Single-Molecule Arrays for Ultrasensitive Detection of Blood-Based Biomarkers for Immunotherapy

2019 ◽  
pp. 399-412
Author(s):  
Limor Cohen ◽  
Alissa Keegan ◽  
David R. Walt
Keyword(s):  
Single Molecule ◽  
Ultrasensitive Detection

Single-molecule FRET for Ultrasensitive Detection of Biomolecules

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Kun Yang ◽  
Yong Yang ◽  
Chun-yang Zhang
Keyword(s):  
Single Molecule ◽  
New Technologies ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Molecular Probes ◽  
Ultrasensitive Detection ◽  
Single Molecule Fret ◽  
Biological Reaction ◽  
Spatio Temporal ◽  
Detection Of Biomolecules

AbstractSingle-molecule Förster resonance energy transfer (sm- FRET) has been widely employed to detect biomarkers and to probe the structure and dynamics of biomolecules. By monitoring the biological reaction in a spatio-temporal manner, smFRET can reveal the transient intermediates of biological processes that cannot be obtained by conventional ensemble measurements. This review provides an overview of singlemolecule FRET and its applications in ultrasensitive detection of biomolecules, including the major techniques and the molecular probes used for smFRET as well as the biomedical applications of smFRET. Especially, the combination of sm- FRET with new technologies might expand its applications in clinical diagnosis and biomedical research

scholarly journals Ultrasensitive Detection of Attomolar Protein Concentrations by Dropcast Single Molecule Assays

2020 ◽  
Vol 142 (28) ◽  
pp. 12314-12323 ◽  
Author(s):  
Connie Wu ◽  
Padric M. Garden ◽  
David R. Walt
Keyword(s):  
Single Molecule ◽  
Ultrasensitive Detection

scholarly journals Ultrasensitive Detection of Enzymatic Activity Using Single Molecule Arrays

2020 ◽  
Vol 142 (35) ◽  
pp. 15098-15106
Author(s):  
Xu Wang ◽  
Alana F. Ogata ◽  
David R. Walt
Keyword(s):  
Enzymatic Activity ◽  
Single Molecule ◽  
Ultrasensitive Detection

scholarly journals Simultaneous and ultrasensitive detection of multiple microRNAs by single-molecule fluorescence imaging

2020 ◽  
Vol 11 (15) ◽  
pp. 3812-3819 ◽  
Author(s):  
Hongding Zhang ◽  
Xuedong Huang ◽  
Jianwei Liu ◽  
Baohong Liu
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Ultrasensitive Detection ◽  
Single Molecule Fluorescence ◽  
Microrna Detection

A single-molecule assay for multiple microRNA detection.

A single molecule assay for ultrasensitive detection of Fn14 in human serum

2019 ◽  
Vol 587 ◽  
pp. 113467 ◽  
Author(s):  
Yichen Chen ◽  
Qiyong Cai ◽  
Song Liu
Keyword(s):  
Human Serum ◽  
Single Molecule ◽  
Ultrasensitive Detection

scholarly journals Single Molecule Assay for Ultrasensitive Detection of Cathepsin B in Human Blood

ACS Omega ◽  
2021 ◽  
Vol 6 (14) ◽  
pp. 9609-9616
Author(s):  
Bharani Thangavelu ◽  
Angela M. Boutté
Keyword(s):  
Single Molecule ◽  
Human Blood ◽  
Cathepsin B ◽  
Ultrasensitive Detection

A plasmonic aptasensor for ultrasensitive detection of thrombin via arrested rolling circle amplification

2015 ◽  
Vol 51 (37) ◽  
pp. 7927-7930 ◽  
Author(s):  
Sai Wang ◽  
Sai Bi ◽  
Zonghua Wang ◽  
Jianfei Xia ◽  
Feifei Zhang ◽  
...  
Keyword(s):  
Single Molecule ◽  
Rolling Circle Amplification ◽  
Signal Generation ◽  
Ultrasensitive Detection ◽  
Eye Detection ◽  
Rolling Circle ◽  
Single Molecule Level ◽  
Nanoparticle Growth ◽  
Naked Eye ◽  
Naked Eye Detection

A sensitive signal generation mechanism for gold nanoparticle growth is applied in a plasmonic aptasensor, achieving naked-eye detection of thrombin at the single-molecule level.

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