scholarly journals An Efficient and Simultaneous Analysis of Caffeine and Paracetamol in Pharmaceutical Formulations Using TLC with a Fluorescence Plate Reader

2011 ◽  
Vol 94 (4) ◽  
pp. 1094-1099 ◽  
Author(s):  
Hossein Tavallali ◽  
S Faraneh Zareiyan J. ◽  
Maryam Naghian
Keyword(s):  
Ethyl Acetate ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
Detection Limits ◽  
Pharmaceutical Formulations ◽  
Fluorescent Indicator ◽  
Solvent Type ◽  
Plate Reader ◽  
Fluorescence Plate Reader

Abstract A simple, rapid, and effcient method using TLC with a fluorescence plate reader has been described for simultaneous determination of caffeine and paracetamol. Determination was carried out using the fluorescencequenching action of caffeine and paracetamol on a TLC plate with a fluorescent indicator at λ ex = 254 nm in the linear ranges of 0.2–1.9 and 0.03–1.5 µg/L, respectively. Separation of caffeine and paracetamol were performed on the TLC plate, and the best results were obtained using the optimized mobile phase n-hexane–ethyl acetate–ethanol (2.5 + 1.5 + 0.4, v/v). Some important parameters, such as solvent type and ratio of the mobile phase, the presence of other components, and instrumental parameters, were studied. Caffeine and paracetamol detection limits were 0.025 and 0.032 µg/L, and RSD values for 0.6 µg/L caffeine and 0.06 µg/L paracetamol (n = 5) were 1.93 and 2.06%, respectively. Using this technique, some pharmaceuticals containing caffeine and paracetamol were analyzed with satisfactory results.

scholarly journals Spectrodensitometric simultaneous determination of esomeprazole and domperidone in human plasma

2017 ◽  
Vol 15 (1) ◽  
pp. 293-298
Author(s):  
Pakinaz Y. Khashaba ◽  
Hassan Refat H. Ali ◽  
Mohamed M. El-Wekil
Keyword(s):  
Ethyl Acetate ◽  
Factorial Design ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Mobile Phase ◽  
Fractional Factorial Design ◽  
Cost Effective ◽  
Fractional Factorial ◽  
Tlc Plates

AbstractA simple, rapid, cost-effective, and sensitive TLC-spectrodensitometric method for simultaneous determination of esomeprazole and domperidone was developed and tested in human plasma. Ethyl acetate: methanol: benzene: acetonitrile (5: 4: 8: 3, v/v/v/v) mobile phase was used for separation on TLC plates detected at 286 nm. The linearity ranges were 5-1200 and 2-600 ng/ spot for esomeprazole and domperidone, and limits of detection were 1.73 and 0.59 ng/spot. The effects of four variables affecting Rf were evaluated by fractional factorial design. The benzene volume and saturation time had significant effects.

scholarly journals Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 359 ◽  
Author(s):  
Prawez Alam ◽  
Essam Ezzeldin ◽  
Muzaffar Iqbal ◽  
Gamal A.E. Mostafa ◽  
Md. Khalid Anwer ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Solid Lipid Nanoparticles ◽  
Ternary Mixture ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Lipid Nanoparticles ◽  
Ammonia Solution ◽  
Hptlc Method

In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using “RP-18 silica gel 60 F254S HPTLC plates”. However; NP-HPTLC estimation of DLFX was performed using “silica gel 60 F254S HPTLC plates”. For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 v/v/v) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 v/v/v) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at λmax = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX.

Liquid Chromatographic Determination of Simazine, Atrazine, and Propazine Residues in Catfish

1995 ◽  
Vol 78 (4) ◽  
pp. 1067-1071 ◽  
Author(s):  
David C Holland ◽  
Robert K Munns ◽  
José E Roybal ◽  
Jeffrey A Hurlbut ◽  
Austin R Long
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
Triazine Herbicides ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for the simultaneous determination of the triazine herbicides, simazine (SIM), atrazine (ATZ), and propazine (PRO) in the 12.5–100 ppb range in catfish. The herbicides are extracted from catfish homogenates with ethyl acetate, followed by solvent partitioning between acetonitrile and petroleum ether and additional cleanup on a C18 cartridge. A Supelcosil LC-18-DB column is used for LC separation, and UV determination is at 220 nm. The isocratic mobile phase is a mixture of methanol, acetonitrile, and water. Mean recoveries from catfish were 88.7, 96.9, and 91.7%; standard deviations were 6.84,7.78, and 6.26%; and coefficients of variation were 7.72,8.03, and 6.82% for SIM, ATZ, and PRO, respectively.

scholarly journals TLC-spectrodensitometric method for simultaneous determination of dapagliflozin and rosuvastatin in rabbit plasma: stability indicating assay and kinetic studies

