLADDER

1976 ◽  
pp. 259-273 ◽  
Author(s):  
Howard Rasmussen ◽  
David B.P. Goodman ◽  
Eric Lien
Keyword(s):  
Lipid Metabolism ◽  
Urinary Bladder ◽  
Na Transport ◽  
Amphibian Urinary Bladder

Effect of verapamil on cytoplasmic distribution of granules and microfilaments in amphibian urinary bladder

1988 ◽  
Vol 46 ◽  
pp. 324-325
Author(s):  
A.J. Mia ◽  
L.X. Oakford ◽  
T. Yorio
Keyword(s):  
Urinary Bladder ◽  
Water Flow ◽  
Morphological Changes ◽  
Granular Cell ◽  
Cytosolic Calcium ◽  
Calcium Storage ◽  
Amphibian Urinary Bladder ◽  
Vesicular Membrane ◽  
High Concentrations ◽  
Cytoskeletal System

The amphibian urinary bladder has been used as a ‘model’ system for studies of the mechanism of action of antidiuretic hormone (ADH) in stimulating transepithelial water flow. The increase in water permeability is accompanied by morphological changes that include the stimulation of apical microvilli, mobilization of microtubules and microfilaments and vesicular membrane fusion events . It has been shown that alterations in the cytosolic calcium concentrations can inhibit ADH transmembrane water flow and induce alterations in the epithelial cell cytomorphology, including the cytoskeletal system . Recently, the subapical granules of the granular cell in the amphibian urinary bladder have been shown to contain high concentrations of calcium, and it was suggested that these cytoplasmic constituents may act as calcium storage sites for intracellular calcium homeostasis. The present study utilizes the calcium antagonist, verapamil, to examine the effect of calcium deprivation on the cytomorphological features of epithelial cells from amphibian urinary bladder, with particular emphasis on subapical granule and microfilament distribution.

Water Permeability of Amphibian Urinary Bladder

2018 ◽  
pp. 169-196
Author(s):  
Jacques Bourguet ◽  
Jacques Chevalier ◽  
Mario Parisi ◽  
Pierre Ripoche
Keyword(s):  
Urinary Bladder ◽  
Water Permeability ◽  
Amphibian Urinary Bladder

ADH-induced water permeability and particle aggregates: alteration by a synthetic estrogen

1991 ◽  
Vol 261 (1) ◽  
pp. F144-F152 ◽  
Author(s):  
G. Calamita ◽  
Y. Le Guevel ◽  
J. Bourguet
Keyword(s):  
Urinary Bladder ◽  
Water Flow ◽  
Water Permeability ◽  
Glucose Transporter ◽  
Apical Membrane ◽  
Selective Inhibition ◽  
Synthetic Estrogen ◽  
Permeability Changes ◽  
Particle Aggregates ◽  
Amphibian Urinary Bladder

In the amphibian urinary bladder, the increase in water permeability induced by antidiuretic hormone (ADH) is accompanied by the appearance of apical intramembrane particle (IMP) aggregates that are believed to contain specific channels for water. In a previous work, we have shown that 3,3'-diallyldiethylstilbestrol (DADES), a synthetic estrogen which is a blocker of the glucose transporter, also inhibits the hydrosmotic response to ADH in the bladder. Our aim in the present study was to analyze the alterations of the membrane fine structure further and to correlate them with the water permeability changes. The results point to a selective inhibition of the ADH-induced net water flow, probably due to an interference with one of the last steps of the response to the hormone. This inhibition is associated with an increase in the density of the apical IMP aggregates, which are thus probably not operational. The resting net water flow is not inhibited and, surprisingly, typical IMP aggregates are frequently observed in the apical membrane after DADES treatment. The compound also induces the appearance of unusual loose IMP clusters that can only be seen on the apical membrane of the granular cells and that share several ultrastructural similarities with the ADH-induced aggregates. These results suggest that 1) apical DADES treatment stimulates the insertion of IMP aggregates in the apical membrane of the urinary bladder and 2) DADES inhibits the ADH-induced water flow by interfering with the aggregates and thus probably by blocking the specific water channels.

Use of mass spectrometer to measure CO2 and O2 fluxes in voltage-clamped epithelia

1979 ◽  
Vol 236 (4) ◽  
pp. F413-F418
Author(s):  
S. J. Rosenthal ◽  
J. G. King ◽  
A. Essig
Keyword(s):  
Urinary Bladder ◽  
Mass Spectrometer ◽  
Ussing Chamber ◽  
Electrical Current ◽  
Toad Urinary Bladder ◽  
Co2 Efflux ◽  
Quadrupole Mass ◽  
Active Sodium ◽  
Na Transport ◽  
Made In

A quadrupole mass spectrometer was coupled to an Ussing chamber in order to evaluate rates of oxidative metabolism in voltage-clamped epithelia. Well-defined mixing characteristics of the continuously perfused chamber allowed CO2 and O2 concentrations to be related to rates of CO2 efflux, JCO2, and oxygen influx, JO2. The use of a model tissue to simulate step changes in JCO2 validated the treatment, with response within a minute. Monitoring of metabolism was facilitated by use of a desk-top computer, which evaluated JCO2 at 6-s intervals. Concurrent measurements of electrical current and JCO2 were made in the toad urinary bladder in order to relate active sodium transport to metabolism; the use of amiloride to eliminate active transport and the associated metabolism then allowed evaluation of the rates of active Na transport (JNa) and suprabasal metabolism (JsbCO2), and their ratio JNa/JsbCO2. We report the ability to resolve a 5 pmol/s change in CO2 efflux or an 11 pmol/s change in O2 influx rates.

