Sulfation pattern of the fucose branch is important for the anticoagulant and antithrombotic activities of fucosylated chondroitin sulfates

2013 ◽  
Vol 1830 (4) ◽  
pp. 3054-3066 ◽  
Author(s):  
Shiguo Chen ◽  
Guoyun Li ◽  
Nian Wu ◽  
Xin Guo ◽  
Ningbo Liao ◽  
...  
Keyword(s):  
Chondroitin Sulfates ◽  
Sulfation Pattern

scholarly journals Microbiological-Chemical Sourced Chondroitin Sulfates Protect Neuroblastoma SH-SY5Y Cells against Oxidative Stress and Are Suitable for Hydrogel-Based Controlled Release

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1816
Author(s):  
Emiliano Bedini ◽  
Alfonso Iadonisi ◽  
Chiara Schiraldi ◽  
Laura Colombo ◽  
Diego Albani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Neuroprotective Effect ◽  
Polymer Networks ◽  
Interpenetrating Polymer Networks ◽  
Ethical Concerns ◽  
Chondroitin Sulfates ◽  
Sulfation Pattern ◽  
Pre Treatment ◽  
Set Up

Chondroitin sulfates (CS) are a class of sulfated glycosaminoglycans involved in many biological processes. Several studies reported their protective effect against neurodegenerative conditions like Alzheimer’s disease. CS are commonly derived from animal sources, but ethical concerns, the risk of contamination with animal proteins, and the difficulty in controlling the sulfation pattern have prompted research towards non-animal sources. Here we exploited two microbiological-chemical sourced CS (i.e., CS-A,C and CS-A,C,K,L) and Carbopol 974P NF/agarose semi-interpenetrating polymer networks (i.e., P.NaOH.0 and P.Ethanol.0) to set up a release system, and tested the neuroprotective role of released CS against H2O2-induced oxidative stress. After assessing that our CS (1–100 µM) require a 3 h pre-treatment for neuroprotection with SH-SY5Y cells, we evaluated whether the autoclave type (i.e., N- or B-type) affects hydrogel viscoelastic properties. We selected B-type autoclaves and repeated the study after loading CS (1 or 0.1 mg CS/0.5 mL gel). After loading 1 mg CS/0.5 mL gel, we evaluated CS release up to 7 days by 1,9-dimethylmethylene blue (DMMB) assay and verified the neuroprotective role of CS-A,C (1 µM) in the supernatants. We observed that CS-A,C exhibits a broader neuroprotective effect than CS-A,C,K,L. Moreover, sulfation pattern affects not only neuroprotection, but also drug release.

scholarly journals Chemoenzymatic preparation of chondroitin sulfates with a defined sulfation pattern

2016 ◽  
Vol 33 ◽  
pp. S43-S44
Author(s):  
Eduardo García-Junceda ◽  
Agatha Bastida ◽  
Ernesto Doncel-Pérez ◽  
Pilar Montero-Calle ◽  
Victor Moreno-Zafra ◽  
...  
Keyword(s):  
Chondroitin Sulfates ◽  
Sulfation Pattern

Glycosaminoglycans from growing antlers of wapiti (Cervus elaphus)

1997 ◽  
Vol 77 (4) ◽  
pp. 715-721 ◽  
Author(s):  
H. H. Sunwoo ◽  
L. Y. M. Sim ◽  
T. Nakano ◽  
R. J. Hudson ◽  
J. S. Sim
Keyword(s):  
Hyaluronic Acid ◽  
Chondroitin Sulfate ◽  
Cervus Elaphus ◽  
Molecular Size ◽  
Enzymatic Digestion ◽  
Gel Chromatography ◽  
Cellulose Acetate Electrophoresis ◽  
Cartilaginous Tissues ◽  
Chondroitin Sulfates ◽  
Velvet Antler

The emerging wapiti industry in North America is based largely on markets for velvet antlers which are used in oriental medicine. Despite the economic opportunity, enthusiasm has been dampened by incomplete understanding of the chemical and pharmacological properties of velvet antler. This study characterizes polysaccharide constituents of glycosaminoglycans in growing antler of wapiti (Cervus elaphus). Glycosaminoglycans were isolated from four sections (tip, upper, middle and base) of growing antlers, and were studied using cellulose acetate electrophoresis, gel electrophoresis, enzymatic digestion and gel chromatography. The tip and upper sections of the antler which are rich in cartilaginous tissues contained chondroitin sulfate as a major glycosaminoglycan with small amounts of hyaluronic acid. In the middle and base sections containing bone and bone marrow, chondroitin sulfate was also a major glycosaminoglycan with small amounts of hyaluronic acid and chondroitinase-ACI resistant materials. More than half of chondroitin sulfate from the middle and base sections had larger molecular size than did the chondroitin sulfates from the tip and upper sections. Key words: Glycosaminoglycans, chondroitin sulfate, antler, wapiti

scholarly journals Isolation and structural studies of heparan sulfates and chondroitin sulfates from three species of molluscs.

