Selective recognition of a cyclic peptide hormone in human plasma by hydrazone bond-oriented surface imprinted nanoparticles

H-kininogen (high-molecular-mass kininogen, HK) is the precursor of the vasoactive peptide hormone bradykinin (BK). Previous work has demonstrated that HK binds to Streptococcus pyogenesthrough M-proteins, fibrous surface proteins and important virulence factors of these bacteria. Here we find that M-protein-expressing bacteria absorb HK from human plasma. The HK bound to the bacteria was found to be cleaved, and analysis of the degradation pattern suggested that the cleavage of HK at the bacterial surface is associated with the release of BK. Moreover, addition of activated plasma prekallikrein to bacteria preincubated with human plasma, resulted in BK release. This mechanism, by which a potent vasoactive and proinflammatory peptide is generated at the site of infection, should influence the host–parasite relationship during S. pyogenes infections.

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Differential Mobility Spectrometry Coupled with Multiple Ion Monitoring in Regulated LC-MS/MS Bioanalysis of a Therapeutic Cyclic Peptide in Human Plasma

Analytical Chemistry ◽

10.1021/acs.analchem.5b04408 ◽

2016 ◽

Vol 88 (7) ◽

pp. 3655-3661 ◽

Cited By ~ 25

Author(s):

Yunlin Fu ◽

Yuan-Qing Xia ◽

Jimmy Flarakos ◽

Francis L.S. Tse ◽

Jeffrey D. Miller ◽

...

Keyword(s):

Human Plasma ◽

Cyclic Peptide ◽

Differential Mobility Spectrometry ◽

Differential Mobility ◽

Multiple Ion Monitoring

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Neutral cryptand-like cyclic peptide–thiourea receptors for selective recognition of sulphate anions in aqueous solvents

Supramolecular Chemistry ◽

10.1080/10610278.2011.622388 ◽

2011 ◽

Vol 24 (2) ◽

pp. 77-87 ◽

Cited By ~ 11

Author(s):

Philip G. Young ◽

Jack K. Clegg ◽

Katrina A. Jolliffe

Keyword(s):

Cyclic Peptide ◽

Selective Recognition

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Selective recognition of M235T angiotensinogen variants and their determination in human plasma by monoclonal antibody-based immunoanalysis.

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jcem.81.10.8855793 ◽

1996 ◽

Vol 81 (10) ◽

pp. 3505-3512 ◽

Cited By ~ 2

Author(s):

P Cohen ◽

G Badouaille ◽

A P Gimenez-Roqueplo ◽

J C Mani ◽

T T Guyene ◽

...

Keyword(s):

Monoclonal Antibody ◽

Human Plasma ◽

Selective Recognition

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Preprint: A robust peptidomics mass spectrometry platform for measuring oxytocin in plasma and serum

10.1101/042416 ◽

2016 ◽

Cited By ~ 1

Author(s):

Ole Kristian Brandtzaeg ◽

Elin Johnsen ◽

Hanne Roberg-Larsen ◽

Knut Fredrik Seip ◽

Siri Leknes ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Human Plasma ◽

Plasma Proteins ◽

High Performance ◽

Cyclic Peptide ◽

Liquid Chromatography Mass Spectrometry ◽

Cord Serum ◽

Chromatography Mass Spectrometry ◽

Nano Liquid Chromatography

Current approaches to measuring the cyclic peptide oxytocin in plasma/serum are associated with poor selectivity and/or inadequate sensitivity. We here describe a high performance nano liquid chromatography-mass spectrometry platform for measuring OT in human plasma/serum. The platform is extremely robust, allowing laborious sample clean-up steps to be omitted. OT binds strongly to plasma proteins, but a reduction/alkylation procedure breaks this bond, allowing ample detection of total OT. The method showed excellent quantitation properties, and was used to determine total OT levels to 0.5-1.2 ng/mL (evaluated with human plasma and cord serum). The method is compatible with accessible mass spectrometry instrumentation, finally allowing selective and easily comparable oxytocin measurements.

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