Androgenetic diploids of rainbow trout (Oncorhynchus mykiss) produced by fused sperm

1995 ◽  
Vol 52 (5) ◽  
pp. 892-896 ◽  
Author(s):  
Kazuo Araki ◽  
Hisako Shinma ◽  
Hiroyuki Nagoya ◽  
Ichiro Nakayama ◽  
Hiroshi Onozato
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Seminal Plasma ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Wild Type ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Cryopreserved Sperm ◽  
Γ Ray

Androgenesis is a technique that produces individuals with all of their chromosomes from male parents. It can be useful in generating homozygous lines of fish, and in the recovery of species from cryopreserved sperm. We produced androgenetic diploids by using fused sperm to fertilize eggs. Eggs of albino "Yellow" rainbow trout (Oncorhynchus mykiss) were exposed to 60Co γ-ray irradiation to inactivate the maternally-derived nuclear DNA, and were then fertilized with wild-type rainbow trout sperm fused using an artificial seminal plasma containing 85 mM CaCl2. Embryos that reached the hatching stage (average 0.11%) were pigmented. The nuclear DNA content of cells from newly hatched fry was the same as the DNA content of erythrocyte nuclei of normal diploids.

Identification of chromosomal deficiency by flow cytometry and cytogenetics in mutant tomato (Solanum lycopersicum, Solanaceae) plants

2009 ◽  
Vol 57 (5) ◽  
pp. 444 ◽  
Author(s):  
Isane Vera Karsburg ◽  
Carlos Roberto Carvalho ◽  
Wellington Ronildo Clarindo
Keyword(s):  
Flow Cytometry ◽  
Solanum Lycopersicum ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Chromosome 1 ◽  
Wild Type ◽  
Extrinsic Factors ◽  
Solanum Lycopersicum L ◽  
Morphogenic Process

Structural chromosomal aberrations can occur spontaneously in plant karyotypes as a result of both intrinsic and extrinsic factors. These aberrations may affect sporophyte fitness because fundamental genes involved with distinct morphogenic process may be lost. Inadequate development of flowers and anomalous fruits without seeds has been observed in plants of Solanum lycopersicum L. (Solanaceae) ‘BHG 160’ of the tomato germplasm bank (Universidade Federal de Viçosa, Brazil). The nuclear DNA content, quantified by flow cytometry, showed that mutant ‘BHG 160’ possesses 0.09 pg (4.59%) less nuclear DNA content than does the wild-type ‘BGH 160’. Improved cytogenetical preparations evidenced that this difference was due to a spontaneous terminal deficiency in the short arm of the mutant ‘BGH 160’ Chromosome 1. These results suggest that the genes encoded in the short arm of Chromosome 1 may be involved in the development of flowers and fruits in the tomato.

Maturation of spermatozoa from rainbow trout (Oncorhynchus mykiss) sex-reversed females using artificial seminal plasma or glucose–methanol extender

2015 ◽  
Vol 83 (7) ◽  
pp. 1213-1218 ◽  
Author(s):  
Andrzej Ciereszko ◽  
Grzegorz J. Dietrich ◽  
Joanna Nynca ◽  
Stefan Dobosz ◽  
Janusz Krom
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Seminal Plasma ◽  
Rainbow Trout Oncorhynchus Mykiss

Protective role of ascorbic acid against damage to male germ cells in rainbow trout (Oncorhynchus mykiss)

1999 ◽  
Vol 56 (2) ◽  
pp. 178-183 ◽  
Author(s):  
Andrzej Ciereszko ◽  
Konrad Dabrowski ◽  
Feng Lin ◽  
Li Liu
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Vitamin C ◽  
Oncorhynchus Mykiss ◽  
Seminal Plasma ◽  
Germ Cells ◽  
Plasma Ascorbic Acid ◽  
Male Germ Cells ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Low Levels

Low antioxidant levels have been implicated in damage to sperm DNA. We used a teleost fish to test if low paternal vitamin C status may cause mutations to the sperm and birth defects. During spermatogenesis, rainbow trout (Oncorhynchus mykiss) males were fed diets with graded levels of ascorbic acid derivative that were below and severalfold higher than those required for maximum growth. This treatment resulted in corresponding changes in levels of vitamin C in seminal plasma. We found that low levels of vitamin C in seminal plasma of rainbow trout were associated with a high percentage of abnormal embryos in the offspring. Among abnormal embryos, 34.8% were haploids or aneuploids, as revealed by flow cytometric measurement of DNA content. We found that UV-irradiated sperm gave rise to progeny with abnormalities similar to those resulting from sperm with low levels of antioxidants in seminal plasma. Ascorbic acid applied directly to semen did not prevent damage due to UV irradiation. These results provide the first experimental evidence that the lack or low levels of ascorbic acid in semen correlate with damage to male germ cells.

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