Etiology and antimicrobial susceptibility profiles of anaerobic bacteria isolated from clinical samples in a university hospital in Madrid, Spain

Local antimicrobial susceptibility surveys are crucial for optimal empirical therapy guidelines and for aiding in antibiotic stewardship and treatment decisions. For many laboratories, a comprehensive overview of local antimicrobial susceptibility patterns of anaerobic bacteria is still lacking due to the long incubation time and effort involved. The present study investigates the antimicrobial susceptibility patterns and related clinical and demographic data of 2856 clinical isolates of anaerobic bacteria that were submitted for analysis to the Institute for Medical Microbiology and Hygiene of the Freiburg University Medical Center (a tertiary university medical center in Southern Germany) between 2015 and 2019. Antimicrobial susceptibility testing has been carried out according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guideline. Minimum inhibitory concentration (MIC)50 and MIC90 for penicillin, metronidazole, moxifloxacin, and clindamycin were established for Gram-positive anaerobes and for ampicillin-sulbactam, meropenem, metronidazole, moxifloxacin, and clindamycin for Gram-negative anaerobes. The distribution of MIC-values for various antibiotics against anaerobic bacteria was also established, especially for those having no specific breakpoints according to EUCAST guidelines. Most clinically relevant anaerobic bacteria originated from general surgery, neurological, and orthopedic wards. A high proportion of isolates were resistant to moxifloxacin and clindamycin indicating the importance of their susceptibility testing before administration. Based on our study metronidazole and other β-lactam/β-lactamase inhibitor combinations such as ampicillin-sulbactam remain suitable for empirical treatment of infections with anaerobic bacteria.

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Microorganisms involved in Deep Neck Infection (DNIs) in Greece: detection, identification and susceptibility to antimicrobials

10.21203/rs.2.10100/v1 ◽

2019 ◽

Author(s):

Despoina Beka ◽

Vasileios A. Lachanas ◽

Stergios Doumas ◽

Stelios Xytsas ◽

Anastasios Kanatas ◽

...

Keyword(s):

16S Rrna ◽

Anaerobic Bacteria ◽

Neck Surgery ◽

Fusobacterium Necrophorum ◽

Epidemiological Studies ◽

University Hospital ◽

Clinical Samples ◽

Deep Neck Infection ◽

Female Ratio ◽

Neck Infection

Abstract Background To determine, from October 2010 to October 2018, the epidemiology of Deep Neck Infections (DNIs), regarding the detection, the identification and the susceptibility to antimicrobials of causative microorganisms, in Thessaly-Central Greece. Methods An analysis of data from a prospective database was conducted on 610 consecutive patients with DNIs treated in the Otolaryngology / Head & Neck Surgery Department of University Hospital of Larissa. Demographics, clinical features and microbiological data were analyzed. Results Among the 610 patients (1,9/1 male to female ratio, mean age: 39,24±17,25) with DNIs, 584 had a single space (95,7%), while the remaining had a multi-space (4,3%) DNI. The most common areas affected were the peritonsillar space (84,6%) followed by the submandibular space (6,5%). Clinical samples were obtained in 462 patients, and were tested by culture and by the application of 16S rRNA PCR. One hundred fifty-five samples (55,2%) gave positive cultures, in which Streptococcus pyogenes and Staphylococcus aureus were predominant. The application 16S rRNA PCR revealed that 183 samples (39,6%) were positive for bacterial DNA; 22 of them were culture negative and were positive for anaerobic (Fusobacterium necrophorum, Actinomyces israellii etc) and for fastidious microorganisms (Brucella mellitensis, Mycobacterium avium). Conclusion DNIs represent a medical and surgical emergency and evidence-guided empirical treatment with intravenous infusion of antibiotics at the time of diagnosis is mandatory, highlighting the importance of epidemiological studies regarding the causative microorganisms. Although, in our study, the predominant pathogens were S. pyogenes and S. aureus, the combination of culture and molecular assay revealed that anaerobic bacteria play also a significant role in the pathogenesis of DNIs.

