Production of γ-aminobutyric acid from red kidney bean and barley grain fermentation by Lactobacillus brevis TISTR 860

In the present research, the production potential of gamma aminobutyric acid (GABA) using Lactobacillus brevis PML1 was investigated. In addition, the microorganism viability was examined in MAN, ROGOSA, and SHARPE (MRS) after undergoing high hydrostatic pressure at 100, 200, and 300 MPa for 5, 10, and 15 min. Response surface methodology (RSM) was applied to optimize the production conditions of GABA as well as the bacteria viability. Analysis of variance (ANOVA) indicated that both the independent variables (pressure and time) significantly influenced the dependent ones (GABA and bacteria viability) ( P < 0.05 ). The optimum extraction conditions to maximize the production of GABA included the pressure of 300 MPa and the time of 15 min. The amount of the compound was quantified using thin-layer chromatography (TLC) and spectrophotometry. For the process optimization, a central composite design (CCD) was created using Design Expert with 5 replications at the center point, whereby the highest content of GABA was obtained to be 397.73 ppm which was confirmed by high performance liquid chromatography (HPLC). Moreover, scanning electron microscopy (SEM) was utilized to observe the morphological changes in the microorganism. The results revealed that not only did have Lactobacillus brevis PML1 the potential for the production of GABA under conventional conditions (control sample) but also the content of this bioactive compound could be elevated by optimizing the production parameters.

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PENGARUH SUBSTITUSI TEPUNG BATANG NANAS (ANANAS COMOSUS) DAN RED KIDNEY BEAN (PHASEOLUS VULGARIS) TERHADAP DAYA TERIMA, KADAR PATI RESISTEN & PROTEIN PADA MI INSTAN

Media Gizi Indonesia ◽

10.20473/mgi.v16i2.156-166 ◽

2021 ◽

Vol 16 (2) ◽

pp. 156

Author(s):

Yama Dharma Putera ◽

Annis Catur Adi

Keyword(s):

Metabolic Syndrome ◽

Protein Content ◽

Resistant Starch ◽

Theoretical Calculations ◽

Kidney Bean ◽

Ananas Comosus ◽

Acceptance Test ◽

The Metabolic Syndrome ◽

Red Kidney Bean ◽

Instant Noodle

Instant noodle is one of the most popular food in Indonesia because of the fast preparation and easily accepted by people.. However, the nutritional content and overeating instant noodle, become one of the causes metabolic syndrome which is currently increasing globally. This study was aimed to determine the eff ect of pineapple stem starch fl our and red kidney bean (jogo bean) fl our substitution on wheat fl our towards acceptability, levels of resistant starch and protein in instant noodle. The research was done by using a true experimental with a randomized design consisted of the control formula (F0), F1 substitution formula (5% pineapple stem fl our and 20% jogo bean fl our) and F2 (7% pineapple stem fl our and 28% jogo bean fl our). A total of 27 person were involved as semi-trained panelists, acceptance level was tested by Kruskall-Wallis and continued by the Mann-Whitney if the diff erence appeared signifi cant (α≤ 0.05). Resistant starch and protein content will be compared between theoretical calculations from Indonesia Food database and some research with laboratory tests. Acceptance test results state that the most favored substitution formula by panelists is F2 (28% jogo bean fl our and 7% pineapple stem fl our). The highest protein content and resistant starch was found in F2 (14.4 g and 11.3 g). Signifi cant diff erences were seen between F0 with F1 and F2 in the characteristics of color acceptability (p = 0.001) and texture (p = 0.008). Consumption of instant noodles substitution formula F2 with adding a half egg can meet the recommended consumption of one meal of resistant starch and protein which has benefi ts in preventing the metabolic syndrome.

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