Cellulose Acetate Electrophoresis of Proteins of Serum, Cerebrospinal Fluid, and Urine

1970 ◽  
pp. 13-30 ◽  
Author(s):  
ALEX KAPLAN ◽  
JOHN SAVORY ◽  
WILLARD R. FAULKNER ◽  
GUILFORD G. RUDOLPH ◽  
WENDELL J. FORD ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Electrophoresis Of Proteins

Cerebrospinal Fluid Proteins: Concentration by Membrane Ultrafiltration and Fractionation by Electrophoresis on Cellulose Acetate

1970 ◽  
Vol 16 (5) ◽  
pp. 416-419 ◽  
Author(s):  
Rita M Windisch ◽  
Mark M Bracken
Keyword(s):  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Total Protein ◽  
Protein Fractions ◽  
Cellulose Acetate Electrophoresis ◽  
Mean Values ◽  
Cerebrospinal Fluid Proteins ◽  
Ultra Filtration

Abstract A membrane ultrafiltration system is described and evaluated for rapidly concentrating cerebrospinal fluid before cellulose acetate electrophoresis. Results with this system were compared with those obtained by use of vacuum ultrafiltration through a collodion sac. Mean values for the various protein fractions were determined for normal cerebrospinal fluid. The results, in percentage of total protein, after membrane and vacuum ultra-filtration concentration were, respectively: 3.8 and 5.2% prealbumin, 65.5 and 63.9% albumin, 3.6 and 3.6% ∝1-globulin, 6.8 and 6.1% arglobulin, 12.4 and 12.9% a-globulin, and 7.6 and 8.2% γ-globulin.

Concentration of Cerebrospinal Fluid Proteins and Their Fractionation by Cellulose Acetate Electrophoresis

1966 ◽  
Vol 12 (10) ◽  
pp. 717-727 ◽  
Author(s):  
Alex Kaplan ◽  
Murray Johnstone
Keyword(s):  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Convenient Method ◽  
Protein Fractions ◽  
Electrophoretic Separation ◽  
Cellulose Acetate Electrophoresis ◽  
Mean Values ◽  
Cerebrospinal Fluid Proteins ◽  
The Mean

Abstract Various technics for concentrating cerebrospinal fluid (CSF) prior to electrophoretic separation of the protein fractions were tested. Vacuum ultrafiltration through a collodion sac proved to be the most reliable and convenient method. The proteins in the concentrate were separated by cellulose acetate electrophoresis and quantitated densitometrically. The mean values for the various protein fractions in normal CSF were the following: 4.9% pre-albumin, 61.5% albumin, 4.5% α1 globulin, 6.7% α2 globulin, 13.7% β globulin, and 8.8% γ globulin.

Estimation of Serum Proteins by Electrophoresis on Cellulose Acetate

1972 ◽  
Vol 18 (12) ◽  
pp. 1541-1542 ◽  
Author(s):  
J W Keyser ◽  
G L Watkins
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Serum Proteins ◽  
Standard Procedure ◽  
Total Serum Protein ◽  
Total Serum ◽  
Cellulose Acetate Electrophoresis ◽  
Ponceau S ◽  
Reference Serum ◽  
Electrophoresis Of Proteins

Abstract We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) x total serum protein concn] and by the formula (absorbance of albumin-bound dye in test/absorbance of albumin-bound dye in a reference serum) x concn of albumin in reference serum. The ratio of values by the first and second methods ranged from 0.93 to 1.30, the first giving the higher results in cases of discrepancy. These findings confirm the limitations in accurately calculating any serum-protein fraction by the first method. The second method appears to be the more accurate.

scholarly journals CSF Electrophoresis: An Adaptation Using Cellulose Acetate for the Identification of Oligoclonal Banding

1978 ◽  
Vol 5 (3) ◽  
pp. 297-299 ◽  
Author(s):  
D.W. Paty ◽  
M. Donnelly ◽  
M.E. Bernardo
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Definite Multiple Sclerosis ◽  
Oligoclonal Banding ◽  
Clinically Definite Multiple Sclerosis

SUMMARY:An adaptation of cellulose acetate electrophoresis for studying concentrated cerebrospinal fluid is described. Two hundred and twenty-one patients have been studied, and the specificity for multiple sclerosis and sub-acute sclerosing panencephalitis is discussed. This has been positive for oligoclonal banding (OB) in 79% of patients with clinically definite multiple sclerosis.

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