In vivo genotoxicity testing of the amnesic shellfish poison (domoic acid) in piscine erythrocytes using the micronucleus test and the comet assay

A wide array of antioxidative and anti-inflammatory substances derived from edible plants have been reported to possess chemopreventive and chemoprotective activities. Among the most extensively investigated and well-defined dietary chemopreventives is curcumin. Using the Ames test and in vivo micronucleus test, chemiluminescence test, blastic transformation test, and comet assay, we examined the antimutagenic effects of the chemically identified chemoprotective substance curcumin (diferuloylmethane) in the pure form on mutagenicity induced by three reference mutagens: aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), 2-amino-3-metylimidazo[4,5-f]quinoline (IQ), and N-nitroso-N-metylurea (MNU), and the effect of curcumin on the immunosuppression caused by these mutagens. Curcumin in the pure form showed a clear antimutagenic and immunomodulatory activities on mutagenicity and immunosuppression induced by reference mutagens.  

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First in vivo evaluation of the mutagenic effect of Brazilian green propolis by comet assay and micronucleus test

Food and Chemical Toxicology ◽

10.1016/j.fct.2008.04.001 ◽

2008 ◽

Vol 46 (7) ◽

pp. 2580-2584 ◽

Cited By ~ 23

Author(s):

Alzira Danielly Pereira ◽

Sergio Faloni de Andrade ◽

Mário Sergio de Oliveira Swerts ◽

Edson Luis Maistro

Keyword(s):

Comet Assay ◽

Micronucleus Test ◽

Mutagenic Effect ◽

In Vivo Evaluation ◽

Brazilian Green Propolis

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In vivo genotoxicity of treated water containing the cylindrospermopsin-producer Cylindrospermopsis raciborskii

Journal of Water and Health ◽

10.2166/wh.2014.087 ◽

2014 ◽

Vol 12 (3) ◽

pp. 474-483 ◽

Cited By ~ 3

Author(s):

A. L. Fonseca ◽

J. Da Silva ◽

E. A. Nunes ◽

S. M. F. O. Azevedo ◽

R. M. Soares

Keyword(s):

Bone Marrow ◽

Comet Assay ◽

Bone Marrow Cells ◽

Micronucleus Test ◽

Liver Cells ◽

Cylindrospermopsis Raciborskii ◽

Producer Strain ◽

Treated Water ◽

Marrow Cells

Cylindrospermopsin (CYN) is an alkaloid commonly produced by some cyanobacteria that has been implicated in outbreaks of human illness. The aim of this study was to investigate the genotoxicity of Cylindrospermopsis raciborskii cellular content (including CYN) and its byproducts resulting from chlorination during water treatment. DNA damage in blood and liver cells was analysed by the comet assay and micronucleus test (MN). Mice were injected intraperitoneally with the following treatments: (a) physiological saline, (b) treated water, (c) treated water plus C. raciborskii extract (CYN producer strain, CYPO-011 K), (d) C. raciborskii extract (CYN producer strain, CYPO-011 K), (e) C. raciborskii extract (CYN non producer strain), and (f) treated water plus C. raciborskii extract (CYN non producer strain) extract. After 48 h, samples were taken to perform tests (blood and liver cells to the comet assay and bone marrow to MN test). The CYPO-011 K had a genotoxic and mutagenic effects on liver and bone marrow cells. The group that received chlorine-treated water plus CYPO-011 K also exhibited genotoxic effects in the liver, as well as in the blood, and a mutagenic effect in blood marrow cells. The results emphasise the need of improving CYN monitoring in waters bodies in order to reduce the risk of human exposure.

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