The simultaneous downregulation of TRPM7 and MagT1 in human mesenchymal stem cells in vitro: Effects on growth and osteogenic differentiation

2019 ◽  
Vol 513 (1) ◽  
pp. 159-165 ◽  
Author(s):  
Sara Castiglioni ◽  
Valentina Romeo ◽  
Laura Locatelli ◽  
Monica Zocchi ◽  
Silvia Zecchini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
In Vitro Effects

scholarly journals The Phosphate Source Influences Gene Expression and Quality of Mineralization during In Vitro Osteogenic Differentiation of Human Mesenchymal Stem Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e65943 ◽  
Author(s):  
Luisa M. Schäck ◽  
Sandra Noack ◽  
Ramona Winkler ◽  
Gesa Wißmann ◽  
Peter Behrens ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Phosphate Source

Two-layer membranes of calcium phosphate/collagen/PLGA nanofibres: in vitro biomineralisation and osteogenic differentiation of human mesenchymal stem cells

Nanoscale ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 401-409 ◽  
Author(s):  
Nora Hild ◽  
Oliver D. Schneider ◽  
Dirk Mohn ◽  
Norman A. Luechinger ◽  
Fabian M. Koehler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells

scholarly journals TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Sara Castiglioni ◽  
Valentina Romeo ◽  
Laura Locatelli ◽  
Alessandra Cazzaniga ◽  
Jeanette A. M. Maier
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells

scholarly journals In Vitro Uptake of Hydroxyapatite Nanoparticles and Their Effect on Osteogenic Differentiation of Human Mesenchymal Stem Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Xing Yang ◽  
Yuanyuan Li ◽  
Xujie Liu ◽  
Ranran Zhang ◽  
Qingling Feng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Exposure Dose ◽  
Immunofluorescent Staining ◽  
Activity Measurement ◽  
Hydroxyapatite Nanoparticles ◽  
Dose Dependent

There have been many applications in biomedical fields based on hydroxyapatite nanoparticles (HA NPs) over the past decades. However, the biocompatibility of HANPs is affected by exposure dose, particle size, and the way of contact with cells. The objective of this study is to investigate the effect of HA NPs with different sizes on osteogenesis using human mesenchymal stem cells (hMSCs). Three different-sized HA NPs (~50, ~100, and ~150 nm, resp.) were synthesized to study the cytotoxicity, cellular uptake, and effect on osteogenic differentiation of hMSCs. The results clearly showed that each size of HA NPs had dose-dependent cytotoxicity on hMSCs. It was found that HA NPs could be uptaken into hMSCs. The osteogenic differentiation of hMSCs was evaluated through alkaline phosphatase (ALP) activity measurement, ALP staining, immunofluorescent staining for osteopontin (OPN), and real-time polymerase chain reaction (RT-PCR) examination. As expected, HA NPs of all sizes could promote the differentiation of hMSCs towards osteoblast lineage. Among the three sizes, smaller-sized HA NPs (~50 and ~100 nm) appeared to be more effective in stimulating osteogenic differentiation of hMSCs.

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