Effects of antifreeze proteins on cryopreserved sterlet, Acipenser ruthenus sperm quality

DNA damage caused by exogenous or endogenous factors is a common challenge for developing fish embryos. DNA damage repair (DDR) pathways help organisms minimize adverse effects of DNA alterations. In terms of DNA repair mechanisms, sturgeons represent a particularly interesting model due to their exceptional genome plasticity. Sterlet (Acipenser ruthenus) is a relatively small species of sturgeon. The goal of this study was to assess the sensitivity of sterlet embryos to model genotoxicants (camptothecin, etoposide, and benzo[a]pyrene), and to assess DDR responses. We assessed the effects of genotoxicants on embryo survival, hatching rate, DNA fragmentation, gene expression, and phosphorylation of H2AX and ATM kinase. Exposure of sterlet embryos to 1 µM benzo[a]pyrene induced low levels of DNA damage accompanied by ATM phosphorylation and xpc gene expression. Conversely, 20 µM etoposide exposure induced DNA damage without activation of known DDR pathways. Effects of 10 nM camptothecin on embryo development were stage-specific, with early stages, before gastrulation, being most sensitive. Overall, this study provides foundational information for future investigation of sterlet DDR pathways.

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Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

Reproduction Fertility and Development ◽

10.1071/rd16145 ◽

2017 ◽

Vol 29 (7) ◽

pp. 1319 ◽

Cited By ~ 4

Author(s):

Olga Bondarenko ◽

Borys Dzyuba ◽

Marek Rodina ◽

Jacky Cosson

Keyword(s):

Sperm Motility ◽

Seminal Fluid ◽

Mature Male ◽

Sperm Maturation ◽

Wolffian Duct ◽

Testicular Spermatozoa ◽

Acipenser Ruthenus ◽

Sterlet Acipenser Ruthenus ◽

Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

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Ultrastructural Localization of Intracellular Calcium During Spermatogenesis of Sterlet (Acipenser ruthenus)

Microscopy and Microanalysis ◽

10.1017/s1431927616011958 ◽

2016 ◽

Vol 22 (6) ◽

pp. 1155-1161 ◽

Cited By ~ 13

Author(s):

Amin Golpour ◽

Martin Pšenička ◽

Hamid Niksirat

Keyword(s):

Intracellular Calcium ◽

Developmental Stages ◽

Physiological Function ◽

Male Gamete ◽

Ultrastructural Localization ◽

Acrosomal Vesicle ◽

Acipenser Ruthenus ◽

Calcium Deposits ◽

Late Stages ◽

Sterlet Acipenser Ruthenus

AbstractCalcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. The aim of this study was to localize and quantify the intracellular distribution of calcium during different developmental stages of spermatogenesis in sterlet, Acipenser ruthenus, using a combined oxalate–pyroantimonate technique. The distribution of calcium was described in spermatogonium, spermatocyte, spermatid, and spermatozoon stages. In the spermatogonium and spermatocyte, calcium deposits were mainly localized in the nucleus and cytoplasm. The spermatid had calcium in the nucleus, developing acrosomal vesicle, and cytoplasm. Intracellular calcium transformed from scattered deposits in spermatogonia and spermatocyte stages into an unbound form in spermatid and the spermatozoon. The proportion of area covered by calcium increased significantly (p<0.05) from early to late stages of spermatogenesis. The largest proportion of area covered by calcium was observed in the nucleus of the spermatozoon. In conclusion, although most of the intracellular calcium is deposited in limited areas of the spermatogonium and spermatocyte, it is present an unbound form in the larger area of spermatids and spermatozoa which probably reflects changes in its physiological function and homeostasis during the process of male gamete production in spermatogenesis.

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The role of the IGF-I system for vitellogenesis in maturing female sterlet, Acipenser ruthenus Linnaeus, 1758

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2006.07.005 ◽

2007 ◽

Vol 150 (1) ◽

pp. 140-150 ◽

Cited By ~ 36

Author(s):

S. Wuertz ◽

A. Nitsche ◽

M. Jastroch ◽

J. Gessner ◽

M. Klingenspor ◽

...

