Characterization of Enterobacter intermedius MH8b and its use for the enhancement of heavy metals uptake by Sinapis alba L.

The influence of heavy metals on the germination of crop seeds (Vicia sativa L., Sinapis alba L., and Avena sativa L.) in the presence of Rhodoccocus-biosurfactants was studied.

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Soil contamination in landfills: a case study of a landfill in Czech Republic

Solid Earth ◽

10.5194/se-7-239-2016 ◽

2016 ◽

Vol 7 (1) ◽

pp. 239-247 ◽

Cited By ~ 25

Author(s):

D. Adamcová ◽

M. D. Vaverková ◽

S. Bartoň ◽

Z. Havlíček ◽

E. Břoušková

Keyword(s):

Heavy Metals ◽

Plant Biomass ◽

Sinapis Alba ◽

Soil Samples ◽

Germination Capacity ◽

Threshold Values ◽

Sinapis Alba L ◽

Phytotoxicity Test ◽

Ecotoxicity Tests

Abstract. A phytotoxicity test was determined to assess ecotoxicity of landfill soil. Sinapis alba L. was used as a bioindicator of heavy metals. Soil samples 1–8, which were taken from the landfill body, edge of the landfill body, and its vicinity meet the limits for heavy metals Co, Cd, Pb, and Zn specified in the applicable legislation. Hg and Mn threshold values are not established in legislation, but values have been determined for the needs of the landfill operator. For heavy metals Cr, Cu, and Ni sample 2 exceeded the threshold values, which attained the highest values of all the samples tested for Cr, Cu, and Ni. For Cr and Ni the values were several times higher than values of the other samples. The second highest values for Cr, Cu, and Ni showed sample 6 and 7. Both samples exceeded the set limits. An increase in plant biomass was observed in plants growing on plates with soil samples, but no changes in appearance, slow growth, or necrotic lesions appeared. Ecotoxicity tests show that tested soils (concentration of 50 %) collected from the landfill body, edge of the landfill body, and its vicinity reach high percentage values of germination capacity of seeds of Sinapis alba L. (101–137 %). At a concentration of 25 %, tested soil samples exhibit lower values of germination capacity – in particular samples 3 to 8 – yet the seed germination capacity in all eight samples of tested soils ranges between 86 and 137 %.

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Characterization of Glutamine Synthetase of Roots, Etiolated Cotyledons and Green Leaves from Sinapis alba (L.)

Zeitschrift für Naturforschung C ◽

10.1515/znc-1986-7-809 ◽

1986 ◽

Vol 41 (7-8) ◽

pp. 712-716 ◽

Cited By ~ 9

Author(s):

Remigius Manderscheid ◽

Aloysius Wild

Keyword(s):

Glutamine Synthetase ◽

Crude Extract ◽

Substrate Binding ◽

Sinapis Alba ◽

Kinetic Properties ◽

Temperature Optimum ◽

Ph Optimum ◽

Green Leaves ◽

Sinapis Alba L

Abstract Glutamine synthetase of roots, etiolated cotyledons and green leaves from mustard plants cannot all clearly be separated by DEAE-Sephacel chromatography. However, the enzyme of the roots, etiolated cotyledons and green leaves, respectively, differed in the kinetic properties determined in the crude extract. The root enzyme showed a pH-optimum of about 6.9, a Km value of 3 mᴍ for glutamate and a temperature optimum at 48 °C. Glutamine synthetase of etiolated cotyledons possessed a Km for glutamate of 6 or 12 mᴍ, depending on the dithioerythritol concentration in the homogenisation buffer and a temperature optimum at 46 °C. The enzyme of green leaves was characterized by a temperature optimum at 40 °C, a pH-optimum at about 7.4 and a low glutamate affinity with positive cooperative substrate binding. Based on isolation of chloroplasts and identification of glutamine synthetase the enzyme of green leaves seems to be the chloroplastic form. This enzyme was purified by DEAE-Sephacel, hydroxylapatite and Sephacryl S-300 chromatography. Affinity for glutamate and MgSO4 of the purified enzyme differed from that found in the crude extract. The function of the different isoenzymes is discussed.

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