1984 ◽  
Vol 259 (3) ◽  
pp. 1431-1435
Author(s):  
H B Nader ◽  
T M Ferreira ◽  
J F Paiva ◽  
M G Medeiros ◽  
S M Jerônimo ◽  
...  
Keyword(s):  
Structural Studies ◽  
Chondroitin Sulfates ◽  
Heparan Sulfates

scholarly journals Analyses of Rabbit Platelet Proteo-Chondroitin Sulfates Localized on the Surface and in the Granules.

1991 ◽  
Vol 14 (11) ◽  
pp. 631-635 ◽  
Author(s):  
Toshio IMANARI ◽  
Toshihiro OGUMA ◽  
Hiroshi AKIYAMA ◽  
Hidenao TOYODA ◽  
Ichiro KOSHIISHI
Keyword(s):  
Rabbit Platelet ◽  
Chondroitin Sulfates

Glycosaminoglycan biosynthesis in the isolated perfused rat lung

1984 ◽  
Vol 57 (6) ◽  
pp. 1648-1654 ◽  
Author(s):  
P. M. Sampson ◽  
R. B. Boyd ◽  
G. G. Pietra ◽  
A. P. Fishman
Keyword(s):  
Hyaluronic Acid ◽  
Heparan Sulfate ◽  
Dermatan Sulfate ◽  
Rat Lung ◽  
Electron Microscopic ◽  
Chondroitin Sulfates ◽  
Isolated Lung ◽  
Microscopic Studies

The suitability of an isolated lung, perfused under carefully monitored conditions, for the study of the biosynthesis of glycosaminoglycans was examined for the rat lung using either [35S]-sulfate or [6-3H]glucosamine. Metabolic and electron-microscopic studies after 3 h of perfusion showed that under the conditions of this study the isolated lung showed no anatomical or metabolic derangements. All glycosaminoglycans normally synthesized in the intact lung were identified. The predominant glycosaminoglycan was heparan sulfate (40% of total). Approximately 14% of the glucosamine incorporated into the glycosaminoglycans was found in hyaluronic acid. Less than 5% of either label was in heparin. The remainder of the synthesized glycosaminoglycans, with the exception of 10% which could not be identified, consisted of the chondroitin sulfates and dermatan sulfate. The relative proportions of the newly synthesized glycosaminoglycans, including the low amounts of heparin, are similar to those found in isolation of endogenous lung glycosaminoglycans. The isolated perfused rat lung appears to be a useful model for the study of glycosaminoglycan biosynthesis by the intact lung.

Detection of chondroitin sulfates and decorin in developing fetal and neonatal rat lung

2002 ◽  
Vol 282 (3) ◽  
pp. L484-L490 ◽  
Author(s):  
Yiqiong Wang ◽  
Kaori Sakamoto ◽  
Jody Khosla ◽  
Philip L. Sannes
Keyword(s):  
Extracellular Matrix ◽  
Chondroitin Sulfate ◽  
Wide Distribution ◽  
Neonatal Rat ◽  
Chondroitin Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Rat Lung ◽  
Adult Rats ◽  
Chondroitin Sulfates ◽  
Monospecific Antibodies

Chondroitin sulfates and their related proteoglycans are components of extracellular matrix that act as key determinants of growth and differentiation characteristics of developing lungs. Changes in their immunohistochemical distribution during progressive organ maturation were examined with monospecific antibodies to chondroitin sulfate, a nonbasement membrane chondroitin sulfate proteoglycan, and the specific chondroitin sulfate-containing proteoglycan decorin in whole fetuses and lungs from newborn and adult rats. Alveolar and airway extracellular matrix immunostained heavily in the prenatal rat for both chondroitin sulfate and chondroitin sulfate proteoglycan, whereas decorin was confined to developing airways and vessels. These sites retained their respective levels of reactivity with all antibodies through 1–10 days postnatal but thereafter became progressively more diminished and focal in alveolar regions. The heavy staining seen early in development was interpreted to reflect a significant and wide distribution of chondroitin sulfates, chondroitin sulfate proteoglycans, and decorin in rapidly growing tissues, whereas the reduced and more focal reactivity observed at later time points coincided with known focal patterns of localization of fibrillar elements of the extracellular matrix and a more differentiated state.

Dietary manganese affects the concentration, composition and sulfation pattern of heparan sulfate glycosaminoglycans in Sprague-Dawley rat aorta

BioMetals ◽  
2006 ◽  
Vol 19 (5) ◽  
pp. 535-546 ◽  
Author(s):  
Anastasia Z. Kalea ◽  
Fotini N. Lamari ◽  
Achilleas D. Theocharis ◽  
Dale A. Schuschke ◽  
Nikos K. Karamanos ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Rat Aorta ◽  
Sprague Dawley ◽  
Sprague Dawley Rat ◽  
Sulfation Pattern
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