Keyword(s):

Acipenser Ruthenus ◽

Igf I ◽

Sterlet Acipenser Ruthenus

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Impact of cryopreservation on lipid composition of reproductive cells of male sterlets (Acipenser Ruthenus L.)

Agricultural science and practice ◽

10.15407/agrisp5.01.073 ◽

2018 ◽

Vol 5 (1) ◽

pp. 73-83

Author(s):

L. Drahan ◽

S. Veselsky ◽

Yu. Rud ◽

L. Buchatsky

Keyword(s):

Lipid Composition ◽

Low Temperatures ◽

Neutral Lipids ◽

Sperm Quality ◽

Phospholipid Bilayer ◽

Standard Equipment ◽

Slowing Down ◽

Acipenser Ruthenus ◽

Adaptive Property ◽

The Impact

Aim. To estimate the impact of low temperatures on the lipid composition of reproductive cells of male sterlets (Acipenser ruthenus L) in cryopreservation environment. Methods. The determination of sperm quality (color, consistence, concentration and motility of spermatozoa) was estimated by common biochemical methods using standard equipment. Thin-layer chromatography was used to reveal fi ve fractions of neutral lipids in the sperm of investigated fi sh, which were distributed on the plates as follows: the starting line demonstrated phospholipids, cholesterol, free fatty acids, triacylglycerol and ethers of cholesterol. The adaptive property of sperm was estimated by the level of spermatozoa survival prior to and after cryopreservation. Results. The obtained results of sterlet sperm activity prior to and after cryopreservation demonstrate high quality of native sperm and its considerable deterioration after freezing/thawing. Conclusions. The impact of low temperatures on the lipid composition of reproductive cells of male sterlets (Acipenser ruthenus L) in cryopreservation environment leads to impairments of the phospholipid bilayer of their membranes. It was established that during the thawing of frozen sperm cells which preserved their viability after cryopreservation their motility and capability of fertilizing an ovum were somewhat restored with slowing down of sperm cell motility.

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Location of fishing points of sterlet (Acipenser ruthenus L. 1758) for forensic-ichthyological examination

VESTNIK OF ASTRAKHAN STATE TECHNICAL UNIVERSITY SERIES FISHING INDUSTRY ◽

10.24143/2073-5529-2019-4-70-78 ◽

2019 ◽

Vol 2019 (4) ◽

pp. 70-78

Author(s):

Gleb Igorevich Volosnikov

Keyword(s):

Statistical Processing ◽

Time Interval ◽

Research Station ◽

Meristic Characters ◽

Representative Sampling ◽

Acipenser Ruthenus ◽

Sturgeon Species ◽

Kolmogorov Smirnov ◽

Academy Of Sciences ◽

Sterlet Acipenser Ruthenus

The paper describes the details of the investigative actions taken in terms of the struggle against poaching, where the researchers of the Tobolsk Complex Research Station of the Ural Branch of the Russian Academy of Sciences carry out the forensic ichthyologic examination of illegally caught water bioresources. The researchers often encounter the need to investigate or confirm the alleged place of catching fish species submitted for examination. There have been considered the possibilities to determine the habitat of the caught sturgeon species, in particular sterlet (Acipenser ruthenus L. 1758) using direct comparative analysis and statistical processing of meristic characters of the alleged populations, because there is evidence of existing broodstocks of this species, linked to the specific water areas. The analysis was carried out using 357 specimens of sterlet caught in 4 different areas of the Lower Irtysh. Absence of significant differences in the means of meristic characters in the species of the studied groups can be explained by insufficiently representative sampling, which is frequent in forensic ichthyologic examinations. Nevertheless, according to a number of characteristics, one can speak of a certain heterogeneity of the samples, which indicates the presence of features in the distribution of individuals in the population. Additional statistical data processing by means of Kolmogorov–Smirnov non-parametric two-sample criterion demonstrates a reliable interpopulation difference in meristic characters including the case of scanty sampling. Expanding the study in terms of increasing the number of samples and pairs for comparison, as well as feasibility of analyzing the meristic characters in a longer time interval is being considered.

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