Highly sensitive fluorescence assay of T4 polynucleotide kinase activity and inhibition via enzyme-assisted signal amplification

2014 ◽  
Vol 464 ◽  
pp. 63-69 ◽  
Author(s):  
Mangjuan Tao ◽  
Jing Zhang ◽  
Yan Jin ◽  
Baoxin Li
Keyword(s):  
Signal Amplification ◽  
Kinase Activity ◽  
Fluorescence Assay ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

Sensitive and label-free T4 polynucleotide kinase/phosphatase detection based on poly(thymine)-templated copper nanoparticles coupled with nicking enzyme-assisted signal amplification

2016 ◽  
Vol 8 (13) ◽  
pp. 2831-2836 ◽  
Author(s):  
Jia Ge ◽  
Lin Zhang ◽  
Zhen-Zhen Dong ◽  
Qi-Yong Cai ◽  
Zhao-Hui Li
Keyword(s):  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Label Free ◽  
Free T4 ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
Nicking Enzyme ◽  
T4 Polynucleotide Kinase

A novel label-free biosensor for highly sensitive sensing of the activity as well as inhibition of T4 PNKP based on poly T-templated fluorescent CuNPs and nicking enzyme-assisted signal amplification.

A highly sensitive fluorescence assay for methyltransferase activity by exonuclease-aided signal amplification

The Analyst ◽  
2015 ◽  
Vol 140 (13) ◽  
pp. 4636-4641 ◽  
Author(s):  
Feng Tang ◽  
Xi-Wen Xing ◽  
Jie-Mei Chu ◽  
Quan Yuan ◽  
Xiang Zhou ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Fluorescence Assay ◽  
Methyltransferase Activity ◽  
Highly Sensitive ◽  
Dna Adenine Methyltransferase

A highly sensitive fluorescence assay for DNA adenine methyltransferase activity was developed using exonuclease-aided signal amplification.

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