hippocampal slice cultures
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2021 ◽  
Vol 7 (2) ◽  
pp. 019-031
Author(s):  
Andrea Gühna ◽  
Jasmin Traichel ◽  
Lepu Zhou ◽  
Ingrid Haas ◽  
Adrienne W Paton ◽  
...  

A hallmark of neurodegenerative diseases is the accumulation of protein aggregates, the formation of which is prevented by chaperone proteins. BiP is the central chaperone in the endoplasmic reticulum. In this study we investigated the pattern of BiP in tunicamycin-stressed murine organotypic hippocampal slice cultures (OHCs). In stressed OHCs highest apoptotic rates occur in neurons of the CA1 regions and the dentate gyrus, in which we found BiP levels to be lowest. Highest BiP protein levels were found in astrocytes. Cell culture experiments indicated that the stress response of glial cells is faster and stronger than in neuronal cells. We hypothesize that the rapid and pronounced BiP expression in astrocytes helps to maintain the fine-balanced micromilieu necessary for survival of neurons. SubAB is a toxin, which cleaves and inactivates BiP. Low dosages of SubAB did not elicit a specific glial response and apoptosis was not induced in a specific hippocampal subfield. Mild prestressing with SubAB promoted neuronal viability in tunicamycin-treated OHCs. We conclude that preconditioning of hippocampal tissue with stressors that elevate endogenous chaperone levels exert a protective effect thereby promoting neuronal survival. These experiments strengthen the thesis that preconditoning with mild stressors positively affects the survival of neuronal cells.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
James M. McCarthy ◽  
Jasmeet Virdee ◽  
Jessica Brown ◽  
Daniel Ursu ◽  
Zeshan Ahmed ◽  
...  

AbstractIntracellular tau inclusions are a pathological hallmark of Alzheimer's disease, progressive supranuclear palsy, corticobasal degeneration and other sporadic neurodegenerative tauopathies. Recent in vitro and in vivo studies have shown that tau aggregates may spread to neighbouring cells and functionally connected brain regions, where they can seed further tau aggregation. This process is referred to as tau propagation. Here we describe an ex vivo system using organotypic hippocampal slice cultures (OHCs) which recapitulates aspects of this phenomenon. OHCs are explants of hippocampal tissue which may be maintained in culture for months. They maintain their synaptic connections and multicellular 3D architecture whilst also permitting direct control of the environment and direct access for various analysis types. We inoculated OHCs prepared from P301S mouse pups with brain homogenate from terminally ill P301S mice and then examined the slices for viability and the production and localization of insoluble phosphorylated tau. We show that following seeding, phosphorylated insoluble tau accumulate in a time and concentration dependent manner within OHCs. Furthermore, we show the ability of the conformation dependent anti-tau antibody, MC1, to compromise tau accrual in OHCs, thus showcasing the potential of this therapeutic approach and the utility of OHCs as an ex vivo model system for assessing such therapeutics.


Author(s):  
Jasmin Weninger ◽  
Maurice Meseke ◽  
Shaleen Rana ◽  
Eckart Förster

Granule cell dispersion (GCD) has been found in the dentate gyrus (dg) of patients with temporal lobe epilepsy (TLE) and a history of febrile seizures but was also recently observed in pediatric patients that did not suffer from epilepsy. This indicates that GCD might not always be disease related, but instead could reflect normal morphological variation. Thus, distribution of newborn granule cells within the hilar region is part of normal dg development at early stages but could be misinterpreted as pathological GCD. In turn, pathological GCD may be caused, for example, by genetic mutations, such as the reeler mutation. GCD in the reeler mutant goes along with an increased susceptibility to epileptiform activity. Pathological GCD in combination with epilepsy is caused by experimental administration of the glutamate receptor agonist kainic acid in rodents. In consequence, the interpretation of GCD and the role of febrile seizures remain controversial. Here, we asked whether febrile temperatures alone might be sufficient to trigger GCD and used hippocampal slice cultures as in vitro model to analyze the effect of a transient temperature increase on the dg morphology. We found that a heat-shock of 41°C for 6 h was sufficient to induce GCD and degeneration of a fraction of granule cells. Both of these factors, broadening of the granule cell layer (gcl) and increased neuronal cell death within the gcl, contributed to the development of a significantly reduced packaging density of granule cells. In contrast, Reelin expressing Cajal–Retzius (CR) cells in the molecular layer were heat-shock resistant. Thus, their number was not reduced, and we did not detect degenerating CR cells after heat-shock, implying that GCD was not caused by the loss of CR cells. Importantly, the heat-shock-induced deterioration of dg morphology was accompanied by a massive microgliosis, reflecting a robust heat-shock-induced immune response. In contrast, in the study that reported on GCD as a non-specific finding in pediatric patients, no microglia reaction was observed. Thus, our findings underpin the importance of microglia as a marker to distinguish pathological GCD from normal morphological variation.


2021 ◽  
Author(s):  
Emily Petrus ◽  
Galit Saar ◽  
Alexia Daoust ◽  
Steve Dodd ◽  
Alan P. Koretsky

2020 ◽  
Vol 30 (3) ◽  
pp. 589-602 ◽  
Author(s):  
Maren Richter ◽  
Natascha Vidovic ◽  
Knut Biber ◽  
Amalia Dolga ◽  
Carsten Culmsee ◽  
...  

2019 ◽  
Vol 20 (6) ◽  
pp. 1266 ◽  
Author(s):  
Urszula Grabiec ◽  
Tim Hohmann ◽  
Chalid Ghadban ◽  
Candy Rothgänger ◽  
Daniel Wong ◽  
...  

N-arachidonoyl glycine (NAGly) is an endocannabinoid involved in the regulation of different immune cells. It was shown to activate the GPR18 receptor, which was postulated to switch macrophages from cytotoxic to reparative. To study GPR18 expression and neuroprotection after NAGly treatment we used excitotoxically lesioned organotypic hippocampal slice cultures (OHSC). The effect of NAGly was also tested in isolated microglia and astrocytes as these cells play a crucial role during neuronal injury. In the present study, the GPR18 receptor was found in OHSC at mRNA level and was downregulated after N-Methyl-D-aspartate (NMDA) treatment at a single time point. Furthermore, treatment with NAGly reduced neuronal damage and this effect was abolished by GPR18 and cannabinoid receptor (CB)2 receptor antagonists. The activation but not motility of primary microglia and astrocytes was influenced when incubated with NAGly. However, NAGly alone reduced the phosphorylation of Akt but no changes in activation of the p44/42 and p38 MAPK and CREB pathways in BV2 cells could be observed. Given NAGly mediated actions we speculate that GPR18 and its ligand NAGly are modulators of glial and neuronal cells during neuronal damage.


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