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Microorganisms involved in Deep Neck Infection (DNIs) in Greece: detection, identification and susceptibility to antimicrobials

10.21203/rs.2.10100/v3 ◽

2019 ◽

Author(s):

Despoina Beka ◽

Vasileios A. Lachanas ◽

Stergios Doumas ◽

Stelios Xytsas ◽

Anastasios Kanatas ◽

...

Keyword(s):

16S Rrna ◽

Anaerobic Bacteria ◽

Neck Surgery ◽

Fusobacterium Necrophorum ◽

Epidemiological Studies ◽

University Hospital ◽

Clinical Samples ◽

Deep Neck Infection ◽

Female Ratio ◽

Neck Infection

Abstract ABSTRACT Background To determine, from October 2010 to October 2018, the epidemiology of Deep Neck Infections (DNIs), regarding the detection, the identification and the susceptibility to antimicrobials of causative microorganisms, in Thessaly-Central Greece. Methods An analysis of data from a prospective database was conducted on 610 consecutive patients with DNIs treated in the Otolaryngology / Head & Neck Surgery Department of University Hospital of Larissa. Demographics, clinical features and microbiological data were analyzed. Results Among the 610 patients (1,9/1 male to female ratio, mean age: 39,24±17,25) with DNIs, 584 had a single space (95,7%), while the remaining had a multi-space (4,3%) DNI. The most common areas affected were the peritonsillar space (84,6%) followed by the submandibular space (6,5%). Clinical samples were obtained from 462 patients, and were tested by culture and by the application of 16S rRNA PCR. Two hundred fifty-five samples (55,2%) gave positive cultures, in which Streptococcus pyogenes and Staphylococcus aureus were predominant. The application of the 16S rRNA PCR revealed that 183 samples (39,6%) were positive for bacterial DNA; 22 of them, culture negative, were found to be positive for anaerobic ( Fusobacterium necrophorum, Actinomyces israellii etc) and for fastidious microorganisms ( Brucella mellitensis, Mycobacterium avium ). Conclusion DNIs represent a medical and surgical emergency and evidence-guided empirical treatment with intravenous infusion of antibiotics at the time of diagnosis is mandatory, highlighting the importance of epidemiological studies regarding the causative microorganisms. Although, in our study, the predominant pathogens were S. pyogenes and S. aureus, the combination of culture and molecular assay revealed that anaerobic bacteria play also a significant role in the pathogenesis of DNIs.

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Antimicrobial susceptibility profiles of anaerobic bacteria, isolated from human clinical specimens, within different European and surrounding countries. A joint ESGAI study

Anaerobe ◽

10.1016/j.anaerobe.2019.102111 ◽

2020 ◽

Vol 61 ◽

pp. 102111 ◽

Cited By ~ 5

Author(s):

A.C.M. Veloo ◽

H. Bahar Tokman ◽

H. Jean-Pierre ◽

Y. Dumont ◽

S. Jeverica ◽

...

Keyword(s):

Antimicrobial Susceptibility ◽

Anaerobic Bacteria ◽

Clinical Specimens ◽

Susceptibility Profiles

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Nosocomial and community acquired uropathogenic isolates of Proteus mirabilis and antimicrobial susceptibility profiles at a university hospital in Sub–Saharan Africa

Asian Pacific Journal of Tropical Disease ◽

10.1016/s2222-1808(12)60003-6 ◽

2012 ◽

Vol 2 (1) ◽

pp. 7-11 ◽

Cited By ~ 5

Author(s):

GTA Jombo ◽

UE Emanghe ◽

EN Amefule ◽

JG Damen

Keyword(s):

Antimicrobial Susceptibility ◽

Proteus Mirabilis ◽

Sub Saharan Africa ◽

University Hospital ◽

Sub Saharan ◽

Susceptibility Profiles

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Epidemiology ofAcinetobacter baumanniiin a University Hospital in Turkey

Infection Control and Hospital Epidemiology ◽

10.1086/503349 ◽

2006 ◽

Vol 27 (4) ◽

pp. 404-408 ◽

Cited By ~ 11

Author(s):

