scholarly journals CSF Electrophoresis: An Adaptation Using Cellulose Acetate for the Identification of Oligoclonal Banding

1978 ◽  
Vol 5 (3) ◽  
pp. 297-299 ◽  
Author(s):  
D.W. Paty ◽  
M. Donnelly ◽  
M.E. Bernardo
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Definite Multiple Sclerosis ◽  
Oligoclonal Banding ◽  
Clinically Definite Multiple Sclerosis

SUMMARY:An adaptation of cellulose acetate electrophoresis for studying concentrated cerebrospinal fluid is described. Two hundred and twenty-one patients have been studied, and the specificity for multiple sclerosis and sub-acute sclerosing panencephalitis is discussed. This has been positive for oligoclonal banding (OB) in 79% of patients with clinically definite multiple sclerosis.

scholarly journals CSF Electrophoresis in One Thousand Patients

1980 ◽  
Vol 7 (4) ◽  
pp. 275-280 ◽  
Author(s):  
G.C. Ebers ◽  
D.W. Paty
Keyword(s):  
Multiple Sclerosis ◽  
Immune Response ◽  
Cellulose Acetate ◽  
Diagnostic Criteria ◽  
Neurological Diseases ◽  
Local Immune Response ◽  
Cellulose Acetate Electrophoresis ◽  
Definite Multiple Sclerosis ◽  
Oligoclonal Banding ◽  
Clinically Definite Multiple Sclerosis

SUMMARY:Agarose and/or cellulose acetate electrophoresis was performed on the CSF of one thousand patients. In patients with clinically definite multiple sclerosis (MS) (N = 267) 92.8% had oligoclonal banding (O.B.). In patients with possible MS (N - 283) O.B. was present in 31.1%. In patients with other neurological diseases (N = 450) O.B. was present in 8% (N = 36). Nineteen non-MS patients with positive O.B. had serum bands or disorders known to be associated with local immune response. The remaining 17 patients had no explanation for the oligoclonal banding. In the majority of these MS had not been a diagnostic consideration.CSF electrophoresis is the single most reliable laboratory lest in multiple sclerosis and deserves incorporation into the diagnostic criteria for the disease.

Radiologically isolated syndrome: targeting miRNAs as prognostic biomarkers

Epigenomics ◽  
2020 ◽  
Vol 12 (23) ◽  
pp. 2065-2076
Author(s):  
María Muñoz-San Martín ◽  
Sandra Torras ◽  
René Robles-Cedeño ◽  
Maria Buxó ◽  
Imma Gomez ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Prognostic Factors ◽  
Prognostic Biomarkers ◽  
Circulating Mirnas ◽  
Radiologically Isolated Syndrome ◽  
Definite Multiple Sclerosis ◽  
Clinically Definite Multiple Sclerosis ◽  
Specific Profile

Aim: Some clinical and biological characteristics have been described as prognostic factors for clinical conversion into clinically definite multiple sclerosis in radiologically isolated syndrome (RIS) population. The aim of this study was to assess signatures of circulating miRNAs in those patients according to their conversion status after 5 years of follow-up. Patients & methods: OpenArray plates assessing 216 miRNA candidates were run in 15 RIS patients, and their relative abundances were analyzed. Results: A specific profile of deregulated circulating miRNAs (miR-144-3p, miR-448 and miR-653-3p in cerebrospinal fluid and miR-142-3p, miR-338-3p, miR-363-3p, miR-374b-5p, miR-424-5p, miR-483-3p in plasma) differentiated individuals who remained as RIS after 5 years of follow-up. Conclusion: Circulating miRNAs might be used as prognostic biomarkers for RIS patients.

Differentiating patterns of oligoclonal banding in the cerebrospinal fluid improves diagnostic utility for multiple sclerosis

Pathology ◽  
2012 ◽  
Vol 44 (3) ◽  
pp. 248-250 ◽  
Author(s):  
Ming-Wei Lin ◽  
Dan Suan ◽  
Kerry Lenton ◽  
Tony Henniker ◽  
Therese Burke ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Diagnostic Utility ◽  
Oligoclonal Banding

Prospective study of multiple sclerosis with early onset

2002 ◽  
Vol 8 (2) ◽  
pp. 115-118 ◽  
Author(s):  
A Ghezzi ◽  
C Pozzilli ◽  
M Liguori ◽  
M G Marrosu ◽  
N Milani ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
First Year ◽  
Hormonal Changes ◽  
Disability Status ◽  
Definite Multiple Sclerosis ◽  
Relapsing Remitting ◽  
Clinically Definite Multiple Sclerosis ◽  
The Mean ◽  
And Gender

Fifty-four subjects (36 females and 18 males) affected by clinically definite multiple sclerosis (MS) and with onset of the disease at 15 years of age or before were prospectively studied in five Italian MS centres. Female/male ratio was 4.7 in subjects with age ≥12 years, suggesting a role of hormonal changes in triggering MS onset. The mean follow-up duration was 10.9-5.6 years. The functional systems more frequently involved at onset were the pyramidal and brainstem (both in 28% of cases). The onset was monosymptomatic in 31 subjects (57%). The course was relapsing-remitting in 39 subjects (72%) and relapsing-progressive in 15 (28%). Disability was assessed by the Expanded Disability Status Scale (EDSS): the mean score after 8 years of follow up was 3.5 (-2.5). The score was <4 in 68% of cases, between 4 and 6 in 8% of cases, > 6 in 24% of cases. Disability after 8 years was highly predicted by disability in the first year (p=0.008). There was a tendency to a worse prognosis in relation to the number of relapses in the first 2 years (p=0.08). The outcome was not influenced by the characteristics of symptoms at onset, age and gender.

