Effects of antifreeze proteins on cryopreserved sterlet (Acipenser ruthenus) sperm motility variables and fertilization capacity

2018 ◽  
Vol 196 ◽  
pp. 143-149 ◽  
Author(s):  
Miaomiao Xin ◽  
Vladimíra Tučková ◽  
Marek Rodina ◽  
Vitaliy Kholodnyy ◽  
Hadiseh Dadras ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Antifreeze Proteins ◽  
Acipenser Ruthenus ◽  
Fertilization Capacity ◽  
Sterlet Acipenser Ruthenus

Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson
Keyword(s):  
Sperm Motility ◽  
Seminal Fluid ◽  
Mature Male ◽  
Sperm Maturation ◽  
Wolffian Duct ◽  
Testicular Spermatozoa ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus ◽  
Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

Effects of antifreeze proteins on cryopreserved sterlet, Acipenser ruthenus sperm quality

Cryobiology ◽  
2018 ◽  
Vol 85 ◽  
pp. 184
Author(s):  
Miaomiao Xin ◽  
Jan Sterba ◽  
Anna Shaliutina-Kolesova ◽  
Borys Dzyuba ◽  
Serhii Boryshpolets ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Antifreeze Proteins ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus

Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

2018 ◽  
Vol 44 (6) ◽  
pp. 1527-1533 ◽  
Author(s):  
Miaomiao Xin ◽  
Jan Sterba ◽  
Anna Shaliutina-Kolesova ◽  
Borys Dzyuba ◽  
Jaroslava Lieskovska ◽  
...  
Keyword(s):  
Antifreeze Proteins ◽  
Protective Role ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus

Improving motility and fertilization capacity of low-quality sperm of sterlet Acipenser ruthenus during storage

2020 ◽  
Vol 156 ◽  
pp. 90-96 ◽  
Author(s):  
Miaomiao Xin ◽  
Yu Cheng ◽  
Marek Rodina ◽  
Vladimíra Tučková ◽  
William L. Shelton ◽  
...  
Keyword(s):  
Acipenser Ruthenus ◽  
Fertilization Capacity ◽  
Sterlet Acipenser Ruthenus

Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome

2018 ◽  
Vol 192 ◽  
pp. 280-289 ◽  
Author(s):  
Miaomiao Xin ◽  
Anna Shaliutina-Kolesova ◽  
Jan Sterba ◽  
Peter Konik ◽  
Sergii Boryshpolets ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus

scholarly journals Ancient Sturgeons Possess Effective DNA Repair Mechanisms: Influence of Model Genotoxicants on Embryo Development of Sterlet, Acipenser ruthenus

2020 ◽  
Vol 22 (1) ◽  
pp. 6
Author(s):  
Ievgeniia Gazo ◽  
Roman Franěk ◽  
Radek Šindelka ◽  
Ievgen Lebeda ◽  
Sahana Shivaramu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Damage ◽  
Dna Repair ◽  
Embryo Development ◽  
Hatching Rate ◽  
Embryo Survival ◽  
Dna Repair Mechanisms ◽  
Acipenser Ruthenus ◽  
Repair Mechanisms ◽  
Sterlet Acipenser Ruthenus

DNA damage caused by exogenous or endogenous factors is a common challenge for developing fish embryos. DNA damage repair (DDR) pathways help organisms minimize adverse effects of DNA alterations. In terms of DNA repair mechanisms, sturgeons represent a particularly interesting model due to their exceptional genome plasticity. Sterlet (Acipenser ruthenus) is a relatively small species of sturgeon. The goal of this study was to assess the sensitivity of sterlet embryos to model genotoxicants (camptothecin, etoposide, and benzo[a]pyrene), and to assess DDR responses. We assessed the effects of genotoxicants on embryo survival, hatching rate, DNA fragmentation, gene expression, and phosphorylation of H2AX and ATM kinase. Exposure of sterlet embryos to 1 µM benzo[a]pyrene induced low levels of DNA damage accompanied by ATM phosphorylation and xpc gene expression. Conversely, 20 µM etoposide exposure induced DNA damage without activation of known DDR pathways. Effects of 10 nM camptothecin on embryo development were stage-specific, with early stages, before gastrulation, being most sensitive. Overall, this study provides foundational information for future investigation of sterlet DDR pathways.

Ultrastructural Localization of Intracellular Calcium During Spermatogenesis of Sterlet (Acipenser ruthenus)

2016 ◽  
Vol 22 (6) ◽  
pp. 1155-1161 ◽  
Author(s):  
Amin Golpour ◽  
Martin Pšenička ◽  
Hamid Niksirat
Keyword(s):  
Intracellular Calcium ◽  
Developmental Stages ◽  
Physiological Function ◽  
Male Gamete ◽  
Ultrastructural Localization ◽  
Acrosomal Vesicle ◽  
Acipenser Ruthenus ◽  
Calcium Deposits ◽  
Late Stages ◽  
Sterlet Acipenser Ruthenus

AbstractCalcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. The aim of this study was to localize and quantify the intracellular distribution of calcium during different developmental stages of spermatogenesis in sterlet, Acipenser ruthenus, using a combined oxalate–pyroantimonate technique. The distribution of calcium was described in spermatogonium, spermatocyte, spermatid, and spermatozoon stages. In the spermatogonium and spermatocyte, calcium deposits were mainly localized in the nucleus and cytoplasm. The spermatid had calcium in the nucleus, developing acrosomal vesicle, and cytoplasm. Intracellular calcium transformed from scattered deposits in spermatogonia and spermatocyte stages into an unbound form in spermatid and the spermatozoon. The proportion of area covered by calcium increased significantly (p<0.05) from early to late stages of spermatogenesis. The largest proportion of area covered by calcium was observed in the nucleus of the spermatozoon. In conclusion, although most of the intracellular calcium is deposited in limited areas of the spermatogonium and spermatocyte, it is present an unbound form in the larger area of spermatids and spermatozoa which probably reflects changes in its physiological function and homeostasis during the process of male gamete production in spermatogenesis.

The role of the IGF-I system for vitellogenesis in maturing female sterlet, Acipenser ruthenus Linnaeus, 1758

2007 ◽  
Vol 150 (1) ◽  
pp. 140-150 ◽  
Author(s):  
S. Wuertz ◽  
A. Nitsche ◽  
M. Jastroch ◽  
J. Gessner ◽  
M. Klingenspor ◽  
...  
Keyword(s):  
Acipenser Ruthenus ◽  
Igf I ◽  
Sterlet Acipenser Ruthenus
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