Mechanical strain-induced c-fos expression in pulmonary epithelial cell line A549

PER cells, a transformed pulmonary epithelial cell line that adhered to a large extent to a fibronectin substratum, were found to be attachment-deficient to collagen I. Although fibronectin can bind to collagen I monomers and polymers, the addition of exogenous fibronectin in the attachment medium induced the adhesion of these cells to collagen I polymers but not to monomers. By adding the transglutaminase of blood coagulation, FXIII, in the presence of fibronectin, the attachment of PER cells to collagen I monomers could be recovered while the minimal concentration of fibronectin needed to promote their adhesion to polymers was lowered. These studies indicate that FXIII enhances the fibronectin-mediated attachment of PER cells to collagen I.

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Modulation of endothelin-1 production by a pulmonary epithelial cell line

Biochemical Pharmacology ◽

10.1016/0006-2952(94)90506-1 ◽

1994 ◽

Vol 48 (11) ◽

pp. 2065-2071 ◽

Cited By ~ 18

Author(s):

Eduardo Calderón ◽

Celso E. Gómez-Sánchez ◽

Eduardo N. Cozza ◽

Mingyi Zhou ◽

Ronald G. Coffey ◽

...

Keyword(s):

Epithelial Cell ◽

Cell Line ◽

Epithelial Cell Line ◽

Endothelin 1 ◽

Pulmonary Epithelial Cell

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Clarithromycin Suppresses Human Respiratory Syncytial Virus Infection-InducedStreptococcus pneumoniaeAdhesion and Cytokine Production in a Pulmonary Epithelial Cell Line

Mediators of Inflammation ◽

10.1155/2012/528568 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Shin-ichi Yokota ◽

Tamaki Okabayashi ◽

Satoshi Hirakawa ◽

Hiroyuki Tsutsumi ◽

Tetsuo Himi ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Epithelial Cell ◽

Cell Line ◽

Human Respiratory Syncytial Virus ◽

Receptor Expression ◽

Host Cells ◽

Epithelial Cell Line ◽

Paf Receptor ◽

Pulmonary Epithelial Cell ◽

Syncytial Virus

Human respiratory syncytial virus (RSV) sometimes causes acute and severe lower respiratory tract illness in infants and young children. RSV strongly upregulates proinflammatory cytokines and the platelet-activating factor (PAF) receptor, which is a receptor forStreptococcus pneumoniae, in the pulmonary epithelial cell line A549. Clarithromycin (CAM), which is an antimicrobial agent and is also known as an immunomodulator, significantly suppressed RSV-induced production of interleukin-6, interleukin-8, and regulated on activation, normal T-cell expressed and secreted (RANTES). CAM also suppressed RSV-induced PAF receptor expression and adhesion of fluorescein-labeledS. pneumoniaecells to A549 cells. The RSV-inducedS. pneumoniaeadhesion was thought to be mediated by the host cell’s PAF receptor. CAM, which exhibits antimicrobial and immunomodulatory activities, was found in this study to suppress the RSV-induced adhesion of respiratory disease-causing bacteria,S. pneumoniae, to host cells. Thus, CAM might suppress immunological disorders and prevent secondary bacterial infections during RSV infection.

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