Determination of Growth Rate of Microorganisms in Broth from Oxygen-Sensitive Fluorescence Plate Reader Measurements

Abstract A simple, rapid, and effcient method using TLC with a ﬂuorescence plate reader has been described for simultaneous determination of caffeine and paracetamol. Determination was carried out using the ﬂuorescencequenching action of caffeine and paracetamol on a TLC plate with a ﬂuorescent indicator at λ ex = 254 nm in the linear ranges of 0.2–1.9 and 0.03–1.5 µg/L, respectively. Separation of caffeine and paracetamol were performed on the TLC plate, and the best results were obtained using the optimized mobile phase n-hexane–ethyl acetate–ethanol (2.5 + 1.5 + 0.4, v/v). Some important parameters, such as solvent type and ratio of the mobile phase, the presence of other components, and instrumental parameters, were studied. Caffeine and paracetamol detection limits were 0.025 and 0.032 µg/L, and RSD values for 0.6 µg/L caffeine and 0.06 µg/L paracetamol (n = 5) were 1.93 and 2.06%, respectively. Using this technique, some pharmaceuticals containing caffeine and paracetamol were analyzed with satisfactory results.

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Determination of the Growth Rate of Hydrothermally Synthesised ZnO2 Crystallites

Journal of Nano Research ◽

10.4028/www.scientific.net/jnanor.50.41 ◽

2017 ◽

Vol 50 ◽

pp. 41-47 ◽

Cited By ~ 1

Author(s):

Martin Egblewogbe ◽

Garu Gebreyesus ◽

Samuel A. Atarah

Keyword(s):

Growth Rate ◽

Zinc Acetate Dihydrate ◽

Hydrothermal Process ◽

Growth Rate Constant ◽

Acetate Dihydrate ◽

X Ray ◽

X Ray Scattering ◽

Transmission Electron ◽

Zinc Peroxide

Powders consisting of nanoparticles of zinc peroxide were prepared via a simple hydrothermal process using zinc acetate dihydrate and hydrogen peroxide precursors. The size of the crystallites was determined using x-ray powder diffraction. Over a period of 5 hours the crystallite radius increased from 4 nm – 9 nm at a temperature of 68 °C ± 5 °C, with growth rate constant of 0.23 nm3 min−1 calculated using the Lifshitz, Slyozov, and Wagner model. The powders were further characterised with High Resolution Transmission Electron Microscopy, Energy Dispersive X-ray analysis, and Small Angle X-ray Scattering, showing well-crystallised ZnO2 nanoparticles.

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Routine determination of enzyme kinetics using Plate reader

Biotechnology and Bioengineering ◽

10.1002/bit.260380413 ◽

1991 ◽

Vol 38 (4) ◽

pp. 434-437 ◽

Cited By ~ 6

Author(s):

F. Galgani ◽

Y. Cadiou ◽

G. Bocquene

Keyword(s):

Enzyme Kinetics ◽

Plate Reader

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DETERMINATION OF THE SPECIFIC GROWTH RATE ON DEGRADATION OF CASSAVA SEWAGE USING MICHAELIS-MENTEN EQUATION

Nigerian Journal of Technology ◽

10.4314/njt.v35i3.27 ◽

2016 ◽

Vol 35 (3) ◽

pp. 669

Author(s):

IM Jideofor

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Specific Growth

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Determination of collected porcini and chanterelle quantities depending on different climate change scenarios

Glasnik Sumarskog fakulteta ◽

10.2298/gsf1613077r ◽

2016 ◽

pp. 77-98

Author(s):

Nenad Rankovic ◽

Jelena Nedeljkovic ◽

Zoran Poduska ◽

Dragan Nonic

Keyword(s):

Climate Change ◽

Growth Rate ◽

Regression Models ◽

The Other ◽

Climate Change Scenarios ◽

Exponential Growth Rate ◽

Scientific Methods ◽

Exponential Regression ◽

Modelling Methods

This study examines the influence of some climate elements on the collected quantities of two commercially most significant types of mushrooms in Serbia (porcini and chanterelle). The main objective of the research is to determine the extent of the collected quantity of porcini and chanterelle, which can be expected in different scenarios of climate change (?1Bmin, ?1Bmax, A2min ? A2max), based on forecasts of temperature and rainfall changes. The general (dialectical) and specific (modelling methods) are used in the research, as well as the classical scientific methods of reasoning. The calculation of the average annual exponential growth rate (IS) was carried out by forming exponential regression models of the trend of porcini and chanterelle collected quantities. In the research it was found that, according to the data related to the period up to 2014, one can expect a decrease in the movement of both porcini and chanterelle IS, and thus a decrease in the collected quantities. On the other hand, according to the data related to the period up to 2040, in both cases one can expect some fluctuation (increase and decrease) in the movement of IS. According to the data related to periods after 2041 (especially for the period until 2100), in both cases, one can expect a decrease in the collected quantities, as a result of changes in T and P, caused by the assumed climate change.

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