Scores for standardization of on-tissue digestion of formalin-fixed paraffin-embedded tissue in MALDI-MS imaging

2017 ◽  
Vol 1865 (7) ◽  
pp. 907-915 ◽  
Author(s):  
Katrin Erich ◽  
Denis A. Sammour ◽  
Alexander Marx ◽  
Carsten Hopf
Keyword(s):  
Maldi Ms ◽  
Ms Imaging ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed ◽  
Tissue Digestion

scholarly journals High-resolution MALDI-FT-ICR MS imaging for the analysis of metabolites from formalin-fixed, paraffin-embedded clinical tissue samples

2015 ◽  
Vol 237 (1) ◽  
pp. 123-132 ◽  
Author(s):  
Achim Buck ◽  
Alice Ly ◽  
Benjamin Balluff ◽  
Na Sun ◽  
Karin Gorzolka ◽  
...  
Keyword(s):  
High Resolution ◽  
Tissue Samples ◽  
Ms Imaging ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Ft Icr Ms ◽  
Formalin Fixed ◽  
Ft Icr

scholarly journals Tissue Digestion for Ferruginous Bodies for Light Microscopy

1998 ◽  
Vol 6 (1) ◽  
pp. 8-11
Author(s):  
Margaret Gondo
Keyword(s):  
Tissue Sample ◽  
Lung Parenchyma ◽  
Asbestos Fibers ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
The Individual ◽  
Ferruginous Bodies ◽  
Formalin Fixed ◽  
Tissue Digestion

Asbestos fibers are commonly called ferruginous bodies due to the iron content of the fibers. After they are inhaled, the asbestos fibers become coated with glycoprotein and hemosiderin, deposited by macrophages.This procedure may be used on either formalin fixed, paraffin embedded blocks or formalin fixed wet tissue. Because this procedure will result in complete destruction of the tissue sample used, it is very important to obtain written approval documenting this fact from the individual requesting this procedure, if the procedure is being done for someone else. The tissue should be from the lung parenchyma. Also, it is a good idea to use sterile, disposable tubes throughout this procedure to limit the possibility of contamination.

scholarly journals Optimal immunoreactivity of keratin proteins in formalin-fixed, paraffin-embedded tissue requires preliminary trypsinization. An immunoperoxidase study of various tumours using polyclonal and monoclonal antibodies.

1985 ◽  
Vol 33 (5) ◽  
pp. 465-473 ◽  
Author(s):  
G S Pinkus ◽  
E M O'Connor ◽  
C L Etheridge ◽  
J M Corson
Keyword(s):  
Monoclonal Antibodies ◽  
Transitional Cell ◽  
Type Ii ◽  
Trypsin Treatment ◽  
Bovine Trypsin ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Keratin Proteins ◽  
Formalin Fixed ◽  
Tissue Digestion

The effect of preliminary trypsinization on the immunoreactivity of keratin proteins in formalin-fixed, paraffin-embedded tissues of a variety of tumors (squamous cell carcinomas, adenocarcinomas, mesotheliomas, and transitional cell carcinomas) was evaluated. Three types of trypsin (Type II and Type IX porcine trypsin and Type III bovine trypsin) and varying concentrations of trypsin were assessed. Immunoreactivity of keratin proteins was determined using rabbit anti-keratin antibodies and monoclonal antibodies (combination of AE1 and AE3) and immunoperoxidase techniques. Preliminary trypsinization was mandatory for optimal immunoreactivity of keratin proteins using either polyclonal or monoclonal antibodies. Excellent results were obtained using Type II porcine trypsin at concentrations of 25 mg/dl for 30-45 min or 50 mg/dl for 20 min, at 37 degrees C. Trypsin treatment with excessive concentrations of enzyme and/or extended incubation times promoted tissue digestion and in some cases, yielded decreased immunoreactivity and altered staining patterns.

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