scholarly journals Effects of lipid composition on the membrane activity and lipid phase behaviour of Vibrio sp. DSM14379 cells grown at various NaCl concentrations

2005 ◽  
Vol 1712 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Tjaša Danevčič ◽  
Leif Rilfors ◽  
Janez Štrancar ◽  
Göran Lindblom ◽  
David Stopar
2005 ◽  
Vol 44 (5) ◽  
pp. 303-344 ◽  
Author(s):  
Làszló Vigh ◽  
Pablo V. Escribá ◽  
Alois Sonnleitner ◽  
Max Sonnleitner ◽  
Stefano Piotto ◽  
...  

2013 ◽  
Vol 161 ◽  
pp. 151-166 ◽  
Author(s):  
Christoffer Åberg ◽  
Emma Sparr ◽  
Håkan Wennerström

1982 ◽  
Vol 4 (Supplement_1) ◽  
pp. S43-S49 ◽  
Author(s):  
Åke Wieslander ◽  
Anders Christiansson ◽  
Leif Rilfors ◽  
Ali Khan ◽  
B Lennart ◽  
...  

1991 ◽  
Vol 69 (12) ◽  
pp. 863-867 ◽  
Author(s):  
D. A. Mannock ◽  
R. N. McElhaney

We have investigated the physical properties of a homologous series of synthetic, saturated 1,2-di-O-acyl-3-O-(β-D-galactopyranosyl)-sn-glycerols using calorimetry and X-ray diffraction. Unannealed aqueous dispersions of these compounds exhibit a lower temperature, moderately energetic, chain-melting (Lβ/Lα phase transition and a higher temperature, weakly energetic, bilayer/nonbilayer phase transition. On annealing below the Lβ/Lα phase transition, the Lβ phase converts to an LC phase, which may undergo a highly energetic LC/Lα or LC/HII phase transition at very high temperatures on reheating. The temperatures of these phase transitions are higher than those seen in the corresponding α- and β-D-glucosyl diacylglycerols. However, the Lβ/Lα and bilayer/nonbilayer phase transition temperatures of the β-D-galactosyl diacylglycerols are lower than those of the corresponding diacyl phosphatidylethanolamines. These observations are discussed in terms of the hydration and hydrogen bonding properties of their respective headgroups.Key words: differential scanning calorimetry, low-angle x-ray diffraction, glycolipids, galactolipids, lipid phase behaviour.


2010 ◽  
Vol 430 (3) ◽  
pp. 415-423 ◽  
Author(s):  
Janos Juhasz ◽  
James H. Davis ◽  
Frances J. Sharom

Direct visualization of raft-like lo (liquid-ordered) domains in model systems and cells using microscopic techniques requires fluorescence probes with known partitioning preference for one of the phases present. However, fluorescent probes may display dissimilar partitioning preferences in different lipid sys-tems and can also affect the phase behaviour of the host lipid bilayer. Therefore a detailed understanding of the behaviour of fluorescent probes in defined lipid bilayer systems with known phase behaviour is essential before they can be used for identifying domain phase states. Using giant unilamellar vesicles composed of the ternary lipid mixture DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine)/DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine)/cholesterol, for which the phase behaviour is known, we examined nine commonly used fluorescent probes using confocal fluorescence microscopy. The partitioning preference of each probe was assigned either on the basis of quantification of the domain area fractions or by using a well-characterized ld (liquid-disordered)-phase marker. Fluorescent probes were examined both individually and using dual or triple labelling approaches. Most of the probes partitioned individually into the ld phase, whereas only NAP (naphtho[2,3-a]pyrene) and NBD-DPPE [1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl] preferred the lo phase. We found that Rh-DPPE (Lissamine™ rhodamine B–1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine) increased the miscibility transition temperature, Tmix. Interestingly, the partitioning of DiIC18 (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate) was influenced by Bodipy®-PC [2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexa-decanoyl-sn-glycero-3-phosphocholine]. The specific use of each of the fluorescent probes is determined by its photostability, partitioning preference, ability to detect lipid phase separations and induced change in Tmix. We demonstrate the importance of testing a specific fluorescent probe in a given model membrane system, rather than assuming that it labels a particular lipid phase.


The structural organization of biological membranes is largely determined by the weak interactions existing between their components and between these components and their aqueous environment. These interactions are particularly sensitive to changes in temperature and hydration. The factors influencing membrane lipid phase behaviour are briefly reviewed and used to develop a phase-separation model describing the response of biological membranes to stress. The factors affecting the interaction of cryoprotectants with membrane lipids are explored and their role in the stabilization of membrane organization at low temperatures discussed. It is suggested that the basis of their protective action lies in an ability to preserve the balance of interactions between membrane components at low temperatures at a level similar to that existing under physiological conditions.


FEBS Letters ◽  
1983 ◽  
Vol 157 (1) ◽  
pp. 155-158 ◽  
Author(s):  
L.S. Chia ◽  
J.E. Thompson ◽  
M.A. Moscarello
Keyword(s):  

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