RSC Advances ◽  
2020 ◽  
Vol 10 (67) ◽  
pp. 40795-40805
Author(s):  
Noha S. Abbas ◽  
Sayed M. Derayea ◽  
Mahmoud A. Omar ◽  
Gamal A. Saleh
Keyword(s):  
Ethyl Acetate ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
Kinetic Studies ◽  
Urine Samples ◽  
Plasma Stability ◽  
Rabbit Plasma ◽  
Stability Indicating ◽  
Plasma And Urine Samples

Mixtures of DAPA and ROSV were separated using ethyl acetate : methanol (5 : 0.1 v/v) as mobile phase and applied in plasma and urine samples in addition to stability indicating and kinetic studies.

scholarly journals Simultaneous Determination of Diloxanide Furoate and Metronidazole in Presence of Diloxanide Furoate Degradation Products

2011 ◽  
Vol 94 (5) ◽  
pp. 1427-1439
Author(s):  
Samah S Abbas ◽  
Nour E Wagieh ◽  
Mohamed Abdelkawy ◽  
Maha M Abdelrahman
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Orthophosphoric Acid ◽  
Degradation Products ◽  
Potassium Phosphate ◽  
Principal Component Regression ◽  
Principal Component ◽  
Pharmaceutical Formulations ◽  
Dibasic Potassium Phosphate

Abstract Three methods are presented for the simultaneous determination of diloxanide furoate (DLX) and metronidazole (MTR), used for their antiprotozoal and antiamoebic effect, in the presence of DLX alkaline degradates and in pharmaceutical formulations, without previous separation. The first method is chemometric-assisted spectrophotometry, in which principal component regression and partial least squares were applied. These two approaches were successfully applied to quantify each drug in the mixture using the information included in the absorption spectra in the range of 225–320 nm. The second method is TLC-densitometry, in which the binary mixture and degradates were separated on silica gel plates using a chloroform–acetone–glacial acetic acid (9.5 + 0.5 + 0.07, v/v/v) mobile phase and the bands were scanned at 254 nm. The last method is HPLC, in which DLX, MTR, and degradates were separated using the mobile phase acetonitrile–0.05 M dibasic potassium phosphate (25 + 75, v/v), adjusted to pH 4 with orthophosphoric acid, at a flow rate of 1 mL/min, on a C18 analytical column. Detection was at 254 nm. The proposed methods were successfully applied for the analysis of DLX and MTR in pharmaceutical formulations, and the results were statistically compared with a reported spectrophotometric method.

scholarly journals Dual-mode gradient HPLC and TLC densitometry methods for the simultaneous determination of paracetamol and methionine in the presence of paracetamol impurities

2021 ◽  
Author(s):  
Hany Ibrahim ◽  
Abdallah M Hamdy ◽  
Hanan A Merey ◽  
Ahmed S Saad
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Supportive Therapy ◽  
Pharmaceutical Formulations ◽  
Gradient Elution ◽  
Phase System ◽  
Dual Mode ◽  
Chromatographic Methods ◽  
Significant Difference

Abstract Background Paracetamol is one of the most widely analgesic and antipyretic drugs recently integrated into the supportive therapy of COVID-19. The pharmaceuticals containing methionine with paracetamol may contribute to avoid hepatotoxicity and eventual paracetamol overdose-dependent death. Objective The current work purposes to develop and validate two chromatographic methods for the simultaneous determination of methionine and paracetamol in presence of two paracetamol impurities (4-nitrophenol and 4-aminophenol). Methods Two chromatographic methods were established and validated according to the International Conference on Harmonization guidelines. The first one was a RP-HPLC/UV method based on applying a “dual-mode” gradient elution. The separation was realized via varying both the composition of the ternary mobile phase (acetonitrile–methanol–water) and its flow rate. This strategy enabled a relatively rapid analysis with a satisfactory resolution, although the investigated compounds exhibit a significant difference in lipophilicity. The second one relied on TLC- densitometry, where the optimum separation was realized using a quaternary mobile phase system composed of butanol–dioxane–toluene–methanol (8: 2.5: 3.5: 0.3, by volume). Both methods were monitored at 220 nm. Results The developed methods were proven to be robust, accurate, specific, and appropriate for the routine analysis of paracetamol in its pure form or in pharmaceutical formulations with methionine in quality control laboratories. Conclusions The corresponding methods are suitable to determine methionine and paracetamol in the presence of paracetamol impurities. Highlights The study achieves the analysis of methionine and paracetamol in the presence of paracetamol impurities via the application of HPLC and TLC- densitometry methods.

scholarly journals Simultaneous Determination of Acetaminophen, Pamabrom and Pyrilamine Maleate in Pharmaceutical Formulations Using Stability Indicating HPLC Assay Method

