Estrous cycle modulates the anxiogenic effects of caffeine in the elevated plus maze and light/dark box in female rats

Quercitrin is a well-known flavonoid that is contained in Flos Albiziae, which has been used for the treatment of anxiety. The present study investigated the anxiolytic-like effects of quercitrin in experimental models of anxiety. Compared with the control group, repeated treatment with quercitrin (5.0 and 10.0 mg/kg/day, p.o.) for seven days significantly increased the percentage of entries into and time spent on the open arms of the elevated plus maze. In the light/dark box test, quercitrin exerted an anxiolytic-like effect at 5 and 10 mg/kg. In the marble-burying test, quercitrin (5.0 and 10.0 mg/kg) also exerted an anxiolytic-like effect. Furthermore, quercitrin did not affect spontaneous locomotor activity. The anxiolytic-like effects of quercitrin in the elevated plus maze and light/dark box test were blocked by the serotonin-1A (5-hydroxytryptamine-1A (5-HT1A)) receptor antagonist WAY-100635 (3.0 mg/kg, i.p.) but not by theγ-aminobutyric acid-A (GABAA) receptor antagonist flumazenil (0.5 mg/kg, i.p.). The levels of brain monoamines (5-HT and dopamine) and their metabolites (5-hydroxy-3-indoleacetic acid, 3,4-dihydroxyphenylacetic acid, and homovanillic acid) were decreased after quercitrin treatment. These data suggest that the anxiolytic-like effects of quercitrin might be mediated by 5-HT1Areceptors but not by benzodiazepine site of GABAAreceptors. The results of the neurochemical studies suggest that these effects are mediated by modulation of the levels of monoamine neurotransmitters.

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Dissimilar Anxiety-like Behavior in Prepubertal and Young Adult Female Rats on Acute Exposure to Aluminium

Central Nervous System Agents in Medicinal Chemistry ◽

10.2174/1871524922666211231095507 ◽

2021 ◽

Vol 22 ◽

Author(s):

Trina Sengupta ◽

Sutirtha Ghosh ◽

Archana Gaur T. ◽

Prasunpriya Nayak

Keyword(s):

Body Weight ◽

Young Adult ◽

Adult Female ◽

Developmental Stages ◽

Elevated Plus Maze ◽

Age Groups ◽

Female Rats ◽

Acute Exposure ◽

Adult Rats ◽

Plus Maze

Background: Puberty is a developmental transition in which an estrogenic surge occurs, mediating the release of xenoestrogens, like aluminium. Aluminium’s effect on anxiety in rodents at the different developmental stages is inconsistent. Aims: This study aimed at investigating the effect of the metalloestrogenic property of aluminium on anxiety-like behavioral changes in prepubertal and young adult female rats. Objective: Considering this aim, our objective was to evaluate the anxiety-like behavior by the elevated plus maze in prepubertal and young adult female rats with or without acute exposure to aluminium. Methods: To address this property of aluminium, 5mg/Kg body weight (Al-5) and 10 mg/Kg body weight (Al-10) of aluminium was administered intraperitoneally to female rats at two developmental stages, prepubertal (PP; n = 8 for each dose) and young adult (YA; n = 6 for each dose) for two weeks. Post-treatment, three days behavioral assessment of the rats was done employing elevated plus maze. Results: Reduced escape latency was seen in Al-5, Al-10 pre-pubertal rats, and Al-5 young-adult rats on day 3. A significant reduction in open arm time was seen in the Al-5 young-adult rats. Aluminium treatment in the pre-pubertal rats reduced their head dipping and grooming. Reduced sniffing, head dipping, and stretch-attended posture in the treated young-adult female rats showed that they had impaired risk-taking tendency. Conclusion: Differential effect on the anxiety-like behavior in the pre-pubertal and young-adult female rats might be due to the metalloestrogenic property of aluminium, acting differently on the two age groups.

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Behavioral and pharmacological investigation of anxiety and maternal responsiveness of postpartum female rats in a pup elevated plus maze

Behavioural Brain Research ◽

10.1016/j.bbr.2015.07.010 ◽

2015 ◽

Vol 292 ◽

pp. 414-427 ◽

Cited By ~ 12

Author(s):

Yu Yang ◽

Jingxue Qin ◽

Weihai Chen ◽

Nan Sui ◽

Hong Chen ◽

...

