A functional dual-coated (FDC) microtiter plate method to replace the botulinum toxin LD50 test

A microtiter plate format previously reported for the biochemical characterization of microorganisms was modified by adding agar to all of the different test media instead of using a combination of liquid and solid media. This modification, termed the Modified Microtiter Plate procedure (MMP), offered the same advantages of the original method (labor saving, inexpensive, and custom designed for special needs) as well as having the added advantages of longer term storage prior to use and ease of handling. In this study, 60 bacterial isolates (both Listeria suspects and known Listeria cultures) were biotyped using the MMP protocol and compared to results obtained using the classical tube-based US-FDA recommended protocol as the standard identification method and the BBL-Minitek system.

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MICROTITER PLATE METHOD FOR IDENTIFICATION OF PTP1B PHOSPHATASE INHIBITORS

Journal of Volgograd State Medical University ◽

10.19163/1994-9480-2019-2(70)-81-83 ◽

2019 ◽

Vol 70 (2) ◽

pp. 81-83

Author(s):

D.A. Babkov ◽

◽

E.V. Sokolova ◽

A.A. Brigadirova ◽

O.Yu. Zakharyashcheva ◽

...

Keyword(s):

Microtiter Plate ◽

Plate Method ◽

Phosphatase Inhibitors

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Antibiofilm Activity of Methanol Extract of Rumex dentatus Against Pseudomonas aeruginosa

Biosis:Biological Systems ◽

10.37819/biosis.001.01.0044 ◽

2020 ◽

Vol 1 (1) ◽

pp. 25-32

Author(s):

Maryam Pezeshki Najafabadi ◽

Maryam Mohammadi-Sichani ◽

Mohammad Javad Kazemi ◽

Mohammad Sadegh Shirsalimian ◽

Majid Tavakoli

Keyword(s):

Pseudomonas Aeruginosa ◽

Biofilm Formation ◽

Inhibitory Concentration ◽

Methanol Extract ◽

Microtiter Plate ◽

Extracellular Polysaccharides ◽

Antibiofilm Activity ◽

Plate Method ◽

Hospital Acquired Infections ◽

Hospital Acquired

Biofilm formation of Pseudomonas aeruginosa makes up a sizeable proportion of hospital-acquired infections, because bacteria in biofilms can resist antibiotic treatment. The extracellular polymeric substance of P. aeruginosa biofilm is an imprecise collection of extracellular polysaccharides, proteins and microbial cells. Rumex dentatus belongs to polygonaceae family. This family can be found in Middle East. The aim of this present study was to assess the effect of various concentrations of methanol extract of Rumex dentatus on biofilm formation of Pseudomonas aeruginosa after 48 h and 72 h. In this experimental study we collected Rumex dentatus from Khoramabad, Iran. The working extracts were 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.95, 0.97 and 0.48 mg/ml. We used microtiter plate method to grow P. aeruginosa biofilm and assess the antibiofilm activity of plant extract. The composition of methanol extract obtained from Rumex dentatus was studied by gas chromatography. The minimum biofilm inhibitory concentration (MBIC) for P. aeruginosa found to be 250 mg/ml. GC-MS analyses indicated that these fractions contained a variety of compounds including Bicyclo (3.1.1) heptan- 3 -one, 2, 6, 6- trimethyl, Bicyclo (3.1.1) heptan, 6, 6- dimethyl and Eucalyptol. There were consequential correlations between antibiofilm activity and the concentration of extracts after 48 and 72 h.

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