Cellulose Acetate Electrophoresis of Proteins of Serum, Cerebrospinal Fluid, and Urine

1970 ◽  
pp. 13-30 ◽  
Author(s):  
ALEX KAPLAN ◽  
JOHN SAVORY ◽  
WILLARD R. FAULKNER ◽  
GUILFORD G. RUDOLPH ◽  
WENDELL J. FORD ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Electrophoresis Of Proteins

The cerebrospinal fluid immunoglobulin G index as a diagnostic aid in multiple sclerosis: a Bayesian approach.

1982 ◽  
Vol 28 (2) ◽  
pp. 354-355 ◽  
Author(s):  
E A Hische ◽  
H J van der Helm ◽  
H K van Walbeek
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Immunoglobulin G ◽  
Graphical Representation ◽  
Likelihood Ratios ◽  
Serum Samples ◽  
Predictive Values ◽  
Definite Multiple Sclerosis ◽  
Igg Index ◽  
Diagnostic Usefulness

Abstract Having determined immunoglobulin G (IgG) and albumin concentrations in 1100 cerebrospinal fluid and serum samples, we calculated the IgG index. Likelihood ratios for multiple sclerosis were calculated by using a training set consisting of 100 patients with definite multiple sclerosis and one consisting of 97 patients suffering from diseases from which multiple sclerosis must be differentiated. Predictive values for multiple sclerosis, given different values for the IgG index, are given in a graphical representation of Bayes' theorem. We conclude that this approach increases the diagnostic usefulness of the IgG index for the diagnosis of multiple sclerosis.

Cerebrospinal Fluid Proteins: Concentration by Membrane Ultrafiltration and Fractionation by Electrophoresis on Cellulose Acetate

1970 ◽  
Vol 16 (5) ◽  
pp. 416-419 ◽  
Author(s):  
Rita M Windisch ◽  
Mark M Bracken
Keyword(s):  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Total Protein ◽  
Protein Fractions ◽  
Cellulose Acetate Electrophoresis ◽  
Mean Values ◽  
Cerebrospinal Fluid Proteins ◽  
Ultra Filtration

Abstract A membrane ultrafiltration system is described and evaluated for rapidly concentrating cerebrospinal fluid before cellulose acetate electrophoresis. Results with this system were compared with those obtained by use of vacuum ultrafiltration through a collodion sac. Mean values for the various protein fractions were determined for normal cerebrospinal fluid. The results, in percentage of total protein, after membrane and vacuum ultra-filtration concentration were, respectively: 3.8 and 5.2% prealbumin, 65.5 and 63.9% albumin, 3.6 and 3.6% ∝1-globulin, 6.8 and 6.1% arglobulin, 12.4 and 12.9% a-globulin, and 7.6 and 8.2% γ-globulin.

scholarly journals Detection and classification of paraproteins by capillary immunofixation/subtraction

1998 ◽  
Vol 44 (4) ◽  
pp. 760-764 ◽  
Author(s):  
Xavier Bossuyt ◽  
Ann Bogaerts ◽  
Gilberte Schiettekatte ◽  
Norbert Blanckaert
Keyword(s):  
Capillary Electrophoresis ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Detection And Identification ◽  
Agarose Electrophoresis ◽  
Oligoclonal Banding ◽  
Capillary Zone ◽  
Electrophoresis System ◽  
Selection Of

Abstract A selection of 58 specimens with a monoclonal component identified by immunoelectrophoresis and/or immunofixation was analyzed with the immunosubtraction procedure on the Paragon 2000 capillary electrophoresis system. The capillary system detected 93% of the paraproteins and, using immunosubtraction, correctly identified 91% of the paraproteins. Paraproteins that were detected by immunofixation and/or immunoelectrophoresis but not by capillary electrophoresis were also missed by agarose electrophoresis and cellulose acetate electrophoresis. Cellulose acetate electrophoresis was the least sensitive method for detection of paraproteins. Only 74% of the monoclonal components were detected by this technique, whereas 86% were revealed by agarose electrophoresis. In addition to monoclonal paraproteins, we also studied biclonal paraproteins and oligoclonal banding. Capillary electrophoresis and immunosubtraction correctly detected and identified three specimens containing biclonal paraproteins. In one specimen, capillary zone electrophoresis detected only one band, whereas agarose gel electrophoresis detected two bands. The sensitivity for detection and identification of oligoclonal banding by capillary electrophoresis was inferior to immunofixation.

scholarly journals Smoking at time of CIS increases the risk of clinically definite multiple sclerosis

2018 ◽  
Vol 265 (5) ◽  
pp. 1010-1015 ◽  
Author(s):  
Roos M. van der Vuurst de Vries ◽  
Julia Y. Mescheriakova ◽  
Tessel F. Runia ◽  
Theodora A. M. Siepman ◽  
Beatrijs H. A. Wokke ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Definite Multiple Sclerosis ◽  
Clinically Definite Multiple Sclerosis
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