Rapid synthesis of Au/Ag bimetallic nanoclusters with highly biochemical stability and its applications for temperature and ratiometric pH sensing

2019 ◽  
Vol 1070 ◽  
pp. 88-96 ◽  
Author(s):  
Huanhuan Sun ◽  
Taiping Qing ◽  
Xiaoxiao He ◽  
Jingfang Shangguan ◽  
Ruichen Jia ◽  
...  
Keyword(s):  
Ph Sensing ◽  
Rapid Synthesis ◽  
Bimetallic Nanoclusters ◽  
Ratiometric Ph Sensing ◽  
Biochemical Stability

A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out

2015 ◽  
Vol 51 (28) ◽  
pp. 6145-6148 ◽  
Author(s):  
Robert J. Meier ◽  
Johann M. B. Simbürger ◽  
Tero Soukka ◽  
Michael Schäferling
Keyword(s):  
Dynamic Range ◽  
Dual Wavelength ◽  
Luminescence Lifetime ◽  
Ph Sensing ◽  
Ph Probe ◽  
Broad Dynamic Range ◽  
Europium Chelate ◽  
Ratiometric Ph Sensing

A FRET system composed of a europium chelate and carboxynaphthofluorescein enables ratiometric pH sensing with an exceptionally broad dynamic range.

scholarly journals Synthesis and Antiproliferative Activity of 2,4,6,7-Tetrasubstituted-2H-pyrazolo[4,3-c]pyridines

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6747
Author(s):  
Beatričė Razmienė ◽  
Eva Řezníčková ◽  
Vaida Dambrauskienė ◽  
Radek Ostruszka ◽  
Martin Kubala ◽  
...  
Keyword(s):  
Antiproliferative Activity ◽  
Nuclear Antigen ◽  
Ph Sensing ◽  
Caspase 9 ◽  
Ph Indicator ◽  
Antiproliferative Effects ◽  
Alkylation Reactions ◽  
Ratiometric Ph Sensing ◽  
Proliferating Cell ◽  
Parp 1

A library of 2,4,6,7-tetrasubstituted-2H-pyrazolo[4,3-c]pyridines was prepared from easily accessible 1-phenyl-3-(2-phenylethynyl)-1H-pyrazole-4-carbaldehyde via an iodine-mediated electrophilic cyclization of intermediate 4-(azidomethyl)-1-phenyl-3-(phenylethynyl)-1H-pyrazoles to 7-iodo-2,6-diphenyl-2H-pyrazolo[4,3-c]pyridines followed by Suzuki cross-couplings with various boronic acids and alkylation reactions. The compounds were evaluated for their antiproliferative activity against K562, MV4-11, and MCF-7 cancer cell lines. The most potent compounds displayed low micromolar GI50 values. 4-(2,6-Diphenyl-2H-pyrazolo[4,3-c]pyridin-7-yl)phenol proved to be the most active, induced poly(ADP-ribose) polymerase 1 (PARP-1) cleavage, activated the initiator enzyme of apoptotic cascade caspase 9, induced a fragmentation of microtubule-associated protein 1-light chain 3 (LC3), and reduced the expression levels of proliferating cell nuclear antigen (PCNA). The obtained results suggest a complex action of 4-(2,6-diphenyl-2H-pyrazolo[4,3-c]pyridin-7-yl)phenol that combines antiproliferative effects with the induction of cell death. Moreover, investigations of the fluorescence properties of the final compounds revealed 7-(4-methoxyphenyl)-2,6-diphenyl-2H-pyrazolo[4,3-c]pyridine as the most potent pH indicator that enables both fluorescence intensity-based and ratiometric pH sensing.

scholarly journals Ratiometric pH Sensing and Imaging in Living Cells with Dual-Emission Semiconductor Polymer Dots

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2923
Author(s):  
Piaopiao Chen ◽  
Iqra Ilyas ◽  
Su He ◽  
Yichen Xing ◽  
Zhigang Jin ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescein Isothiocyanate ◽  
Water Soluble ◽  
Ph Sensing ◽  
Dual Emission ◽  
Polymer Dots ◽  
Ratiometric Ph Sensing

Polymer dots (Pdots) represent newly developed semiconductor polymer nanoparticles and exhibit excellent characteristics as fluorescent probes. To improve the sensitivity and biocompatibility of Pdots ratiometric pH biosensors, we synthesized 3 types of water-soluble Pdots: Pdots-PF, Pdots-PP, and Pdots-PPF by different combinations of fluorescent dyes poly(9,9-dioctylfluorenyl-2,7-diyl) (PFO), poly[(9,9-dioctyl-fluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1′,3}-thiadazole)] (PFBT), and fluorescein isothiocyanate (FITC). We found that Pdots-PPF exhibits optimal performance on pH sensing. PFO and FITC in Pdots-PPF produce pH-insensitive (λ = 439 nm) and pH-sensitive (λ = 517 nm) fluorescence respectively upon a single excitation at 380 nm wavelength, which enables Pdots-PPF ratiometric pH sensing ability. Förster resonance energy transfer (FRET) together with the use of PFBT amplify the FITC signal, which enables Pdots-PPF robust sensitivity to pH. The emission intensity ratio (I517/I439) of Pdots-PPF changes linearly as a function of pH within the range of pH 3.0 to 8.0. Pdots-PPF also possesses desirable reversibility and stability in pH measurement. More importantly, Pdots-PPF was successfully used for cell imaging in Hela cells, exhibiting effective cellular uptake and low cytotoxicity. Our study suggests the promising potential of Pdots-PPF as an in vivo biomarker.

