Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender

2016 ◽  
Vol 164 ◽  
pp. 97-104 ◽  
Author(s):  
Burcu Ustuner ◽  
Selim Alcay ◽  
M. Berk Toker ◽  
Zekariya Nur ◽  
Elif Gokce ◽  
...  
Aquaculture ◽  
2020 ◽  
Vol 529 ◽  
pp. 735653
Author(s):  
Marco Birolo ◽  
Francesco Bordignon ◽  
Angela Trocino ◽  
Luca Fasolato ◽  
Antón Pascual ◽  
...  

2006 ◽  
Vol 49 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Alexandre Ninhaus-Silveira ◽  
Fausto Foresti ◽  
Yara Aiko Tabata ◽  
Marcos Guilherme Rigolino ◽  
Rosicleire Veríssimo-Silveira

Cryopreservation of semen from sex-reversed females of rainbow trout aims at rationalizing the production of stocks composed by 100% females. Semen from normal males (M) and two types of genotypic females (R and G), sex-reversed by the oral administration of 17alpha-methyltestosterone, were used. R was obtained by the fertilization of normal eggs with semen of sex-reversed females while G via gynogenetic reproduction. Semen was diluted in an extender solution (glucose 5,4 g, egg yolk 10 ml, dimetil sulfoxide 10 ml, water 80 ml) at 1:3 ratio (semen/extender), stored in straws of 0.5 ml and freezed in a dry container Cryopac CP-65, at -180ºC. Thawing was performed with water at 70ºC for 3 seconds. There were no significant fertilization rate differences (P>0.05) among thawed semen groups (M = 73.1±11.5%; R = 67.2±23.6%; G = 64±5.8%), confirming that the freezing methodology used was efficient to cryopreserve semen of all three trout groups.


Aquaculture ◽  
2004 ◽  
Vol 231 (1-4) ◽  
pp. 23-36 ◽  
Author(s):  
Nikoo Arasteh ◽  
A.-H. Aminirissehei ◽  
A.N. Yousif ◽  
L.J. Albright ◽  
T.D. Durance

1995 ◽  
Vol 52 (5) ◽  
pp. 892-896 ◽  
Author(s):  
Kazuo Araki ◽  
Hisako Shinma ◽  
Hiroyuki Nagoya ◽  
Ichiro Nakayama ◽  
Hiroshi Onozato

Androgenesis is a technique that produces individuals with all of their chromosomes from male parents. It can be useful in generating homozygous lines of fish, and in the recovery of species from cryopreserved sperm. We produced androgenetic diploids by using fused sperm to fertilize eggs. Eggs of albino "Yellow" rainbow trout (Oncorhynchus mykiss) were exposed to 60Co γ-ray irradiation to inactivate the maternally-derived nuclear DNA, and were then fertilized with wild-type rainbow trout sperm fused using an artificial seminal plasma containing 85 mM CaCl2. Embryos that reached the hatching stage (average 0.11%) were pigmented. The nuclear DNA content of cells from newly hatched fry was the same as the DNA content of erythrocyte nuclei of normal diploids.


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