Signaling pathways of the early differentiation of neural stem cells by neurotrophin-3

Hyperbaric oxygen (HBO) therapy may promote neurological recovery from hypoxic-ischemic encephalopathy (HIE). However, the therapeutic effects of HBO and its associated mechanisms remain unknown. The canonical Wnt/β-catenin signaling pathways and bone morphogenetic protein (BMP) play important roles in mammalian nervous system development. The present study examined whether HBO stimulates the differentiation of neural stem cells (NSCs) and its effect on Wnt3/β-catenin and BMP2 signaling pathways. We showed HBO treatment (2 ATA, 60 min) promoted differentiation of NSCs into neurons and oligodendrocytes in vitro. In addition, rat hypoxic-ischemic brain damage (HIBD) tissue extracts also promoted the differentiation of NSCs into neurons and oligodendrocytes, with the advantage of reducing the number of astrocytes. These effects were most pronounced when these two were combined together. In addition, the expression of Wnt3a, BMP2, and β-catenin nuclear proteins were increased after HBO treatment. However, blockade of Wnt/β-catenin or BMP signaling inhibited NSC differentiation and reduced the expression of Wnt3a, BMP2, and β-catenin nuclear proteins. In conclusion, HBO promotes differentiation of NSCs into neurons and oligodendrocytes and reduced the number of astrocytes in vitro possibly through regulation of Wnt3/β-catenin and BMP2 signaling pathways. HBO may serve as a potential therapeutic strategy for treating HIE.

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Hyperthermia influences fate determination of neural stem cells with lncRNAs alterations in the early differentiation

PLoS ONE ◽

10.1371/journal.pone.0171359 ◽

2017 ◽

Vol 12 (2) ◽

pp. e0171359 ◽

Cited By ~ 3

Author(s):

Lei Wang ◽

Yujia Deng ◽

Da Duan ◽

Shuaiqi Sun ◽

Lite Ge ◽

...

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Fate Determination ◽

Early Differentiation

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Cograft of neural stem cells and schwann cells overexpressing TrkC and neurotrophin-3 respectively after rat spinal cord transection

Biomaterials ◽

10.1016/j.biomaterials.2011.06.036 ◽

2011 ◽

Vol 32 (30) ◽

pp. 7454-7468 ◽

Cited By ~ 41

Author(s):

Jun-Mei Wang ◽

Yuan-Shan Zeng ◽

Jin-Lang Wu ◽

Yan Li ◽

Yang D. Teng

Keyword(s):

Spinal Cord ◽

Stem Cells ◽

Neural Stem Cells ◽

Schwann Cells ◽

Spinal Cord Transection ◽

Rat Spinal Cord ◽

Neurotrophin 3

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Protein signaling pathways in differentiation of neural stem cells

PROTEOMICS ◽

10.1002/pmic.200800096 ◽

2008 ◽

Vol 8 (21) ◽

pp. 4547-4559 ◽

Cited By ~ 15

Author(s):

Helena Skalnikova ◽

Petr Vodicka ◽

Steven Pelech ◽

Jan Motlik ◽

Suresh Jivan Gadher ◽

...

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Signaling Pathways ◽

Protein Signaling

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A Switch From Canonical to Noncanonical Wnt Signaling Mediates Early Differentiation of Human Neural Stem Cells

Stem Cells ◽

10.1002/stem.1807 ◽

2014 ◽

Vol 32 (12) ◽

pp. 3196-3208 ◽

Cited By ~ 36

Author(s):

Nora Bengoa-Vergniory ◽

Irantzu Gorroño-Etxebarria ◽

Itxaso González-Salazar ◽

Robert M. Kypta

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Wnt Signaling ◽

Human Neural Stem Cells ◽

Early Differentiation

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Neurotrophin-3 promotes proliferation and cholinergic neuronal differentiation of bone marrow- derived neural stem cells via notch signaling pathway

Life Sciences ◽

10.1016/j.lfs.2016.10.004 ◽

2016 ◽

Vol 166 ◽

pp. 131-138 ◽

Cited By ~ 19

Author(s):

Yu-hui Yan ◽

Shao-heng Li ◽

Zhong Gao ◽

Sa-feng Zou ◽

Hong-yan Li ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Neural Stem Cells ◽

Notch Signaling ◽

Signaling Pathway ◽

Neuronal Differentiation ◽

Notch Signaling Pathway ◽

Neurotrophin 3

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Resolving the transcriptional transitions associated with oligodendrocyte generation from adult neural stem cells by single cell sequencing

10.1101/2020.12.18.423285 ◽

2020 ◽

Author(s):

Kasum Azim ◽

Filippo Calzolari ◽

Martina Cantone ◽

Rainer Akkermann ◽

Julio Vera ◽

...

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Single Cell ◽

Signaling Pathways ◽

Regulatory Control ◽

Transcriptional Networks ◽

Detailed Knowledge ◽

List Type ◽

Specific Expression ◽

Gene Regulatory

AbstractThe subventricular zone (SVZ) is the largest neurogenic niche in the adult forebrain. Notably, neural stem cells (NSCs) of the SVZ generate not only neurons, but also oligodendrocytes, the myelin-forming cells of the central nervous system. Transcriptomic studies have provided detailed knowledge of the molecular events that regulate neurogenesis, but little is understood about adult oligodendrogenesis from SVZ-NSCs. To address this, we performed in-depth single-cell transcriptomic analyses to resolve the major differences in neuronal and oligodendroglial lineages derived from the adult SVZ. A hallmark of adult oligodendrogenesis was the stage-specific expression of transcriptional modulators that regulate developmental oligodendrogenesis. Notably, divergence of the oligodendroglial lineage was distinguished by Wnt-Notch and angiogenesis-related signaling, whereas G-protein-coupled receptor signaling pathways were the major signature observed in the neurogenic lineage. Moreover, in-depth gene regulatory network analysis identified key stage-specific master regulators of the oligodendrocyte lineage and revealed new mechanisms by which signaling pathways interact with transcriptional networks to control lineage progression. Our work provides an integrated view of the multi-step differentiation process leading from NSCs to mature oligodendrocytes, by linking environmental signals to known and novel transcriptional mechanisms orchestrating oligodendrogenesis.Main pointsDistinct adult NSC populations giving rise to either oligodendrocytes or neurons can be identified by the expression of transcription factors.Gene regulatory control of oligodendrogenesis is a major fate-determinant for their generation.

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