scholarly journals Comparative Proteomic Analysis of Serum from Pigs Experimentally Infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis

Pathogens ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 55 ◽  
Author(s):  
Michał Gondek ◽  
Agnieszka Herosimczyk ◽  
Przemysław Knysz ◽  
Małgorzata Ożgo ◽  
Adam Lepczyński ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Trichinella Spiralis ◽  
Uninfected Control ◽  
Complement C3 ◽  
Control Group ◽  
Gamma Chain ◽  
Two Dimensional Gel Electrophoresis ◽  
Trichinella Britovi ◽  
Trichinella Pseudospiralis ◽  
Comparative Proteomic Analysis

Although the available proteomic studies have made it possible to identify and characterize Trichinella stage-specific proteins reacting with infected host-specific antibodies, the vast majority of these studies do not provide any information about changes in the global proteomic serum profile of Trichinella-infested individuals. In view of the above, the present study aimed to examine the protein expression profile of serum obtained at 13 and 60 days postinfection (d.p.i.) from three groups of pigs experimentally infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis and from uninfected, control pigs by two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The comparative proteomic analysis of the T. spiralis group vs. the control group revealed 5 differently expressed spots at both 13 and 60 d.p.i. Experimental infection with T. britovi induced significant expression changes in 3 protein spots at 13 d.p.i. and in 6 protein spots at 60 d.p.i. in comparison with the control group. Paired analyses between the group infected with T. pseudospiralis and the uninfected control group revealed 6 differently changed spots at 13 d.p.i. and 2 differently changed spots at 60 d.p.i. Among these 27 spots, 15 were successfully identified. Depending on the Trichinella species triggering the infection and the time point of serum collection, they were IgM heavy-chain constant region, antithrombin III-precursor, immunoglobulin gamma-chain, clusterin, homeobox protein Mohawk, apolipoprotein E precursor, serum amyloid P-component precursor, Ig lambda chains, complement C3 isoform X1, and apolipoprotein A-I. Our results demonstrate that various Trichinella species and different phases of the invasion produce a distinct, characteristic proteomic pattern in the serum of experimentally infected pigs.

Comparative proteomic analysis on human L-02 liver cells treated with varying concentrations of trichloroethylene

2007 ◽  
Vol 23 (2) ◽  
pp. 91-101 ◽  
Author(s):  
Jianjun Liu ◽  
Haiyan Huang ◽  
Xiumei Xing ◽  
Renrong Xi ◽  
Zhixiong Zhuang ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Underlying Mechanism ◽  
Liver Cells ◽  
Control Group ◽  
Two Dimensional Gel Electrophoresis ◽  
Comparative Proteomic Analysis ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Peptide Mass ◽  
Tof Ms

To determine the differential proteomic expressions in human L-02 liver cells induced by varying concentrations of trichloroethylene (TCE), comparative proteomic analysis was performed on human L-02 liver cells which were treated with varying concentrations of TCE. According to the result of MTT test, we designed four different groups, in which the cells were treated with 0 μM (control group), 3, 10 or 40 μM TCE for 24 h, respectively. Comparative analysis of approximately 800 spots resolved by two-dimensional gel electrophoresis (2DE) in the soluble proteomes of L-02 cells from the four different groups resulted in 10 differential proteins. To identify the differential spots, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) was carried out; if the results from the tool were insufficient, tandem MS (MALDI-TOF-TOF-MS) was then performed. The raw data of peptide mass fingerprints (PMFs) and MS/MS spectra were searched against the IPI human data base for exact matches. Then western blot was employed to verify the result of proteomic analysis, the following result confirmed that the results of proteomic analysis were reliable. These results might provide an insight into the underlying mechanism of TCE intoxication and find biological markers for diagnosis and therapy of TCE-induced diseases.

scholarly journals Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Maria Angeles Gómez-Morales ◽  
Alessandra Ludovisi ◽  
Marco Amati ◽  
Simona Cherchi ◽  
Daniele Tonanzi ◽  
...  
Keyword(s):  
Western Blot ◽  
Trichinella Spiralis ◽  
Trichinella Britovi ◽  
Trichinella Pseudospiralis

scholarly journals Distribution of Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis in the Diaphragms and T. spiralis and T. britovi in the Tongues of Experimentally Infected Pigs

