Monoclonal immunoglobulins demonstrable in cerebrospinal fluid by use of cellulose acetate electrophoresis.

1980 ◽  
Vol 26 (13) ◽  
pp. 1917-1918 ◽  
Author(s):  
H J van der Helm ◽  
E A Hische ◽  
H K van Walbeek
Keyword(s):  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Monoclonal Immunoglobulins

Cellulose Acetate Electrophoresis of Proteins of Serum, Cerebrospinal Fluid, and Urine

1970 ◽  
pp. 13-30 ◽  
Author(s):  
ALEX KAPLAN ◽  
JOHN SAVORY ◽  
WILLARD R. FAULKNER ◽  
GUILFORD G. RUDOLPH ◽  
WENDELL J. FORD ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Electrophoresis Of Proteins

Cerebrospinal Fluid Proteins: Concentration by Membrane Ultrafiltration and Fractionation by Electrophoresis on Cellulose Acetate

1970 ◽  
Vol 16 (5) ◽  
pp. 416-419 ◽  
Author(s):  
Rita M Windisch ◽  
Mark M Bracken
Keyword(s):  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Total Protein ◽  
Protein Fractions ◽  
Cellulose Acetate Electrophoresis ◽  
Mean Values ◽  
Cerebrospinal Fluid Proteins ◽  
Ultra Filtration

Abstract A membrane ultrafiltration system is described and evaluated for rapidly concentrating cerebrospinal fluid before cellulose acetate electrophoresis. Results with this system were compared with those obtained by use of vacuum ultrafiltration through a collodion sac. Mean values for the various protein fractions were determined for normal cerebrospinal fluid. The results, in percentage of total protein, after membrane and vacuum ultra-filtration concentration were, respectively: 3.8 and 5.2% prealbumin, 65.5 and 63.9% albumin, 3.6 and 3.6% ∝1-globulin, 6.8 and 6.1% arglobulin, 12.4 and 12.9% a-globulin, and 7.6 and 8.2% γ-globulin.

Concentration of Cerebrospinal Fluid Proteins and Their Fractionation by Cellulose Acetate Electrophoresis

1966 ◽  
Vol 12 (10) ◽  
pp. 717-727 ◽  
Author(s):  
Alex Kaplan ◽  
Murray Johnstone
Keyword(s):  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Convenient Method ◽  
Protein Fractions ◽  
Electrophoretic Separation ◽  
Cellulose Acetate Electrophoresis ◽  
Mean Values ◽  
Cerebrospinal Fluid Proteins ◽  
The Mean

Abstract Various technics for concentrating cerebrospinal fluid (CSF) prior to electrophoretic separation of the protein fractions were tested. Vacuum ultrafiltration through a collodion sac proved to be the most reliable and convenient method. The proteins in the concentrate were separated by cellulose acetate electrophoresis and quantitated densitometrically. The mean values for the various protein fractions in normal CSF were the following: 4.9% pre-albumin, 61.5% albumin, 4.5% α1 globulin, 6.7% α2 globulin, 13.7% β globulin, and 8.8% γ globulin.

scholarly journals CSF Electrophoresis: An Adaptation Using Cellulose Acetate for the Identification of Oligoclonal Banding

1978 ◽  
Vol 5 (3) ◽  
pp. 297-299 ◽  
Author(s):  
D.W. Paty ◽  
M. Donnelly ◽  
M.E. Bernardo
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Definite Multiple Sclerosis ◽  
Oligoclonal Banding ◽  
Clinically Definite Multiple Sclerosis

SUMMARY:An adaptation of cellulose acetate electrophoresis for studying concentrated cerebrospinal fluid is described. Two hundred and twenty-one patients have been studied, and the specificity for multiple sclerosis and sub-acute sclerosing panencephalitis is discussed. This has been positive for oligoclonal banding (OB) in 79% of patients with clinically definite multiple sclerosis.

Usefulness of Cation Exchange High Performance Liquid Chromatography as a Testing Procedure

PEDIATRICS ◽  
1989 ◽  
Vol 83 (5) ◽  
pp. 849-851
Author(s):  
Titus H. J. Huisman
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cation Exchange ◽  
Cellulose Acetate ◽  
Isoelectric Focusing ◽  
High Performance ◽  
Testing Procedure ◽  
Cellulose Acetate Electrophoresis ◽  
Blood Samples ◽  
Testing Procedures

Testing of cord blood or newborn blood samples for hemoglobin abnormalities should include clinically important hemoglobinopathies other than sickle cell anemia (SS), such as SC, SD, SO, S-β- thalassemia (thal), EE, SE, and α-thal, and should place the quality of the testing procedures (ie, accuracy of diagnosis) above quantity (ie, number of samples tested over a given period). There is no single method available that is suitable for the identification of each of the numerous abnormalities; thus, at least two, and often more than two, procedures must be used to reach a definitive diagnosis. For this reason, blood samples collected in vacutainers with ethylenediaminetetraacetic acid as anticoagulant are preferred to those collected on filter papers. The latter approach also has the disadvantage that, under a less than optimal transport system, hemoglobin is readily modified (oxidation, glycosylation, protein-protein interaction), producting extra bands or peaks in electrophoretic or chromatographic separations that interfere with an appropriate identification of various genetically determined hemoglobin variants. In our laboratories, in which hemoglobin identification has been routine for more than 25 years, we consider the following procedures acceptable primary testing methods: starch gel electrophoresis at pH 8.9, cellulose acetate electrophoresis at pH 8.5 to 8.9, isoelectric focusing, and fast cation exchange high performance liquid chromatography (HPLC). The following five methods are excellent confirmatory testing procedures: citrate agar electrophoresis at pH 6.1, cation or anion exchange macrochromatography, isoelectric focusing, cation exchange HPLC, and immunologic procedures. Combinations of these techniques will often lead to acceptable data, and the general approach followed in our institute is given in Fig 1. Cellulose acetate electrophoresis at alkaline pH is still the primary testing procedure, and citrate agar electrophoresis at pH 6.1 and micro-HPLC procedures are the main confirmatory methods.

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