Halis Akalin ◽

Cüneyt Özakin ◽

Suna Gedikoğlu

Keyword(s):

Acinetobacter Baumannii ◽

Dna Analysis ◽

University Hospital ◽

Clinical Samples ◽

Epidemiological Analysis ◽

The Third ◽

Epidemiologic Surveillance ◽

Genotype C ◽

Susceptibility Profiles ◽

Genotype A

Objective.Molecular epidemiologic surveillance ofAcinetobacter baumanniiby polymerase chain reaction-randomly amplified polymorphic DNA analysis in a university hospital for 3 consecutive study periods.Results.Twelve differentAcinetobacter baumanniigenotypes (A-L) were detected. Although only 2 genotypes were detected during the first period and genotype A appeared to be the most common genotype, genotype D was included in these genotypes during the second study period. Genotype A completely disappeared during the third period. Although the presence of genotype C and the genotype D continued during the third period, 9 new genotypes were detected during this period. Genotype A appeared to be the most common genotype in the hospital (detected in 19 different clinics). The distribution of genotypes in clinical samples correlated with patient traffic between them. Some genotypes were found in both clinical and environmental samples. Seventeen different antibiotypes were detected, according to antibiotic susceptibility profiles.Conclusions.Environmental contamination, airborne transmission, patient transfer, and cross-contamination play important roles in epidemics caused byA. baumanniiin our hospital. The distribution of genotypes can change over time, so antibiotyping is not appropriate for the epidemiological analysis ofA. baumaniiinfection.

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Microorganisms involved in Deep Neck Infection (DNIs) in Greece: detection, identification and susceptibility to antimicrobials

10.21203/rs.2.10100/v4 ◽

2019 ◽

Author(s):

Despoina Beka ◽

Vasileios A. Lachanas ◽

Stergios Doumas ◽

Stelios Xytsas ◽

Anastasios Kanatas ◽

...

Keyword(s):

16S Rrna ◽

Anaerobic Bacteria ◽

Neck Surgery ◽

Fusobacterium Necrophorum ◽

Epidemiological Studies ◽

University Hospital ◽

Clinical Samples ◽

Deep Neck Infection ◽

Female Ratio ◽

Neck Infection

Abstract ABSTRACT Background To determine, from October 2010 to October 2018, the epidemiology of Deep Neck Infections (DNIs), regarding the detection, the identification and the susceptibility to antimicrobials of causative microorganisms, in Thessaly-Central Greece. Methods An analysis of data from a prospective database was conducted on 610 consecutive patients with DNIs treated in the Otolaryngology / Head & Neck Surgery Department of University Hospital of Larissa. Demographics, clinical features and microbiological data were analyzed. Results Among the 610 patients (1,9/1 male to female ratio, mean age: 39,24±17,25) with DNIs, 584 had a single space (95,7%), while the remaining had a multi-space (4,3%) DNI. The most common areas affected were the peritonsillar space (84,6%) followed by the submandibular space (6,5%). Clinical samples were obtained from 462 patients, and were tested by culture and by the application of 16S rRNA PCR. Two hundred fifty-five samples (55,2%) gave positive cultures, in which Streptococcus pyogenes and Staphylococcus aureus were predominant. The application of the 16S rRNA PCR revealed that 183 samples (39,6%) were positive for bacterial DNA; 22 of them, culture negative, were found to be positive for anaerobic ( Fusobacterium necrophorum, Actinomyces israellii etc) and for fastidious microorganisms ( Brucella mellitensis, Mycobacterium avium ). Conclusion DNIs represent a medical and surgical emergency and evidence-guided empirical treatment with intravenous infusion of antibiotics at the time of diagnosis is mandatory, highlighting the importance of epidemiological studies regarding the causative microorganisms. Although, in our study, the predominant pathogens were S. pyogenes and S. aureus, the combination of culture and molecular assay revealed that anaerobic bacteria play also a significant role in the pathogenesis of DNIs.

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