2021 ◽  
Vol 8 ◽  
Author(s):  
Michał Gondek ◽  
Przemysław Knysz ◽  
Renata Pyz-Łukasik ◽  
Anna Łukomska ◽  
Anna Kuriga ◽  
...  
Keyword(s):  
Trichinella Spiralis ◽  
Point Of View ◽  
Homogeneous Distribution ◽  
Infection Level ◽  
Trichinella Britovi ◽  
Trichinella Pseudospiralis ◽  
Epidemiological Surveys ◽  
Inflammatory Pattern ◽  
The Individual ◽  
Pig Carcasses

There is little or even no data in the global literature on the distribution of different species of Trichinella in the individual parts of the diaphragms and tongues in infected pigs. This is of particular importance from the food safety point of view and for the conduct of routine testing of pig carcasses for Trichinella as well as epidemiological surveys. Therefore, the aim of the present study was to evaluate the distribution of Trichinella spiralis (T. spiralis), Trichinella britovi (T. britovi), and Trichinella pseudospiralis (T. pseudospiralis) ML in various parts of the diaphragm (the pillars, costal, and sternal part) and the distribution of encapsulated species of Trichinella (T. spiralis and T. britovi) in various parts of the tongues (the tip, body, and root) of experimentally infected pigs. The diaphragm pillars were the most heavily parasitized part of the diaphragm both in groups of pigs infected with particular species of Trichinella and in groups of pigs presenting different levels of infection; however, statistical differences were observed only in the group of pigs with moderate (21–35 larvae per gram-lpg) or moderately high (35–55 lpg) intensity of Trichinella spp. infection in the entire diaphragm. In all groups of pigs, regardless of the infecting Trichinella species or infection level, larvae showed a homogeneous distribution on both sides of the diaphragm and excluding those of T. pseudospiralis, also in all three parts of the tongue. Histological examination showed features of a differential inflammatory response around larvae of the different Trichinella species. This study confirmed that for mandatory examination of pig carcasses using a pooled-sample digestion assay in which each pig is intended to be represented by a 1 gram sample taken from the diaphragm pillars, if that tissue is not available, the mass of the sample taken from the remaining diaphragm parts (costal or sternal) should be at least double that from the pillars. Histological findings confirmed that the inflammatory pattern of pig muscles varies depending on the Trichinella species triggering the infection and is less intense in the case of infections with T. pseudospiralis than in infections with encapsulated species of Trichinella (T. spiralis and T. britovi).

Comparative Proteomics of Commensal and Pathogenic Strains of Escherichia coli

2020 ◽  
Vol 27 (11) ◽  
pp. 1171-1177
Author(s):  
Neelja Singhal ◽  
Divakar Sharma ◽  
Manish Kumar ◽  
Deepa Bisht ◽  
Jugsharan Singh Virdi
Keyword(s):  
Escherichia Coli ◽  
Proteomic Analysis ◽  
Two Dimensional Gel Electrophoresis ◽  
Maldi Tof Ms ◽  
E Coli ◽  
Comparative Proteomic Analysis ◽  
Maldi Tof ◽  
Northern India ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Background: Most of the proteomic studies in Escherichia coli have focussed on pathogenic strains, while very few studies have studied the commensal strains. It is important to study the commensal strains because under the selective pressure of their habitat, commensal strains might serve as reservoirs of virulent and pathogenic strains. Objective: In this study, we have performed a comparative proteomic analysis of commensal and pathogenic strains of E. coli isolated from a major river flowing through northern India. Methods: Proteins were resolved by two dimensional gel electrophoresis and the differentially expressed proteins were identified using matrix-assisted laser desorption ionization-time of flight mass-spectrometry (MALDI-TOF MS). Results: Many proteins of the commensal strain showed an increased expression compared to the pathogenic strain, of which seventeen proteins were identified by MALDI-TOF MS. Functional classification of these proteins revealed that they belonged to different functional pathways like energy metabolism, nucleotide and nucleoside conversions, translation, biosynthesis of amino acids and motility and energytaxis/chemotaxis. Conclusion: As per the best of our knowledge, this is the first report on comparative proteomic analysis of E. coli commensal and pathogenic strains of aquatic origin. Our results suggest that the increased production of these proteins might play an important role in adaptation of E. coli to a commensal/pathogenic lifestyle. However, further experiments are required to understand the precise role of these proteins in regulating the pathogenicity/commensalism of E. coli.