Activation of actin-containing microfilaments by vasopressin in the amphibian urinary bladder epithelium: A fluorescent study using NBD-phallacidin

1985 ◽  
Vol 211 (3) ◽  
pp. 239-245 ◽  
Author(s):  
Walter L. Davis ◽  
Ruth Gwendolyn Jones ◽  
Phillip C. Richemont ◽  
David B. P. Goodman
Keyword(s):  
Urinary Bladder ◽  
Bladder Epithelium ◽  
Amphibian Urinary Bladder

Antidiuretic hormone-induced intramembranous alterations in mammalian collecting ducts

1978 ◽  
Vol 235 (5) ◽  
pp. F440-F443 ◽  
Author(s):  
Mehmet C. Harmanci ◽  
William A. Kachadorian ◽  
Heinz Valtin ◽  
Vincent A. DiScala
Keyword(s):  
Urinary Bladder ◽  
Antidiuretic Hormone ◽  
Collecting Duct ◽  
Freeze Fracture ◽  
Intramembranous Particle ◽  
Membrane Effect ◽  
Collecting Ducts ◽  
Amphibian Urinary Bladder ◽  
Freeze Fracture Electron Microscopy ◽  
Membrane Particle

Freeze-fracture electron microscopy had previously revealed antidiuretic hormone-induced aggregates of intramembranous particles in amphibian urinary bladder. To investigate the effects of antidiuretic hormone (ADH) in another ADH-sensitive epithelium, namely, mammalian renal collecting ducts, freeze-fracture studies were carried out in Brattleboro homozygous rats. Collecting duct luminal membranes of ADH-treated homozygotes showed intramembranous particle clusters (117 ± 17/100 μm2) that were loosely packed and that occurred on both exoplasmic (E) and protoplasmic (P) faces. Untreated, control homozygous rats had significantly less (3 ± 1/100 μm2) clusters. Changes similar to those seen in ADH-treated rats were observed in water-deprived Wistar rats. The clustered particles differed from those seen in ADH-treated amphibian urinary bladder in that the latter occurred only on the P face and were more densely packed. Nevertheless, our observations suggest a common membrane effect for ADH action that may apply in mammals and amphibia alike. freeze-fracture; Brattleboro homozygous rats; membrane particle clusters Submitted on March 6, 1978 Accepted on July 14, 1978

Aldosterone stimulates active Na+ transport in rabbit urinary bladder by both genomic and non-genomic processes

2005 ◽  
Vol 510 (3) ◽  
pp. 181-186 ◽  
Author(s):  
Timothy J. Burton ◽  
Dermot M.F. Cooper ◽  
Bryony Dunning-Davies ◽  
Dina Mansour ◽  
Nanako Masada ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Na Transport ◽  
Rabbit Urinary Bladder

scholarly journals Structural Requirements for the Action of Neurohypophyseal Hormones upon the Isolated Amphibian Urinary Bladder

1963 ◽  
Vol 46 (6) ◽  
pp. 1171-1189 ◽  
Author(s):  
Howard Rasmussen ◽  
Irving L. Schwartz ◽  
Richard Young ◽  
Julien Marc-Aurele
Keyword(s):  
Urinary Bladder ◽  
Hormone Receptor ◽  
Receptor Site ◽  
Arginine Vasotocin ◽  
Ion Concentration ◽  
Intrinsic Activity ◽  
Receptor Interaction ◽  
Hydrogen Ion Concentration ◽  
Amphibian Urinary Bladder ◽  
Ionizable Groups

The response of the isolated amphibian urinary bladder to thirty-four structural analogs of arginine vasotocin was determined in an effort to define the physiological significance of specific structural groups on the hormone molecule. All but one of the analogs tested possessed full intrinsic activity in this system but varied greatly in their affinity for the receptor site. An analysis of the effect of changes in hydrogen ion concentration upon the response of the bladder to oxytocin was performed in order to determine the number and nature of the ionizable groups involved in hormone receptor interaction. Two ionizable groups with apparent pK's of 7.1 and 7.75 were found to be important in determining the magnitude of the hormonal response. On the basis of the results it was postulated that hormone-receptor interaction can be considered a two-step process: (a) The binding or attachment of hormone to receptor site through ionic, hydrogen, and hydrophobic bonds and (b) a disulfide interchange reaction between hormonal disulfide and receptor sulfhydryl. The latter step is considered to be the reaction which initiates the chain of events leading to the observed change in permeability.

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