Determination of DNase activity by degradation of ethidium bromide–DNA complexes using a fluorescence plate reader

2015 ◽  
Vol 471 ◽  
pp. 73-79 ◽  
Author(s):  
Benjamin Vogel ◽  
Stefan Frantz
Keyword(s):  
Ethidium Bromide ◽  
Dnase Activity ◽  
Dna Complexes ◽  
Plate Reader ◽  
Fluorescence Plate Reader

scholarly journals An Efficient and Simultaneous Analysis of Caffeine and Paracetamol in Pharmaceutical Formulations Using TLC with a Fluorescence Plate Reader

2011 ◽  
Vol 94 (4) ◽  
pp. 1094-1099 ◽  
Author(s):  
Hossein Tavallali ◽  
S Faraneh Zareiyan J. ◽  
Maryam Naghian
Keyword(s):  
Ethyl Acetate ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
Detection Limits ◽  
Pharmaceutical Formulations ◽  
Fluorescent Indicator ◽  
Solvent Type ◽  
Plate Reader ◽  
Fluorescence Plate Reader

Abstract A simple, rapid, and effcient method using TLC with a fluorescence plate reader has been described for simultaneous determination of caffeine and paracetamol. Determination was carried out using the fluorescencequenching action of caffeine and paracetamol on a TLC plate with a fluorescent indicator at λ ex = 254 nm in the linear ranges of 0.2–1.9 and 0.03–1.5 µg/L, respectively. Separation of caffeine and paracetamol were performed on the TLC plate, and the best results were obtained using the optimized mobile phase n-hexane–ethyl acetate–ethanol (2.5 + 1.5 + 0.4, v/v). Some important parameters, such as solvent type and ratio of the mobile phase, the presence of other components, and instrumental parameters, were studied. Caffeine and paracetamol detection limits were 0.025 and 0.032 µg/L, and RSD values for 0.6 µg/L caffeine and 0.06 µg/L paracetamol (n = 5) were 1.93 and 2.06%, respectively. Using this technique, some pharmaceuticals containing caffeine and paracetamol were analyzed with satisfactory results.

scholarly journals Determination of Growth Rate of Microorganisms in Broth from Oxygen-Sensitive Fluorescence Plate Reader Measurements

BioTechniques ◽  
2002 ◽  
Vol 32 (3) ◽  
pp. 684-689 ◽  
Author(s):  
David T. Stitt ◽  
Mandar S. Nagar ◽  
Tariq A. Haq ◽  
Mark R. Timmins
Keyword(s):  
Growth Rate ◽  
Plate Reader ◽  
Oxygen Sensitive ◽  
Fluorescence Plate Reader

Routine determination of enzyme kinetics using Plate reader

1991 ◽  
Vol 38 (4) ◽  
pp. 434-437 ◽  
Author(s):  
F. Galgani ◽  
Y. Cadiou ◽  
G. Bocquene
Keyword(s):  
Enzyme Kinetics ◽  
Plate Reader

scholarly journals Fluorometric Determination of Deoxyribonucleic Acid in Bacteria with Ethidium Bromide

1972 ◽  
Vol 24 (2) ◽  
pp. 179-183 ◽  
Author(s):  
J. A. Donkersloot ◽  
S. A. Robrish ◽  
M. I. Krichevsky
Keyword(s):  
Ethidium Bromide ◽  
Deoxyribonucleic Acid ◽  
Fluorometric Determination

Use of a Fluorescence Plate Reader for Measuring Kinetic Parameters with Inner Filter Effect Correction

1999 ◽  
Vol 267 (2) ◽  
pp. 331-335 ◽  
Author(s):  
Yaya Liu ◽  
Warren Kati ◽  
Chih-Ming Chen ◽  
Rakesh Tripathi ◽  
Akhter Molla ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Inner Filter Effect ◽  
Filter Effect ◽  
Plate Reader ◽  
Fluorescence Plate Reader
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