Multifunctional PHPMA-Derived Polymer for Ratiometric pH Sensing, Fluorescence Imaging, and Magnetic Resonance Imaging

2018 ◽  
Vol 10 (2) ◽  
pp. 1556-1565 ◽  
Author(s):  
Fengyu Su ◽  
Shubhangi Agarwal ◽  
Tingting Pan ◽  
Yuan Qiao ◽  
Liqiang Zhang ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Fluorescence Imaging ◽  
Ph Sensing ◽  
Resonance Imaging ◽  
Ratiometric Ph Sensing

Synthesis and Characterization of Ratiometric, pH Sensing Nanoparticles with Covalently Attached Fluorescent Dyes

2006 ◽  
Vol 18 (15) ◽  
pp. 3381-3384 ◽  
Author(s):  
Honghao Sun ◽  
Anne Marie Scharff-Poulsen ◽  
Hong Gu ◽  
Kristoffer Almdal
Keyword(s):  
Fluorescent Dyes ◽  
Synthesis And Characterization ◽  
Ph Sensing ◽  
Ratiometric Ph Sensing

Magnetic and fluorescent core–shell nanoparticles for ratiometric pH sensing

2011 ◽  
Vol 22 (41) ◽  
pp. 415501 ◽  
Author(s):  
Alejandro Lapresta-Fernández ◽  
Tristan Doussineau ◽  
Silvio Dutz ◽  
Frank Steiniger ◽  
Artur J Moro ◽  
...  
Keyword(s):  
Core Shell ◽  
Ph Sensing ◽  
Shell Nanoparticles ◽  
Ratiometric Ph Sensing ◽  
Core Shell Nanoparticles

Selective ratiometric pH-sensing PAMAM light-harvesting dendrimer based on Rhodamine 6G and 1,8-naphthalimide

2014 ◽  
Vol 277 ◽  
pp. 62-74 ◽  
Author(s):  
Nikolai I. Georgiev ◽  
Abdullah M. Asiri ◽  
Khalid A. Alamry ◽  
Abdullah Y. Obaid ◽  
Vladimir B. Bojinov
Keyword(s):  
Rhodamine 6G ◽  
Light Harvesting ◽  
Ph Sensing ◽  
Ratiometric Ph Sensing

scholarly journals An Intelligent AIEgen with Nonmonotonic Multi-Responses to Multi-Stimuli

2020 ◽  
Author(s):  
Yujie Tu ◽  
Yeqing Yu ◽  
Diwen Xiao ◽  
Junkai Liu ◽  
zheng zhao ◽  
...  
Keyword(s):  
Rational Design ◽  
Gas Sensing ◽  
Solvent Polarity ◽  
Design Strategy ◽  
Ph Sensing ◽  
Light Emitting ◽  
Stimulus Response ◽  
Donor Acceptor ◽  
Ratiometric Ph Sensing ◽  
Anti Stokes

<p>Intelligent stimulus-response (S/R) systems are the basis of natural process and machine control, and have been intensively explored in biomimetic design, analytical chemistry and biological applications. However, nonmonotonic multi-S/R systems are still rarely studied so far. Now, we propose a rational design strategy to achieve such a unique S/R system by integrating opposite luminescence behaviors in one molecule. When solvent polarity increases, many heterocycles often become more emissive due to the suppression of the proximity effect. However, molecules with donor-acceptor (D-A) structures tend to be less emissive because of the twisted intramolecular charge transfer. Meanwhile, protonation on D/A moieties will weaken/strengthen the D-A interaction to result in blue/red-shifted emissions. By combining a protonatable heterocyclic acceptor and a protonatable donor together in one molecule, we can easily achieve nonmonotonic brightness responses to polarity stimuli and nonmonotonic color responses to pH stimuli. In this work, a simple molecule, namely ASQ is chosen as the model compound to verify the design strategy feasibility. It successfully shows two opposite trends of responses to polarity and pH stimuli, and aggregation-induced emission (AIE) with a nonmonotonic AIE curve. Moreover, the acidified ASQ solution is also a pure organic up-conversion and white-light-emitting system. A new mechanistic viewpoint is established to explain its unique anti-Stokes emission. Besides, ASQ shows multivalent functionalities including albumin protein sensing, ratiometric pH sensing, and amine gas sensing, etc. Therefore, ASQ is proved to be a fundamentally important and versatile functional “intelligent” AIE luminogen with nonmonotonic multi-responses to multi-stimuli. <br></p>

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