Enzyme-based amperometric biosensors for malic acid – A review

2021 ◽  
pp. 338218
Author(s):  
Christopher J. Matthews ◽  
Emma S.V. Andrews ◽  
Wayne M. Patrick
2003 ◽  
Vol 8 (1) ◽  
pp. 3-18 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
J. Kulys

A mathematical model of amperometric biosensors has been developed to simulate the biosensor response in stirred as well as non stirred solution. The model involves three regions: the enzyme layer where enzyme reaction as well as mass transport by diffusion takes place, a diffusion limiting region where only the diffusion takes place, and a convective region, where the analyte concentration is maintained constant. Using computer simulation the influence of the thickness of the enzyme layer as well the diffusion one on the biosensor response was investigated. The computer simulation was carried out using the finite difference technique.


2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.


Author(s):  
Bong‐Seop Lee ◽  
Michel Vert ◽  
Eggehard Holler

2011 ◽  
Vol 37 (4) ◽  
pp. 367-371
Author(s):  
Xi-xin ZHOU ◽  
Ji-heng ZHOU ◽  
De-chuan WU ◽  
Zai-dou FAN ◽  
Wen-bi LI ◽  
...  

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 537d-537
Author(s):  
Elise A. Konow ◽  
Yin-Tung Wang

Four-month-old, aseptically raised Phalaenopsis Atien Kaala `TSC 22' seedlings 1.0 cm in leaf spread were transferred (Nov. 1995) 25 per polycarbonate box to an agar medium and placed under 10, 20, 40, or 80 mol·–2·s–1 PPF from cool-white fluorescent tubes. In June 1996, plants grown under 40 or 80 mol·–2·s–1 PPF had greater mass, wider leaves, and more roots than those under the two lower PPF. Plants were then transplanted and grown in a greenhouse (GH) under 340, 170, or 85 mol·–2·s–1maximum PPF. In May 1997, plants previously produced under 40 mol·–2·s–1 PPF had longer, wider, and thicker leaves than those under 10 mol·–2·s–1 PPF when grown under the two higher GH PPF. Under the low GH PPF, however, plants were equally small, regardless of the previous flasking PPF levels. Plants under the high, medium, and low GH PPF had an average of 61, 37, and 17 g of fresh mass, respectively. By September 1997, plants had increasingly larger leaves and higher concentrations of malic acid, sugars, and starch as GH PPF increased. Each doubling in GH PPF resulted in more than a two-fold increase in plant fresh mass. Under the low GH PPF, plants previously produced under 80 mol·–2·s–1 PPF during flasking were 13% larger than those under 10 mol·–2·s–1 PPF. Plants grown under the high, medium, and low GH PPF had 100%, 79%, and 0% flowering, respectively. Those under the high GH PPF bloomed earlier and had longer inflorescences, bearing many more and larger flowers, than those under the medium GH PPF.


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