Nanostructured Au-Pt hybrid disk electrodes for enhanced parathyroid hormone detection in human serum

2019 ◽  
Vol 128 ◽  
pp. 165-174 ◽  
Author(s):  
Ajay Kumar Yagati ◽  
Anna Go ◽  
Sachin Ganpat Chavan ◽  
Changyoon Baek ◽  
Min-Ho Lee ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Human Serum ◽  
Disk Electrodes

A radioimmunoassay for human parathyroid hormone utilizing a goat anti-bovine PTH serum

1981 ◽  
Vol 96 (2) ◽  
pp. 215-221 ◽  
Author(s):  
L. E. Mallette
Keyword(s):  
Parathyroid Hormone ◽  
Primary Hyperparathyroidism ◽  
Secondary Hyperparathyroidism ◽  
Human Serum ◽  
Central Region ◽  
Antigenic Site ◽  
Human Parathyroid Hormone ◽  
Domestic Goat ◽  
Standard Serum ◽  
Dilution Curves

Abstract. An antiserum (NG-1) against bovine PTH (bPTH) generated in the domestic goat was characterized for use in the radioimmunoassay of PTH in human serum. When a carboxyterminal fragment of bPTH is used as radioligand, this antiserum detects only an antigenic site in the central region of the hPTH molecule. The synthetic hormone fragment, hPTH-(44-68), will displace 93% of the tracer, after which the addition of intact hPTH causes no further displacement. The assay does not detect the synthetic aminoterminal 1-34 fragment of the bovine or human hormones, nor the carboxyterminal fragment of the human hormone, hPTh-(53-84). Standard curves with bPTH-(1-84) and partially purified hPTH are not parallel, so that hPTH is used as standard. Serum from subjects with uraemia or primary hyperparathyroidism gives dilution curves parallel to that with the hPTH standard. The assay with NG-1 has been applied to the diagnosis of primary and secondary hyperparathyroidism, used to monitor the disappearance of PTH after parathyroidectomy, and for measurement of PTH in selective venous samples.

Assay of Parathyroid Hormone in Human Serum and its Uses

1976 ◽  
Vol 13 (1-6) ◽  
pp. 549-554 ◽  
Author(s):  
J. S. Woodhead ◽  
D. A. Walker
Keyword(s):  
Differential Diagnosis ◽  
Parathyroid Hormone ◽  
Human Serum ◽  
No Value ◽  
Clinical Application ◽  
Calcium Metabolism ◽  
Clinical Problem ◽  
Plasma Calcium ◽  
Biochemical Data ◽  
Health And Disease

The assay of parathyroid hormone has contributed greatly to our understanding of calcium metabolism in health and disease. The most important clinical application of the assay is in the differential diagnosis of hypercalcaemia, which is an increasing clinical problem. PTH assays are of little or no value in the absence of other biochemical data, especially accurate determinations of plasma calcium and phosphate.

Parathyroid Hormone Assay in Primary Hyperparathyroidism: Experiences with a Radioimmunoassay Based on Commercially Available Reagents

1974 ◽  
Vol 20 (3) ◽  
pp. 369-375 ◽  
Author(s):  
M Kleerekoper ◽  
J P Ingham ◽  
S W McCarthy ◽  
S Posen
Keyword(s):  
Parathyroid Hormone ◽  
Primary Hyperparathyroidism ◽  
Human Serum ◽  
Serum Calcium ◽  
Parathyroid Tissue ◽  
Normal Range ◽  
Serum Parathyroid Hormone ◽  
Skeletal Changes ◽  
Parathyroid Hormone Assay ◽  
Positive Correlations

Abstract A radioimmunoassay is described for parathyroid hormone in human serum, in which commercially available reagents are used almost exclusively. This assay can be done by any laboratory with experience in radloimmunoassay. Thirty-two of thirty-three patients with surgically proven primary hyperparathyroidism had detectable concentrations of parathyroid hormone in their serum, and concentrations of the hormone exceeded the normal range in 24 of them. Significant positive correlations were found between pre-operative serum calcium, pre-operative serum parathyroid hormone, and the weight of parathyroid tissue removed at operation. These three parameters were also significantly correlated with severity of the skeletal changes as assessed by semiquantitative histological methods.

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