A single molecule assay for ultrasensitive detection of Fn14 in human serum

2019 ◽  
Vol 587 ◽  
pp. 113467 ◽  
Author(s):  
Yichen Chen ◽  
Qiyong Cai ◽  
Song Liu
Keyword(s):  
Human Serum ◽  
Single Molecule ◽  
Ultrasensitive Detection

A new enzyme-free quadratic SERS signal amplification approach for circulating microRNA detection in human serum

2015 ◽  
Vol 51 (90) ◽  
pp. 16271-16274 ◽  
Author(s):  
Jing Zheng ◽  
Dandan Ma ◽  
Muling Shi ◽  
Junhui Bai ◽  
Yinhui Li ◽  
...  
Keyword(s):  
Human Serum ◽  
Signal Amplification ◽  
Circulating Microrna ◽  
Ultrasensitive Detection ◽  
Circulating Mirna ◽  
Sers Signal ◽  
Microrna Detection

In this communication, we proposed a new enzyme-free quadratic SERS signal amplification approach for the ultrasensitive detection of circulating miRNA in human serum.

Single-molecule FRET for Ultrasensitive Detection of Biomolecules

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Kun Yang ◽  
Yong Yang ◽  
Chun-yang Zhang
Keyword(s):  
Single Molecule ◽  
New Technologies ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Molecular Probes ◽  
Ultrasensitive Detection ◽  
Single Molecule Fret ◽  
Biological Reaction ◽  
Spatio Temporal ◽  
Detection Of Biomolecules

AbstractSingle-molecule Förster resonance energy transfer (sm- FRET) has been widely employed to detect biomarkers and to probe the structure and dynamics of biomolecules. By monitoring the biological reaction in a spatio-temporal manner, smFRET can reveal the transient intermediates of biological processes that cannot be obtained by conventional ensemble measurements. This review provides an overview of singlemolecule FRET and its applications in ultrasensitive detection of biomolecules, including the major techniques and the molecular probes used for smFRET as well as the biomedical applications of smFRET. Especially, the combination of sm- FRET with new technologies might expand its applications in clinical diagnosis and biomedical research

scholarly journals Ultrasensitive Detection of Attomolar Protein Concentrations by Dropcast Single Molecule Assays

2020 ◽  
Vol 142 (28) ◽  
pp. 12314-12323 ◽  
Author(s):  
Connie Wu ◽  
Padric M. Garden ◽  
David R. Walt
Keyword(s):  
Single Molecule ◽  
Ultrasensitive Detection

scholarly journals Ultrasensitive Detection of Enzymatic Activity Using Single Molecule Arrays

2020 ◽  
Vol 142 (35) ◽  
pp. 15098-15106
Author(s):  
Xu Wang ◽  
Alana F. Ogata ◽  
David R. Walt
Keyword(s):  
Enzymatic Activity ◽  
Single Molecule ◽  
Ultrasensitive Detection

Unfolding and refolding of a protein by cholesterol and cyclodextrin: a single molecule study

2015 ◽  
Vol 17 (12) ◽  
pp. 8017-8027 ◽  
Author(s):  
Shirsendu Ghosh ◽  
Catherine Ghosh ◽  
Somen Nandi ◽  
Kankan Bhattacharyya
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Single Molecule ◽  
Globular Protein ◽  
Unfolded Protein

Cholesterol induced unfolding of a globular protein, human serum albumin (HSA), and β-cyclodextrin induced refolding of the unfolded protein is demonstrated in this study.

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