VARIABLES IN THE GAS CHROMATOGRAPHIC PROCESS

1997 ◽  
pp. 114-154
Author(s):  
WALTER JENNINGS ◽  
ERIC MITTLEFEHLDT ◽  
PHILIP STREMPLE
Keyword(s):  
Chromatographic Process

scholarly journals Relationship between Stationary and Mobile Phase Composition and its Influence on the Retention Time of Some Analytes in Gas Chromatography

2000 ◽  
Vol 18 (4) ◽  
pp. 323-331
Author(s):  
R. Nasuto
Keyword(s):  
Gas Chromatography ◽  
Mobile Phase ◽  
Hydrogen Gas ◽  
Gas Mixture ◽  
Frontal Analysis ◽  
Mobile Phase Composition ◽  
Column Packing ◽  
Chromatographic Process ◽  
Mobile Phase Modifier ◽  
Methanol Vapour

A binary methanol vapour/hydrogen gas mixture has been used as the mobile phase in gas chromatography. Through the use of modified frontal analysis (undertaken just before measurements of the retention of the tested analytes), it has been possible to determine the adsorption isotherm of the mobile phase modifier (methanol) under typical conditions for a chromatographic process. It was found that adsorption of the mobile phase modifier on the column packing surface caused a decrease in the retention times of all the analytes tested. Furthermore, as a result of such adsorption, an increase in the degree of hydrophobization of the column packing surface also occurred, leading to a decrease of the selectivity of the packing.

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