A solid-phase extraction and size-exclusion liquid chromatographic method for polyethylene glycol 25 p-aminobenzoic acid determination in urine: Validation for urinary excretion studies of users of sunscreens

2008 ◽  
Vol 611 (2) ◽  
pp. 220-225 ◽  
Author(s):  
A. Balaguer ◽  
A. Chisvert ◽  
A. Salvador ◽  
M. Herraez ◽  
O. Diez
Keyword(s):  
Polyethylene Glycol ◽  
Urinary Excretion ◽  
Chromatographic Method ◽  
Solid Phase ◽  
Size Exclusion ◽  
Phase Extraction ◽  
Aminobenzoic Acid ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Acid Determination

scholarly journals Determination of Nitroimidazoles and Their Metabolites in Swine Tissues by Liquid Chromatography

2003 ◽  
Vol 86 (3) ◽  
pp. 505-509 ◽  
Author(s):  
Jianzhong Shen ◽  
Yue Yhang ◽  
Suxia Zhang ◽  
Shuangyang Ding ◽  
Xinhua Xiang
Keyword(s):  
Chromatographic Method ◽  
Solid Phase ◽  
Liquid Extraction ◽  
Phase Extraction ◽  
Liquid Liquid Extraction ◽  
Liquid Chromatographic Method ◽  
Swine Tissue ◽  
Liquid Chromatographic ◽  
Sensitive Method

Abstract A liquid chromatographic method was developed for determination of metronidazole (MNZ), ronidazole (RNZ), dimetridazole (DMZ), and 2-hydroxymethyl-1-methyl-5-nitroimidazole (DMZOH) in swine tissue. After extraction with ethyl acetate and evaporation, the nitroimidazoles were redissolved in hydrochloric acid. Hexane was used in the liquid–liquid extraction to remove fat. An Oasis® HLB solid-phase extraction was performed after neutralization of the acidic extract. The limits of detection were 1.0–2.0 μg/kg for DMZOH, MNZ, RNZ, and DMZ in muscle and liver. Average recoveries ranged from 80.1 to 83.9% in muscle fortified at 10, 20, and 50 μg/kg; average re-coveries in liver ranged from 78.9 to 82.3%. The procedure provides a simple and sensitive method for monitoring DMZOH, MNZ, RNZ, and DMZ residues in swine tissues.

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