Analysis of the type IV secretion system-dependent cell motility of Helicobacter pylori-infected epithelial cells

2004 ◽  
Vol 322 (3) ◽  
pp. 860-866 ◽  
Author(s):  
Laila Al-Ghoul ◽  
Silja Wessler ◽  
Tanja Hundertmark ◽  
Sabine Krüger ◽  
Wolfgang Fischer ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Cell Motility ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Type Iv ◽  
Dependent Cell

930 HELICOBACTER PYLORI PROMOTES THE DIFFERENTIATION OF PPAR∂-EXPRESSING EPITHELIAL CELLS FROM LRIG+ STEM CELLS IN A CAG TYPE IV SECRETION SYSTEM-DEPENDENT MANNER

2020 ◽  
Vol 158 (6) ◽  
pp. S-185
Author(s):  
Lydia Wroblewski ◽  
Alberto Delgado ◽  
Maria B. Piazuelo ◽  
Judith Romero-Gallo ◽  
Robert J. Coffey ◽  
...  
Keyword(s):  
Stem Cells ◽  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Dependent Manner ◽  
Type Iv

scholarly journals Genes Required for Assembly of Pili Associated with the Helicobacter pylori cag Type IV Secretion System

2014 ◽  
Vol 82 (8) ◽  
pp. 3457-3470 ◽  
Author(s):  
Elizabeth M. Johnson ◽  
Jennifer A. Gaddy ◽  
Bradley J. Voss ◽  
Ewa E. Hennig ◽  
Timothy L. Cover
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Gastric Epithelial Cells ◽  
Content Type ◽  
Type Iv ◽  
Pilus Biogenesis ◽  
H Pylori

ABSTRACTHelicobacter pyloricauses numerous alterations in gastric epithelial cells through processes that are dependent on activity of thecagtype IV secretion system (T4SS). Filamentous structures termed “pili” have been visualized at the interface betweenH. pyloriand gastric epithelial cells, and previous studies suggested that pilus formation is dependent on the presence of thecagpathogenicity island (PAI). Thus far, there has been relatively little effort to identify specific genes that are required for pilus formation, and the role of pili in T4SS function is unclear. In this study, we selected 7 genes in thecagPAI that are known to be required for T4SS function and investigated whether these genes were required for pilus formation.cagT,cagX,cagV,cagM, andcag3mutants were defective in both T4SS function and pilus formation; complemented mutants regained T4SS function and the capacity for pilus formation.cagYandcagCmutants were defective in T4SS function but retained the capacity for pilus formation. These results define a set ofcagPAI genes that are required for both pilus biogenesis and T4SS function and reveal that these processes can be uncoupled in specific mutant strains.

scholarly journals Cholesterol Depletion Reduces Helicobacter pylori CagA Translocation and CagA-Induced Responses in AGS Cells

2008 ◽  
Vol 76 (7) ◽  
pp. 3293-3303 ◽  
Author(s):  
Chih-Ho Lai ◽  
Yun-Chieh Chang ◽  
Shin-Yi Du ◽  
Hung-Jung Wang ◽  
Chun-Hsien Kuo ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Secretion System ◽  
Membrane Dynamics ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Cholesterol Depletion ◽  
Dependent Manner ◽  
Ags Cells ◽  
Type Iv

ABSTRACT Infection with Helicobacter pylori cagA-positive strains is associated with gastritis, ulcerations, and gastric cancer. CagA is translocated into infected epithelial cells by a type IV secretion system and can be tyrosine phosphorylated, inducing signal transduction and motogenic responses in epithelial cells. Cellular cholesterol, a vital component of the membrane, contributes to membrane dynamics and functions and is important in VacA intoxication and phagocyte evasion during H. pylori infection. In this investigation, we showed that cholesterol extraction by methyl-β-cyclodextrin reduced the level of CagA translocation and phosphorylation. Confocal microscope visualization revealed that a significant portion of translocated CagA was colocalized with the raft marker GM1 and c-Src during infection. Moreover, GM1 was rapidly recruited into sites of bacterial attachment by live-cell imaging analysis. CagA and VacA were cofractionated with detergent-resistant membranes (DRMs), suggesting that the distribution of CagA and VacA is associated with rafts in infected cells. Upon cholesterol depletion, the distribution shifted to non-DRMs. Accordingly, the CagA-induced hummingbird phenotype and interleukin-8 induction were blocked by cholesterol depletion. Raft-disrupting agents did not influence bacterial adherence but did significantly reduce internalization activity in AGS cells. Together, these results suggest that delivery of CagA into epithelial cells by the bacterial type IV secretion system is mediated in a cholesterol-dependent manner.

High prevalence of functional type IV secretion system for CagA protein in Japanese clinical Helicobacter pylori

2001 ◽  
Vol 120 (5) ◽  
pp. A652-A653
Author(s):  
Y HIRATA ◽  
S MAEDA ◽  
Y MITUNO ◽  
M AKANUMA ◽  
T KAWABE ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Functional Type ◽  
Caga Protein ◽  
Type Iv ◽  
High Prevalence

scholarly journals 814 – Helicobacter Pylori Induces Aberrant Lrig1 Stem Cell Activity Within the Stomach in a Cag Type Iv Secretion System-Dependent Manner

2019 ◽  
Vol 156 (6) ◽  
pp. S-171-S-172 ◽  
Author(s):  
Lydia Wroblewski ◽  
Eunyoung Choi ◽  
Christine Petersen ◽  
Alberto Delgado ◽  
M. Blanca Piazuelo ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Stem Cell ◽  
Cell Activity ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Dependent Manner ◽  
Type Iv ◽  
Stem Cell Activity

The innate immune glycoprotein lactoferrin represses the Helicobacter pylori cag type IV secretion system

ChemBioChem ◽  
2021 ◽  
Author(s):  
Jacky Lu ◽  
Kathryn Haley ◽  
Jamisha Francis ◽  
Miriam Guevara ◽  
Ryan Doster ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Innate Immune ◽  
Type Iv Secretion System ◽  
Type Iv
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