A systematic study of yeast sterol biosynthetic protein–protein interactions using the split-ubiquitin system

2005 ◽  
Vol 1737 (2-3) ◽  
pp. 152-160 ◽  
Author(s):  
Caiqing Mo ◽  
Martin Bard
Keyword(s):  
Protein Interactions ◽  
Systematic Study ◽  
Protein Protein Interactions ◽  
Ubiquitin System ◽  
Split Ubiquitin

scholarly journals Mapping Protein-Protein Interactions for the Yeast ABC Transporter Ycf1p by Integrated Split-Ubiquitin Membrane Yeast Two-Hybrid Analysis

2007 ◽  
Vol 26 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Christian M. Paumi ◽  
Javier Menendez ◽  
Anthony Arnoldo ◽  
Kim Engels ◽  
Kavitha Ravee Iyer ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Hybrid Analysis ◽  
Split Ubiquitin ◽  
Two Hybrid

scholarly journals Improved split-ubiquitin screening technique to identify surface membrane protein-protein interactions

BioTechniques ◽  
2015 ◽  
Vol 59 (2) ◽  
Author(s):  
Daniel Ivanusic ◽  
Jürgen J. Heinisch ◽  
Magdalena Eschricht ◽  
Ulrike Laube ◽  
Joachim Denner
Keyword(s):  
Membrane Protein ◽  
Protein Interactions ◽  
Surface Membrane ◽  
Protein Protein Interactions ◽  
Screening Technique ◽  
Split Ubiquitin

scholarly journals The COP9 signalosome: at the interface between signal transduction and ubiquitin-dependent proteolysis

2002 ◽  
Vol 115 (3) ◽  
pp. 467-473 ◽  
Author(s):  
Dawadschargal Bech-Otschir ◽  
Michael Seeger ◽  
Wolfgang Dubiel
Keyword(s):  
Cell Cycle ◽  
Signal Transduction ◽  
Transcription Factors ◽  
Protein Interactions ◽  
Mammalian Cells ◽  
Specific Protein ◽  
26S Proteasome ◽  
Cop9 Signalosome ◽  
Protein Protein Interactions ◽  
Ubiquitin System

Recently the COP9 signalosome (CSN) has become a focus of interest for many researchers, because of its function at the interface between signal transduction and ubiquitin-dependent proteolysis. It is required for the proper progression of the cell cycle in Schizosaccharomyces pombe and is essential for development in plants and Drosophila. However, its function in mammalian cells remains obscure. Although the CSN shares structural similarities with the 26S proteasome lid complex (LID), its functions seem to be different from that of the LID. A variety of CSN-specific protein-protein interactions have been described in mammalian cells. However,it is currently unclear how many reflect true functions of the complex. Two activities associated with the CSN have been identified so far: a protein kinase and a deneddylase. The CSN-associated kinase phosphorylates transcription factors, which determines their stability towards the ubiquitin system. The associated deneddylase regulates the activity of specific SCF E3 ubiquitin ligases. The CSN thus appears to be a platform connecting signalling with proteolysis.

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