DNAzyme-driven DNA walker biosensor for amplified electrochemical detection of T4 polynucleotide kinase activity and inhibition

2020 ◽  
Vol 874 ◽  
pp. 114470
Author(s):  
Jingxiu Mao ◽  
Xin Chen ◽  
Huahuang Xu ◽  
Xueqin Xu
Keyword(s):  
Electrochemical Detection ◽  
Kinase Activity ◽  
Dna Walker ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

Ferrocene-functionalized SWCNT for electrochemical detection of T4 polynucleotide kinase activity

2012 ◽  
Vol 32 (1) ◽  
pp. 213-218 ◽  
Author(s):  
Yonghong Wang ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
Xiaoqi Ni ◽  
Jing Su ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Kinase Activity ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

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