DNA damage and repair after exposure to sevoflurane in vivo, evaluated in Swiss albino mice by the alkaline comet assay and micronucleus test

2010 ◽  
Vol 51 (1) ◽  
pp. 79-86 ◽  
Author(s):  
G. Brozovic ◽  
N. Orsolic ◽  
R. Rozgaj ◽  
V. Kasuba ◽  
F. Knezevic ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Micronucleus Test ◽  
Alkaline Comet Assay ◽  
Dna Damage And Repair ◽  
Swiss Albino Mice ◽  
Albino Mice

The in vivo genotoxicity of cisplatin, isoflurane and halothane evaluated by alkaline comet assay in Swiss albino mice

2011 ◽  
Vol 52 (3) ◽  
pp. 355-361 ◽  
Author(s):  
Gordana Brozovic ◽  
Nada Orsolic ◽  
Fabijan Knezevic ◽  
Anica Horvat Knezevic ◽  
Vesna Benkovic ◽  
...  
Keyword(s):  
Comet Assay ◽  
Alkaline Comet Assay ◽  
Swiss Albino Mice ◽  
Albino Mice

scholarly journals DNA Damaging Effects, Oxidative Stress Responses and Cholinesterase Activity in Blood and Brain of Wistar Rats Exposed to Δ9-Tetrahydrocannabinol

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1560 ◽  
Author(s):  
Nevenka Kopjar ◽  
Nino Fuchs ◽  
Suzana Žunec ◽  
Anja Mikolić ◽  
Vedran Micek ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Comet Assay ◽  
Stress Responses ◽  
Blood Cells ◽  
Genome Stability ◽  
White Blood Cells ◽  
Brain Cells ◽  
Alkaline Comet Assay

Currently we are faced with an ever-growing use of Δ9-tetrahydrocannabinol (THC) preparations, often used as supportive therapies for various malignancies and neurological disorders. As some of illegally distributed forms of such preparations, like cannabis oils and butane hash oil, might contain over 80% of THC, their consumers can become intoxicated or experience various detrimental effects. This fact motivated us for the assessments of THC toxicity in vivo on a Wistar rat model, at a daily oral dose of 7 mg/kg which is comparable to those found in illicit preparations. The main objective of the present study was to establish the magnitude and dynamics of DNA breakage associated with THC exposure in white blood and brain cells of treated rats using the alkaline comet assay. The extent of oxidative stress after acute 24 h exposure to THC was also determined as well as changes in activities of plasma and brain cholinesterases (ChE) in THC-treated and control rats. The DNA of brain cells was more prone to breakage after THC treatment compared to DNA in white blood cells. Even though DNA damage quantified by the alkaline comet assay is subject to repair, its elevated level detected in the brain cells of THC-treated rats was reason for concern. Since neurons do not proliferate, increased levels of DNA damage present threats to these cells in terms of both viability and genome stability, while inefficient DNA repair might lead to their progressive loss. The present study contributes to existing knowledge with evidence that acute exposure to a high THC dose led to low-level DNA damage in white blood cells and brain cells of rats and induced oxidative stress in brain, but did not disturb ChE activities.

scholarly journals Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay

2010 ◽  
Vol 1 (1) ◽  
pp. 8 ◽  
Author(s):  
Halina Lisowska ◽  
Marta Deperas-Kaminska ◽  
Siamak Haghdoost ◽  
Ingela Parmryd ◽  
Andrzej Wojcik
Keyword(s):  
Dna Damage ◽  
T Lymphocytes ◽  
Comet Assay ◽  
Alkaline Comet Assay ◽  
Dna Damage And Repair ◽  
Radiation Induced

scholarly journals In vivo γ-rays Induced Initial DNA Damage and the Effect of Famotidine in Mouse Leukocytes as Assayed by the Alkaline Comet Assay

2007 ◽  
Vol 48 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Hossein MOZDARANI ◽  
Borzo NASIRIAN ◽  
S. Abolghasem HAERI
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Alkaline Comet Assay ◽  
Γ Rays

scholarly journals Hepatocytes, rather than leukocytes reverse DNA damage in vivo induced by whole body y-irradiation of mice, as shown by the alkaline comet assay

2008 ◽  
Vol 41 (2) ◽  
Author(s):  
JUANA PINCHEIRA ◽  
PILAR CARRERA ◽  
KATHERINE MARCELAIN ◽  
CONSUELO DE LA TORRE
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Whole Body ◽  
Alkaline Comet Assay
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