2017 ◽  
Vol 59 (2) ◽  
Author(s):  
Muhammad Ashfaq
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Degradation Products ◽  
Pharmaceutical Formulations ◽  
Assay Method ◽  
Good Resolution ◽  
Pharmaceutical Dosage Forms ◽  
Stability Indicating ◽  
Water Ph

A simple, specific and accurate stability indicating RPHPLC method was developed for the determination of acetaminophen, pamabrom and pyrilamine maleate simultaneously in pharmaceutical dosage forms. Successful separation of all the components was enacted within 10 min using C18 column with mobile phase of methanol and acidified water (pH 1.8) in the ratio of (27: 73 v/v respectively). Flow rate of the mobile phase was 1.5 mL/min with detection at 300 nm. The method was validated in accordance with ICH guidelines. Response was a linear function of concentration over the range of 50- 150 􀈝g/mL for acetaminophen, 2.5-7.5 􀈝g/ mL for pamabrom and 1.5-4.5 􀈝g/mL for pyrilamine maleate. The method resulted in excellent separation of all the analytes along with their stress induced degradation products with acceptable peak tailing and good resolution. It is therefore can be applied successfully for simultaneous determination of acetaminophen, pamabrom and pyrilamine maleate in pharmaceutical formulations and their stability studies.

scholarly journals A NOVEL HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF CO-ENZYME Q10 AND α-TOCOPHEROL IN BULK AND PHARMACEUTICAL FORMULATION

2018 ◽  
Vol 10 (10) ◽  
pp. 134 ◽  
Author(s):  
Manasi B. Kulkarni ◽  
Anagha M. Joshi ◽  
Rohini V. Patil
Keyword(s):  
Ethyl Acetate ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
Dosage Form ◽  
Fixed Dose ◽  
Simultaneous Quantification ◽  
Rf Values ◽  
Hptlc Method ◽  
Detection And Quantification

Objective: HPTLC Method for Simultaneous quantification of co-enzyme Q10 and α-tocopherol in bulk and capsule dosage form was developed and validated as per International Conference on Harmonization [(ICH) Q2 (R1)] guideline.Methods: The chromatograms were developed using a mobile phase of Toluene: ethyl acetate: chloroform (10:1:2 v/v/v) on Pre-coated silica 60F 254 plates and quantified by densitometric absorbance mode at 280 nm.Results: The Rf values were 0.77 and 0.87 for co-enzyme Q10 and α-tocopherol, respectively. The linearity of the method was found to be in the concentration range of 0.6µg-1.8 µg/band for α-tocopherol and 2 µg-6 µg/band for co-enzyme Q10. The limits of detection and quantification were 0.3154 and 0.9559 µg/band for α-tocopherol and 3.441 and 10.42 µg/band for co-enzyme Q10.Conclusion: Developed densitometric method was found to be robust, precise, accurate, and rapid and can be used to analyse fixed-dose capsule samples of co-enzyme Q10 and α-tocopherol.

A Green Liquid Chromatographic Method For The Simultaneous Determination of Chlorpheniramine Maleate, Pseudoephedrine Hydrochloride and Propyphenazone

2019 ◽  
Vol 15 (6) ◽  
pp. 635-641
Author(s):  
Nadia M. Mostafa ◽  
Ghada M. Elsayed ◽  
Nagiba Y. Hassan ◽  
Dina A. El Mously
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Dosage Form ◽  
Chromatographic Method ◽  
Green Analytical Chemistry ◽  
Chlorpheniramine Maleate ◽  
Accuracy And Precision ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Background:The concept of green analytical chemistry prevails due to the growing environmental pollution.Objective:Our attempts are to develop simple and eco-friendly method which is non-harmful to the environment by producing minimal waste. In this context, a green liquid chromatographic method was applied for the simultaneous determination of chlorpheniramine maleate, pseudoephedrine hydrochloride and propyphenazone in their combined dosage form.Methods:Separation was carried out using X select HSS RP C18 analytical column (250 × 4.6 mm, 5μm) using methanol - 0.02 M phosphate buffer pH 3 - triethylamine (60:40: 0.1, by volume) as a mobile phase. The separated peaks were detected at 215 nm at a flow rate 1.0 mL/min.Results:Quantification was done over the concentration ranges of 1-25 µg/mL for chlorpheniramine maleate, 5-35 µg/mL for pseudoephedrine hydrochloride and 10-120 µg/mL for propyphenazone. The suggested method was validated with regard to linearity, accuracy and precision according to the International Conference on Harmonization guidelines with good results.Conclusion:It could be used as a safer alternative for routine analysis of the mentioned drugs in quality control laboratories.

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