Comparative proteomic analysis of surface proteins of Trichinella spiralis muscle larvae and intestinal infective larvae

Acta Tropica ◽  
2015 ◽  
Vol 150 ◽  
pp. 79-86 ◽  
Author(s):  
Ruo Dan Liu ◽  
Jing Cui ◽  
Xiao Lin Liu ◽  
Peng Jiang ◽  
Ge Ge Sun ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Trichinella Spiralis ◽  
Surface Proteins ◽  
Infective Larvae ◽  
Comparative Proteomic Analysis ◽  
Muscle Larvae

scholarly journals Immune response to stage-specific surface antigens of the parasitic nematode Trichinella spiralis.

1981 ◽  
Vol 154 (1) ◽  
pp. 210-215 ◽  
Author(s):  
M Philipp ◽  
P M Taylor ◽  
R M Parkhouse ◽  
B M Ogilvie
Keyword(s):  
Primary Infection ◽  
Time Course ◽  
Parasitic Nematode ◽  
Trichinella Spiralis ◽  
Serum Antibody ◽  
Surface Antigens ◽  
Surface Proteins ◽  
Infective Larvae ◽  
Intestinal Worms

Rats were infected with the nematode Trichinella spiralis and the primary serum antibody response to antigenic surface proteins of infective larvae, intestinal worms, and newborn larvae was studies. 1 wk after infection, the sera contained antibodies to surface antigens of both infective larvae and intestinal worms. These early sera, however, failed to react with newborn larvae surface antigens. In addition, adsorption of sera with living intestinal worms or infective larvae removed antibodies to surface antigens of the homologous stage only. Finally, the time-course of appearance of antibodies that mediate eosinophil adherence to the surface of each stage of the parasite. We concluded that in a primary infection in rats, the surface proteins of T. spiralis used in this study are antigenically stage specific. Furthermore, they could be targets for the stage-specific, antibody-dependent eosinophil-mediated destruction of this parasite, known to occur in vitro.

scholarly journals Label-free quantitative proteomic analysis of molting-related proteins of Trichinella spiralis intestinal infective larvae

2019 ◽  
Vol 50 (1) ◽  
Author(s):  
Hua Nan Ren ◽  
Ruo Dan Liu ◽  
Yan Yan Song ◽  
Tong Xu Zhuo ◽  
Kai Xia Guo ◽  
...  
Keyword(s):  
Hormonal Regulation ◽  
Fatty Acid Biosynthesis ◽  
Trichinella Spiralis ◽  
Arachidonic Acid Metabolism ◽  
New Drugs ◽  
Parasitic Nematodes ◽  
Label Free ◽  
Infective Larvae ◽  
Muscle Larvae ◽  
Related Proteins

Abstract Molting is a key step for body-size expansion and environmental adaptation of parasitic nematodes, and it is extremely important for Trichinella spiralis growth and development, but the molting mechanism is not fully understood. In this work, label-free LC–MS/MS was used to determine the proteome differences between T. spiralis muscle larvae (ML) at the encapsulated stage and intestinal infective larvae (IIL) at the molting stage. The results showed that a total of 2885 T. spiralis proteins were identified, 323 of which were differentially expressed. These proteins were involved in cuticle structural elements, regulation of cuticle synthesis, remodeling and degradation, and hormonal regulation of molting. These differential proteins were also involved in diverse intracellular pathways, such as fatty acid biosynthesis, arachidonic acid metabolism, and mucin type O-glycan biosynthesis. qPCR results showed that five T. spiralis genes (cuticle collagen 14, putative DOMON domain-containing protein, glutamine synthetase, cathepsin F and NADP-dependent isocitrate dehydrogenase) had significantly higher transcriptional levels in 10 h IIL than ML (P < 0.05), which were similar to their protein expression levels, suggesting that they might be T. spiralis molting-related genes. Identification and characterization of T. spiralis molting-related proteins will be helpful for developing vaccines and new drugs against the early enteral stage of T. spiralis.

scholarly journals Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

PLoS ONE ◽  
2018 ◽  
Vol 13 (6) ◽  
pp. e0198205 ◽  
Author(s):  
Xiaoli Wang ◽  
Liang Li ◽  
Xing Wei ◽  
Yuanyuan Wang ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Proteomic Analysis ◽  
Trichinella Spiralis ◽  
Muscle Larvae ◽  
Exogenous Nitric Oxide

scholarly journals Comparative Proteomic Analysis of Serum from Pigs Experimentally Infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis

Pathogens ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 55 ◽  
Author(s):  
Michał Gondek ◽  
Agnieszka Herosimczyk ◽  
Przemysław Knysz ◽  
Małgorzata Ożgo ◽  
Adam Lepczyński ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Trichinella Spiralis ◽  
Uninfected Control ◽  
Complement C3 ◽  
Control Group ◽  
Gamma Chain ◽  
Two Dimensional Gel Electrophoresis ◽  
Trichinella Britovi ◽  
Trichinella Pseudospiralis ◽  
Comparative Proteomic Analysis

Although the available proteomic studies have made it possible to identify and characterize Trichinella stage-specific proteins reacting with infected host-specific antibodies, the vast majority of these studies do not provide any information about changes in the global proteomic serum profile of Trichinella-infested individuals. In view of the above, the present study aimed to examine the protein expression profile of serum obtained at 13 and 60 days postinfection (d.p.i.) from three groups of pigs experimentally infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis and from uninfected, control pigs by two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The comparative proteomic analysis of the T. spiralis group vs. the control group revealed 5 differently expressed spots at both 13 and 60 d.p.i. Experimental infection with T. britovi induced significant expression changes in 3 protein spots at 13 d.p.i. and in 6 protein spots at 60 d.p.i. in comparison with the control group. Paired analyses between the group infected with T. pseudospiralis and the uninfected control group revealed 6 differently changed spots at 13 d.p.i. and 2 differently changed spots at 60 d.p.i. Among these 27 spots, 15 were successfully identified. Depending on the Trichinella species triggering the infection and the time point of serum collection, they were IgM heavy-chain constant region, antithrombin III-precursor, immunoglobulin gamma-chain, clusterin, homeobox protein Mohawk, apolipoprotein E precursor, serum amyloid P-component precursor, Ig lambda chains, complement C3 isoform X1, and apolipoprotein A-I. Our results demonstrate that various Trichinella species and different phases of the invasion produce a distinct, characteristic proteomic pattern in the serum of experimentally infected pigs.

scholarly journals Development of a rapid and sensitive immunochromatographic strip based on EuNPs-ES fluorescent probe for the detection of early Trichinella spiralis-specific IgG antibody in pigs

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Xinyu Wang ◽  
Bin Tang ◽  
Ying Zhao ◽  
Jing Ding ◽  
Nan Wang ◽  
...  
Keyword(s):  
Test Line ◽  
Trichinella Spiralis ◽  
Parasite Infection ◽  
Igg Antibody ◽  
Immunochromatographic Strip ◽  
Muscle Larvae ◽  
Zoonotic Parasite ◽  
Circulating Antibodies ◽  
Novel Method ◽  
Specific Igg

AbstractTrichinellosis, which is caused by nematodes of the genus Trichinella, is one of the most important zoonotic parasite diseases in the world. A rapid and sensitive immunochromatographic strip (ICS) based on Eu (III) nanoparticles (EuNPs) was developed for the detection of Trichinella spiralis (T. spiralis) infection in pigs. T. spiralis muscle larvae excretory secretory or preadult worm excretory secretory (ML-ES or PAW-ES) antigens were conjugated with EuNPs probes to capture T. spiralis-specific antibodies in pig sera, after which the complex bound to mouse anti-pig IgG deposited on the test line (T-line), producing a fluorescent signal. In the pigs infected with 100, 1000 and 10 000 ML, seroconversion was first detectable for the EuNPs-ML-ES ICS at 30, 25 and 21 days post-infection (dpi) and for the EuNPs-PAW-ES ICS at 25, 21 and 17 dpi. These results show that EuNPs-PAW-ES ICS detects anti-Trichinella IgG in pigs 4–5 days earlier that test using ML-ES antigens. Our ICS have no cross reaction with other parasite infection sera. Furthermore, the detection process could be completed in 10 min. This study indicated that our ICS can be used for the detection of the circulating antibodies in early T. spiralis infection and provide a novel method for on-site detection of T. spiralis infection in pigs.

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