Activity-based ratiometric fluorescent small-molecule probe for endogenously monitoring neutrophil elastase in living cells

2020 ◽  
Vol 1127 ◽  
pp. 295-302
Author(s):  
Ting Cao ◽  
Zhidong Teng ◽  
Lei Zheng ◽  
Jing Qian ◽  
Hong Ma ◽  
...  
Keyword(s):  
Small Molecule ◽  
Neutrophil Elastase ◽  
Living Cells ◽  
Small Molecule Probe

scholarly journals Blinking fluorescent probes for tubulin nanoscopy in living and fixed cells

2021 ◽  
Author(s):  
Ruta Gerasimaite ◽  
Jonas Bucevicius ◽  
Kamila A. Kiszka ◽  
Georgij Kostiuk ◽  
Tanja Koenen ◽  
...  
Keyword(s):  
Small Molecule ◽  
Single Molecule ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Stimulated Emission ◽  
Sted Microscopy ◽  
Linker Length ◽  
Localisation Microscopy ◽  
Red Dye ◽  
Small Molecule Probe

Here we report a small molecule probe for single molecule localisation microscopy (SMLM) of tubulin in living and fixed cells. We explored a series of constructs composed of taxanes and spontaneously blinking far-red dye hydroxymethyl silicon-rhodamine (HMSiR). We found that the linker length profoundly affects the probe permeability and off-targeting. The best performing probe, HMSiR-tubulin, is composed of cabazitaxel and 6'-regioisomer of HMSiR bridged by a C6 linker. Microtubule diameters of <50 nm can be routinely measured in SMLM experiments on living and fixed cells. HMSiR-tubulin also performs well in 3D stimulated emission depletion (STED) microscopy, allowing a complementary use of both nanoscopy methods for investigating microtubule functions in living cells.

Small-molecule probe reveals a kinase cascade that links stress signaling to TCF/LEF and Wnt responsiveness

2021 ◽  
Author(s):  
Jiongjia Cheng ◽  
Masanao Tsuda ◽  
Karl Okolotowicz ◽  
Mary Dwyer ◽  
Paul J. Bushway ◽  
...  
Keyword(s):  
Small Molecule ◽  
Stress Signaling ◽  
Kinase Cascade ◽  
Small Molecule Probe

Small-molecule FRET probes for protein kinase activity monitoring in living cells

2010 ◽  
Vol 397 (4) ◽  
pp. 750-755 ◽  
Author(s):  
Angela Vaasa ◽  
Marje Lust ◽  
Anna Terrin ◽  
Asko Uri ◽  
Manuela Zaccolo
Keyword(s):  
Protein Kinase ◽  
Small Molecule ◽  
Kinase Activity ◽  
Living Cells ◽  
Activity Monitoring ◽  
Protein Kinase Activity

Rational design of water-dispersible and biocompatible nanoprobes with H2S-triggered NIR emission for cancer cell imaging

2020 ◽  
Vol 8 (28) ◽  
pp. 6013-6016
Author(s):  
Hengyan Liu ◽  
Ge Xu ◽  
Tianli Zhu ◽  
Rongchen Wang ◽  
Jiahui Tan ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Small Molecule ◽  
Rational Design ◽  
Cell Imaging ◽  
Imaging Modality ◽  
Aqueous Solubility ◽  
Water Dispersible ◽  
Nir Emission ◽  
Small Molecule Probe

A nanoprobe with good aqueous solubility and biocompatibility by trapping an H2S-activatable small molecule probe in the interior of surface cross-linked micelles was fabricated for imaging of H2S-rich cancer cells in a dual-color imaging modality.

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