Keyword(s):

Elevated Plus Maze ◽

Female Rats ◽

Pharmacological Investigation ◽

Maternal Responsiveness ◽

Plus Maze

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Prenatal Protein Malnutrition Affects Exploratory Behavior of Female Rats in the Elevated Plus-Maze Test

Physiology & Behavior ◽

10.1016/0031-9384(96)00037-6 ◽

1996 ◽

Vol 60 (2) ◽

pp. 681-684 ◽

Cited By ~ 1

Author(s):

S Almeida

Keyword(s):

Exploratory Behavior ◽

Elevated Plus Maze ◽

Protein Malnutrition ◽

Female Rats ◽

Elevated Plus Maze Test ◽

Maze Test ◽

Plus Maze

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Anxiolytic effect of clonazepam in female rats: Grooming microstructure and elevated plus maze tests

European Journal of Pharmacology ◽

10.1016/j.ejphar.2012.03.038 ◽

2012 ◽

Vol 684 (1-3) ◽

pp. 95-101 ◽

Cited By ~ 22

Author(s):

Maurício S. Nin ◽

Natividade S. Couto-Pereira ◽

Marilise F. Souza ◽

Lucas A. Azeredo ◽

Marcelo K. Ferri ◽

...

Keyword(s):

Elevated Plus Maze ◽

Anxiolytic Effect ◽

Female Rats ◽

Plus Maze

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Modulating Effects of Cholecalciferol Treatment on Estrogen Deficiency-Induced Anxiety-Like Behavior of Adult Female Rats

Folia Medica ◽

10.1515/folmed-2017-0022 ◽

2017 ◽

Vol 59 (2) ◽

pp. 139-158 ◽

Cited By ~ 5

Author(s):

Julia Fedotova ◽

Daria Zarembo ◽

Jozef Dragasek ◽

Martin Caprnda ◽

Peter Kruzliak ◽

...

Keyword(s):

Elevated Plus Maze ◽

Open Field Test ◽

Estrogen Deficiency ◽

Female Rats ◽

Ovx Rats ◽

Plus Maze ◽

17Β Estradiol ◽

Experimental Rat Model ◽

High Doses ◽

Estradiol 17Β

AbstractBackground:Vitamin D can be one of the candidate substances that are used as additional supplementation in the treatment of anxiety-related disorders in women with estrogen imbalance.Materials and methods:The aim of the present study was to examine the effects of chronic cholecalciferol administration (1.0, 2.5 or 5.0 mg/kg/day, s.c.) on the anxiety-like behavior and monoamines levels in the rat hippocampus following ovariectomy in female rats. Cholecalciferol was given to ovariectomized (OVX) rats and OVX rats treated with 17β-estradiol (17β-E2, 0.5 μg/rat, s.c.). The anxiety-like behavior was assessed in the elevated plus maze (EPM) and the light-dark tests (LDT), locomotor and grooming activities were assessed in the open-field test (OFT).Results:Cholecalciferol in high doses alone or in combination with 17β-E2-induced anxiolytic-like effects in OVX and OVX rats treated with 17β-E2as evidenced in the EPM and LDT tests, and increased grooming activity in the OFT test. We found that DA and 5-HT levels increased while 5-HT turnover in the hippocampus decreased in these groups of OVX rats.Conclusion:Our results indicate that cholecalciferol in high doses has a marked anxiolytic-like effect due to an increase in the monoamines levels in the experimental rat model of estrogen deficiency.

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Tamoxifen antagonizes the effects of ovarian hormones to induce anxiety and depression-like behavior in rats

Arquivos de Neuro-Psiquiatria ◽

10.1590/0004-282x20140221 ◽

2015 ◽

Vol 73 (2) ◽

pp. 132-139 ◽

Cited By ~ 13

Author(s):

Hamid Azizi-Malekabadi ◽

Masoume Pourganji ◽

Hoda Zabihi ◽

Mohsen Saeedjalali ◽

Mahmoud Hosseini

Keyword(s):

Sham Group ◽

Forced Swimming Test ◽

Elevated Plus Maze ◽

Ovarian Hormones ◽

Female Rats ◽

Ovariectomized Rats ◽

Anxiety And Depression ◽

Ovarian Hormone ◽

Plus Maze ◽

Swimming Test

The effects of tamoxifen (TAM) on anxiety and depression-like behavior in ovariectomized (OVX) and naïve female rats were investigated. The animals were divided into Sham-TAM, OVX-TAM, Sham and OVX groups. Tamoxifen (1 mg/kg) was administered for 4 weeks. In the forced swimming test, the immobility times in the OVX and Sham-TAM groups were higher than in the Sham group. In the open field, the numbers of central crossings in the OVX and Sham-TAM groups were lower than the number in the Sham group, and the number of peripheral crossings in the OVX group was lower than the number in the Sham group. In the elevated plus maze, the numbers of entries to the open arm among the animals in the Sham-TAM and OVX groups were lower than the number in the Sham group, while the number of entries to the open arm in the OVX-TAM group was higher than the number in the OVX group. It was shown that deletion of ovarian hormones induced anxiety and depression-like behavior. Administration of tamoxifen in naïve rats led to anxiety and depression-like behavior that was comparable with the effects of ovarian hormone deletion. It can be suggested that tamoxifen antagonizes the effects of ovarian hormones. It also seems that tamoxifen has anxiolytic effects on ovariectomized rats.

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