Reference map and comparative proteomic analysis of Neisseria gonorrhoeae displaying high resistance against spectinomycin

2014 ◽  
Vol 63 (3) ◽  
pp. 371-385 ◽  
Author(s):  
Sunanta Nabu ◽  
Ratana Lawung ◽  
Patcharee Isarankura-Na-Ayudhya ◽  
Chartchalerm Isarankura-Na-Ayudhya ◽  
Sittiruk Roytrakul ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Neisseria Gonorrhoeae ◽  
Protein Expression ◽  
Proteomic Analysis ◽  
Cell Envelope ◽  
Expression Profiles ◽  
Compensatory Mechanisms ◽  
Two Dimensional Gel Electrophoresis ◽  
Comparative Proteomic Analysis ◽  
Reference Map

A proteome reference map of Neisseria gonorrhoeae was successfully established using two-dimensional gel electrophoresis in conjunction with matrix-assisted laser desorption ionization–time of flight mass spectrometry. This map was further applied to compare protein expression profiles of high-level spectinomycin-resistant (clinical isolate) and -susceptible (reference strain) N. gonorrhoeae following treatment with subminimal inhibitory concentrations (subMICs) of spectinomycin. Approximately 200 protein spots were visualized by Coomassie brilliant blue G-250 staining and 66 spots representing 58 unique proteins were subsequently identified. Most of the identified proteins were analysed as cytoplasmic proteins and belonged to the class of energy metabolism. Comparative proteomic analysis of whole protein expression of susceptible and resistant gonococci showed up to 96 % similarity while eight proteins were found to be differentially expressed in the resistant strain. In the presence of subMICs of spectinomycin, it was found that 50S ribosomal protein L7/L12, an essential component for ribosomal translocation, was upregulated in both strains, ranging from 1.5- to 3.5-fold, suggesting compensatory mechanisms of N. gonorrhoeae in response to antibiotic that inhibits protein synthesis. Moreover, the differential expression of proteins involved in energy metabolism, amino acid biosynthesis, and the cell envelope was noticeably detected, indicating significant cellular responses and adaptation against antibiotic stress. Such knowledge provides valuable data, not only fundamental proteomic data, but also knowledge of the mode of action of antibiotic and secondary target proteins implicated in adaptation and compensatory mechanisms.

Abnormal weight gain in mice, induced by Trichinella pseudospiralis

1980 ◽  
Vol 58 (4) ◽  
pp. 614-617 ◽  
Author(s):  
T. O. Al Karmi ◽  
G. M. Faubert
Keyword(s):  
Weight Gain ◽  
Trichinella Spiralis ◽  
Total Body Weight ◽  
Growth Effect ◽  
Control Group ◽  
Trichinella Pseudospiralis ◽  
Muscle Larvae ◽  
Host Parasite Relationship ◽  
Parasite Relationship ◽  
Host Parasite

The parasite-induced weight gain in animals is an unusual phenomenon which involves a complex host–parasite relationship. Trichinella pseudospiralis exhibits such a phenomenon in mice. Eighty Swiss mice were orally infected with 600 T. pseudospiralis or Trichinella spiralis muscle larvae. The total body weight of the mice was recorded at the time of infection and at 2 and 10 months after, and compared with an uninfected control group. Trichinella pseudospiralis muscle larvae induced an abnormal weight gain. This weight increase is statistically different from the other two groups of mice after 2 and 10 months postinfection. The results indicate the existence of a different type of host–parasite relationship between the two parasites. Some speculative ideas are advanced to explain the rapid growth effect of T. pseudospiralis on its host.

scholarly journals Species composition of Trichinella in domestic and wild animals in Bulgaria

2019 ◽  
Vol 22 (1) ◽  
pp. 99-104
Author(s):  
N. Lalkovski
Keyword(s):  
Species Composition ◽  
Molecular Analysis ◽  
Trichinella Spiralis ◽  
Wild Animals ◽  
Widespread Species ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Trichinella Britovi ◽  
Trichinella Pseudospiralis

Four Trichinella species cause trichinellosis in Europe: Trichinella spiralis, Trichinella britovi, Trichinella nativа and Trichinella pseudospiralis. The aim of our study was to determine the preponderance of Trichinella species in Bulgaria. The research covered the period 2010–2016. Molecular analysis was performed with 120 Trichinella isolates. Two species were discovered: Trichinella britovi and Trichinella spiralis. T. britovi predominated over T. spiralis – 113 isolates (94.17%) and 7 (5.83%) respectively. Both species were identified in domestic pigs and wild boars, with T. britovi: T.spiralis ratios in 45:1 in wild boars and 1:1 in domestic pigs. T. britovi was the geographically more widespread species. It was found in samples from domestic and wild animals from all over the country, while T. spiralis has only been found in several areas.

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