Sensitive fluorescence sensing of T4 polynucleotide kinase activity and inhibition based on DNA/polydopamine nanospheres platform

Talanta ◽  
2018 ◽  
Vol 180 ◽  
pp. 271-276 ◽  
Author(s):  
Yao Cen ◽  
Wen-Jing Deng ◽  
Ru-Qin Yu ◽  
Xia Chu
Keyword(s):  
Kinase Activity ◽  
Fluorescence Sensing ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

2014 ◽  
Vol 50 (40) ◽  
pp. 5313-5315 ◽  
Author(s):  
Hong-Zhang He ◽  
Ka-Ho Leung ◽  
Wei Wang ◽  
Daniel Shiu-Hin Chan ◽  
Chung-Hang Leung ◽  
...  
Keyword(s):  
Kinase Activity ◽  
Label Free ◽  
Polynucleotide Kinase ◽  
G Quadruplex ◽  
T4 Polynucleotide Kinase

Detection of T4 polynucleotide kinase based on a MnO2nanosheet-3,3′,5,5′-tetramethylbenzidine (TMB) colorimetric system

2016 ◽  
Vol 8 (20) ◽  
pp. 4119-4126 ◽  
Author(s):  
Lin Lin ◽  
Dongmin Shi ◽  
Quanfa Li ◽  
Guangfeng Wang ◽  
Xiaojun Zhang
Keyword(s):  
Nucleic Acid ◽  
Kinase Activity ◽  
Acid Metabolism ◽  
Nucleic Acid Metabolism ◽  
Colorimetric System ◽  
System P ◽  
Polynucleotide Kinase ◽  
Nucleotide Kinase ◽  
T4 Polynucleotide Kinase

Determination of nucleotide kinase activity is valuable due to its importance in regulating nucleic acid metabolism.

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