Characterization of Stem Cells Using Reverse Transcriptase Polymerase Chain Reaction

2007 ◽  
pp. 127-148
Author(s):  
Shengwen Li ◽  
Philip H. Schwartz ◽  
Ivan Kirov ◽  
Henry J. Klassen
Keyword(s):  
Stem Cells ◽  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Chain Reaction ◽  
Polymerase Chain

Detection by reverse transcriptase-polymerase chain reaction and molecular characterization of subtype B avian metapneumovirus isolated in Brazil

2007 ◽  
Vol 36 (5) ◽  
pp. 383-387 ◽  
Author(s):  
Jorge Luis Chacón ◽  
Paulo E. Brandão ◽  
Marcos Buim ◽  
Laura Villarreal ◽  
Antonio J. Piantino Ferreira
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Molecular Characterization ◽  
Chain Reaction ◽  
Avian Metapneumovirus ◽  
Subtype B ◽  
Polymerase Chain

scholarly journals G and P rotavirus genotypes in stool samples from children in Teresina, State of Piauí

2007 ◽  
Vol 40 (4) ◽  
pp. 381-384 ◽  
Author(s):  
Carla Isabel Macedo ◽  
Alice Christofoletti ◽  
Veridiana Munford ◽  
Maria Lúcia Rácz
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Molecular Characterization ◽  
Interspecies Transmission ◽  
Chain Reaction ◽  
Stool Samples ◽  
Stool Specimens ◽  
Polymerase Chain ◽  
Gene Reassortment

A total of 123 stool specimens collected in Teresina, Piauí between 1994 and 1996, from 0 to 2-year-old children with diarrhea, were used for this study. Molecular characterization of the G and P rotavirus genotypes was performed using the reverse transcriptase polymerase chain reaction. The following results were obtained for the P genotypes: P[8] (17. 1%), P[1] (4. 9%), P[4] (3. 3%), P[6, M37] (2. 4%) and mixtures (27. 6%). The P[1]+P[8] mixture was found in 19. 5% of the samples. For the G genotypes, the results were: G1 (25. 2%), G5 (13. 8%), G2 (2. 5%), G4 (2. 5%), G9 (0. 8%) and mixtures (41. 5%). G1+G5 was the mixture most frequently found (12. 1%). Our results showed unusual combinations such as P[1]G5 and P[1]+P[8]G5. The high percentage of mixtures and unusual combinations containing mixtures of human and animal rotavirus genotypes strongly suggests the possibility of gene reassortment and interspecies transmission.

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